We extracted genomic DNA from 5-ml whole blood samples by using a commercially available DNA isolation kit (Gentra Genomic DNA Purification Kit; Qiagen, Minneapolis, MIN, USA) in accordance with the manufacturer's protocol. Genotypes were identified with the ultra-high throughput GenomeLab SNPstream system14 (link), which uses multiplex polymerase chain reaction (PCR) in conjunction with tag array single-base extension genotyping technology (Beckman Coulter, Fullerton, CA, USA) and the SNPstream software suite15 (link). PCR amplifications were performed in a PTC-225 Peltier Thermal Cycler (MJ Research, Waltham, MA, USA) with Taq Gold DNA polymerase (Applied Biosystems, Foster City, CA, USA). The sequences of the PCR primers and extension primers are available upon request. Multiplex PCR and genotyping were performed in homogeneous reactions, and the assay results were read by direct two-color fluorescence on the SNPstream Ultra-High Throughput Array Imager. Individual genotypes were generated on the basis of the relative fluorescent intensities for each SNP and processed for graphical review. All genotyping results were reviewed and confirmed manually by experienced researchers.
Gentra genomic dna purification kit
Manufactured by Qiagen
Sourced in United States
The Gentra Genomic DNA Purification Kit is a laboratory equipment product designed for the rapid and efficient extraction and purification of genomic DNA from a variety of sample types. The kit utilizes a proprietary method to isolate high-quality DNA, which can be used for various downstream applications in molecular biology and genetics research.
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Lab products found in correlation
2 protocols using gentra genomic dna purification kit
1
Genomic DNA Isolation and Genotyping
2
Schizophrenia Genetic Mutation Screening
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All subjects were Han Chinese from Taiwan: 516 unrelated patients with schizophrenia (258 males, mean age = 43.0 ± 9.3 years, mean age of onset = 22.0 ± 6.7 years; 258 females, mean age = 48.8 ± 10.2 years, mean age of onset = 22.0 ± 7.6 years) were screened for mutations. Blood samples from patients with schizophrenia was obtained from the Yuli Branch, Taipei Veterans General Hospital. Patients fulfilling the diagnostic criteria for schizophrenia defined by the Diagnostic and Statistical Manual of Mental Disorders-Fourth Edition Tex Revision were recruited for the study. The diagnosis of schizophrenia was based on clinical interviews and the review of medical records by senior psychiatrists with consensus. Organic brain syndrome cases, intellectual disability, substance-related psychosis, and mood disorder with psychotic features were excluded. The study was approved by the institutional review board from the Antai Medical Care Cooperation Antai-Tian-Sheng Memorial Hospital (Approval number: 18-144-A). All patients received a full explanation of the study and provided signed written informed consent. Genomic DNA was isolated from peripheral blood cells using the Gentra Genomic DNA Purification kit (QIAGEN, USA) and stored at −20 °C until use.
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