Anti cd14 af700

Manufactured by BioLegend

Anti-CD14 AF700 is a fluorescently-labeled antibody that binds to the CD14 cell surface receptor. CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein expressed primarily on monocytes and macrophages and functions as a co-receptor for the detection of bacterial lipopolysaccharide (LPS).

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CD14-AF700 (5 citations) Anti-human CD14-AF700 antibody (2 citations)

2 protocols using anti cd14 af700

Comprehensive Immunophenotyping of Immune Cells

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Digested samples were stained with anti-CD3 AF700 (BioLegend 300323, Clone HIT3a), anti-CD14 AF700 (BioLegend 325614, Clone HCD14), anti-CD16 AF700 (BioLegend 302026, clone 3G8), anti-IgD BV510 (BioLegend 348220, Clone IA6–2), anti-IgG BV786 (BD Biosciences 564230, Clone G18–145), anti-IgA PE (Miltenyl Biotech 130–113-476, Clone IS11–8E10), anti-IgM PerCP-Cy5.5 (BioLegend 314512, Clone MHM-88), anti-CD45 FITC(BioLegend 304006, Clone HI30), anti-CD19 BV650 (BioLegend 302238, Clone HIB19), anti-CD27 APC (BioLegend 356410, Clone M-T271), anti-CD38 BV 421 (BioLegend 303526, Clone HIT2), anti-CD20 PE-Cy7 (BioLegend 302312, Clone 2H7), anti-CD69 BV605 (BioLegend 310938, Clone FN50) and Zombie-NIR Fixable Viability Kit (BioLegend 423106). Cell samples were sorted with BD FACSARIA II SORP into 1.5 ml Eppendorf tubes containing 600 μl of RNAzol (MRC RN 190). Flow data was analyzed using FlowJo.

Schartl M., Schlupp I., Schartl A., Meyer M.K., Nanda I., Schmid M., Epplen J.T, & Parzefall J. (1991). On the stability of dispensable constituents of the eukaryotic genome: stability of coding sequences versus truly hypervariable sequences in a clonal vertebrate, the amazon molly, Poecilia formosa.. Proceedings of the National Academy of Sciences of the United States of America, 88(19), 8759-8763.

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Characterizing Dendritic Cell Phenotype

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Before staining, FcγR were blocked using the Human TruStain FcX Solution (BioLegend) for 10 min at room temperature in PBS 2% FBS. Cells were then stained for 15 min at 4°C in PBS 2% FBS with one or more of the following Ab: anti- CD14-AF700 (BioLegend), anti-DC-SIGN-AF647 (BioLegend), anti-CD1a-AF488 (BioLegend), anti-CD83-PE (BioLegend), anti-CD86-Pacific Blue (BioLegend), anti-CD40 PE (BioLegend), anti-CD80 Pacific Blue (BD Biosciences), anti-PD-L1 APC (BioLegend), anti HLA-DR A700 (BioLegend).

Olagnier D., Peri S., Steel C., van Montfoort N., Chiang C., Beljanski V., Slifker M., He Z., Nichols C.N., Lin R., Balachandran S, & Hiscott J. (2014). Cellular Oxidative Stress Response Controls the Antiviral and Apoptotic Programs in Dengue Virus-Infected Dendritic Cells. PLoS Pathogens, 10(12), e1004566.

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