Nicolet is50 spectrometer

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Nicolet iS50 spectrometer is a high-performance Fourier Transform Infrared (FTIR) spectrometer designed for a variety of analytical applications. It is capable of performing infrared spectroscopic analysis to identify and characterize chemical compounds and materials.

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Lab products found in correlation

Escalab 250xi, by Thermo Fisher Scientific (16 mentions) Autosorb 1, by Anton Paar (5 mentions) Xrd 7000, by Shimadzu (5 mentions) Hr800 spectrometer, by Horiba (4 mentions) Deuterium oxide, by Johnson & Johnson (3 mentions) Matlab script, by MathWorks (3 mentions) Omnic software, by Thermo Fisher Scientific (3 mentions) Zetasizer nano zs90, by Malvern Panalytical (2 mentions) D8 advance diffractometer, by Bruker (2 mentions) Sta449f3, by Netzsch (2 mentions) S 4800, by Hitachi (2 mentions) Orbitrap exploris 120, by Thermo Fisher Scientific (2 mentions) Avance 500 mhz, by Bruker (2 mentions) Escalab 250 spectrometer, by Thermo Fisher Scientific (2 mentions) X pert pro, by Malvern Panalytical (2 mentions) Tecnai g2 f30 transmission electron microscope, by Thermo Fisher Scientific (1 mentions) Cary 5000 spectrometer, by Agilent Technologies (1 mentions) Tecnai g2 spirit, by Thermo Fisher Scientific (1 mentions) Matlab, by MathWorks (1 mentions) Optima 8000, by PerkinElmer (1 mentions)

Close spelling variants of this product

Nicolet iS50 FTIR + NIR Spectrometer (2 citations) Thermo Nicolet iS50 FTIR spectrometer (2 citations) Fourier Transform Infra-Red Nicolet™ iS50 Spectrometer (2 citations) Nicolet iS50 model FTIR spectrometer (2 citations) Nicolet iS50 Advanced Spectrometer (2 citations) Nicolet iS50 infrared spectrometer (10 citations) Thermo Nicolet IS50 spectrometer (2 citations) Nicolet iS50 FTIR spectrometer (313 citations) Nicolet iS50 Fourier transform infrared spectrometer (25 citations) Nicolet iS50 FITR Spectrometer (2 citations)

124 protocols using nicolet is50 spectrometer

FTIR Analysis of Photopolymerization Kinetics

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FTIR spectra were taken before and after UV irradiation using a Thermo Fisher Scientific Nicolet^TM iS50 Spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) in attenuated total reflection (ATR) mode. All spectra were acquired in the range of 4000–500 cm⁻¹ by 32 scans and a resolution of 4 cm⁻¹. The measurement of the degree of conversion of the acrylate C=C bond of TMPTA was obtained by considering the area under the C=C band (approx. 1640 cm⁻¹) of the reactive group (

A_{C = C}

) with respect to the area of a reference signal in the spectra, namely the C=O band at 1720 cm⁻¹ (

A_{C = O}

), before irradiation (at time t = 0) and after UV-irradiation (at time t). Percent C=C conversion is calculated as indicated in Equation (1):

C o n v e r s i o n % = (1 - \frac{{|\frac{A_{C = C}}{A_{C = O}}|}_{t}}{{|\frac{A_{C = C}}{A_{C = O}}|}_{t = 0}}) \times 100

The value of conversion for each sample was provided as an average value ± standard deviation of three repetitions.
The insoluble fraction of the samples PEO-XL-E and PEO-XL-C was evaluated through the gel content experiment. Samples were wrapped in a metallic mesh with ultrafine pores and soaked in distilled water, which is a suitable solvent for uncured PEO polymer as well as for TMPTA unreacted monomer. Samples were soaked for 24 h and subsequently were left drying in laboratory conditions for 48 h, and afterwards the mass loss of the samples was calculated.

Fredi G., Kianfar P., Dalle Vacche S., Pegoretti A, & Vitale A. (2021). Electrospun Shape-Stabilized Phase Change Materials Based on Photo-Crosslinked Polyethylene Oxide. Polymers, 13(17), 2979.

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Characterization of Crystalline Powder Samples

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Powder diffractograms were obtained using an X-ray diffractometer (X'Pert Panalytical, Philips, Cambridge, MA) with CuKa radiation at a scanning rate of 1.228 min À1 from 5 to 50 with a measurement step of 0.017 , applying 45 kV at 40 mA to observe the crystallinity of the sample.
2.3.5. Differential scanning calorimetry Differential scanning calorimetry (DSC) measurements were carried out using a DSC-Q200 thermal analyzer (TA Instrument, Guyancourt, France) in a temperature range of 20-210 C (heating rate 5 C.min À1 ) under nitrogen gas with a flow rate of 50 mL min À1 . Samples of raw material and milled powder (2.8-5.5 mg) were placed in a hermetically closed aluminum pan. The heat of fusion (DH F ) and melting temperature (T m ) were calculated using the DSC software (Platinum V R ). The residual crystallinity (v c ) was calculated using Equation ( 1), taking into account the melting enthalpy of 100% crystalline sample (raw material) and ground one (Kumar et al. 2018) .
2.3.6. Fourier-transform infrared (FTIR) spectroscopy FTIR spectra were recorded on a Thermo Scientific Nicolet TM iS50 spectrometer, using Omnic V R software to analyze the data. The samples were placed on the diamond window. The data were recorded in ATR mode in a scan range of 4000-400 cm À1 with a resolution of 4 cm À1 .

Dandignac M., Lacerda S.P., Chamayou A, & Galet L. (2022). Comparison study of physicochemical and biopharmaceutics properties of hydrophobic drugs ground by two dry milling processes. Pharmaceutical development and technology, 27(7).

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FTIR Analysis of Irradiated Specimens

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Specimens for the FTIR study were analyzed after exposure to 70 Gy radiation (IR group) and the corresponding control treatment (C group). FTIR spectra were acquired using a Nicolet iS50 spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) in a spectral range of 4000–400 cm⁻¹ and a spectral resolution of 4 cm⁻¹. The specimens were dried in a vacuum at room temperature for 7 days and fixed on an attenuated total reflectance (ATR) diamond crystal using a special press. The window of the ATR accessory sampled a circular area (d = 2.5 mm) from the center of each specimen. One spectrum per specimen was acquired by averaging 40 consecutive scans.

Turjanski S., Par M., Bergman L., Soče M., Grego T, & Klarić Sever E. (2023). Influence of Ionizing Radiation on Fluoride-Releasing Dental Restorative Materials. Polymers, 15(3), 632.

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Analyzing Protein Structure in Decellularized Samples

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The protein secondary structure of native and decellularized samples was studied using Fourier transform infrared spectroscopy (FTIR). NBPs, DBPs, NPPs, and DPPs flaps (10 × 10 mm², n = 3 for each group) were cut and equilibrated for 3–4 h in deuterium oxide (Janssen, Beerse, Belgium) to reduce the contribution of interfering water bands in the amide-I region [42 (link)]. FTIR investigations were performed using the Nicolet iS-50 spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) in the attenuated total reflectance (ATR) mode. The instrument was equipped with a diamond/ZnSe crystal and pressure arm. The transmittance of both samples and background was each measured using 64 scans and infrared spectra were collected within the 4000–500 cm⁻¹ range, at room temperature. Spectra were then overlapped using a Matlab^® script (Mathworks, Natick, MA, USA) [43 ] to compare the composition of the investigated materials. Amide-I and amide-II bonds, respectively at 1630 cm⁻¹ and 1550 cm⁻¹ [40 (link),43 ], were selected to evaluate the integrity of ECM proteins. Peak transmittance ratio (R) was calculated dividing the intensity of the amide-I peak by the intensity of the amide-II peak.

Todesco M., Imran S.J., Fortunato T.M., Sandrin D., Borile G., Romanato F., Casarin M., Giuggioli G., Conte F., Marchesan M., Gerosa G, & Bagno A. (2022). A New Detergent for the Effective Decellularization of Bovine and Porcine Pericardia. Biomimetics, 7(3), 104.

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FTIR Analysis of Control and Samples

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FTIR spectra of the control and obtained samples were recorded using a Nicolet iS50 spectrometer (Thermo Fisher Scientific Co., Hillsboro, OR, USA). Each measurement was performed using a built-in attenuated total reflectance (ATR) accessory. The analysis was carried out in the wavelength range of 4000–400 cm⁻¹.

Kubiak A., Pajewska-Szmyt M., Kotula M., Leśniewski B., Voronkina A., Rahimi P., Falahi S., Heimler K., Rogoll A., Vogt C., Ereskovsky A., Simon P., Langer E., Springer A., Förste M., Charitos A., Joseph Y., Jesionowski T, & Ehrlich H. (2023). Spongin as a Unique 3D Template for the Development of Functional Iron-Based Composites Using Biomimetic Approach In Vitro. Marine Drugs, 21(9), 460.

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Comprehensive Nanomaterial Characterization by TEM, FTIR, Raman, and XPS

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Transmission electron microscopy (TEM) images were acquired by Tecnai G2 F30 transmission electron microscope (FEI, Hillsboro, OR, United States). BP NSs were observed after being dropped onto a copper grid-coated carbon membrane and air-dried. Fourier transform infrared (FTIR) spectra were recorded with Nicolet iS 50 spectrometer (Thermo Scientific, United States). Raman spectra were recorded at room temperature by LabRAM HR800 high-resolution confocal Raman microscope (HORIBA, United States). X-ray photoelectron spectroscopy was performed with Axis HSi X-ray photoelectron spectroscope (Kratos Ltd., United Kingdom) employing Al Kα radiation (150 W, 1486.6 eV photons) as the excitation source. Zeta potential and size were measured by Malvern Mastersizer 2000 particle size analyzer (Zetasizer Nano ZS90, Malvern Instruments Ltd., United Kingdom). All measurements were conducted three times independently and averaged.

Gao N., Xing C., Wang H., Feng L., Zeng X., Mei L, & Peng Z. (2019). pH-Responsive Dual Drug-Loaded Nanocarriers Based on Poly (2-Ethyl-2-Oxazoline) Modified Black Phosphorus Nanosheets for Cancer Chemo/Photothermal Therapy. Frontiers in Pharmacology, 10, 270.

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Probing Rhombohedral BiFeO3 by FTIR and AFM-IR

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To have a first glimpse of the IR absorption for rhombohedral BiFeO₃, FTIR was carried out based on a Nicolet iS50 spectrometer (Thermo Fisher) under grazing incidence mode with IR beam polarized vertical to the substrate plane. Accordingly, we observed strong absorption at ~500 cm⁻¹, for the rhombohedral continuous thin film. To further distinguish the IR absorption for rhombohedral BiFeO₃ nanocrystals with upward, downward quad-domains and the Solomon topological polar structure, AFM-IR with an ultra-high spatial resolution is carried out as shown in Fig. 4 in the main text.

Wang J., Liang D., Ma J., Fan Y., Ma J., Jafri H.M., Yang H., Zhang Q., Wang Y., Guo C., Dong S., Liu D., Wang X., Hong J., Zhang N., Gu L., Yi D., Zhang J., Lin Y., Chen L.Q., Huang H, & Nan C.W. (2023). Polar Solomon rings in ferroelectric nanocrystals. Nature Communications, 14, 3941.

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FT-IR Spectroscopy of Powdered Samples

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Prior to measurements, powders were blended with dried KBr particles (95 °C, 4 h) and pressed as transparent pellets. Spectra were obtained with a Thermo Fisher Nicolet iS50 spectrometer in transmission mode from 400 to 4000 cm⁻¹ with a spectral resolution of 4 cm⁻¹, with 16 scans per spectrum.

Huang X., Li J., Su X., Fang K., Wang Z., Liu L., Wang H., Yang C, & Wang X. (2021). Remarkable damage in talc caused by electron beam irradiation with a dose of up to 1000 kGy: lattice shrinkage in the Z- and Y-axis and corresponding intrinsic microstructural transformation process speculation. RSC Advances, 11(35), 21870-21884.

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Characterization of Pt-Ni Nanoparticles

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TEM images were conducted
on a FEI Tecnai G² spirit operated at an accelerating voltage
of 120 kV. The real metal loading and composition of the Pt–Ni
NPs was measured by ICP optical emission spectrometry (ARCOS II MV
SPECTRO). XRD patterns were collected on a D8 ADVANCE-diffractometer
(Bruker) equipped with a LynxEye detector and KFL Cu 2K X-ray tube.
UV/vis spectroscopy was carried out on an Agilent Cary 5000 spectrometer.
The FTIR spectra were measured on a Thermo Scientific Nicolet iS50
spectrometer by placing a few drops of the solution on the surface
of a diamond cell.

Qin F., Ma Y., Miao L., Wang Z, & Gan L. (2019). Influence of Metal–Ligand Coordination on the Elemental Growth and Alloying Composition of Pt–Ni Octahedral Nanoparticles for Oxygen Reduction Electrocatalysis. ACS Omega, 4(5), 8305-8311.

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FTIR Characterization of Porcine SIS Tissue

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Native (n = 3) and decellularized (n = 3) porcine SIS tissues were processed for Fourier transform infrared spectroscopy (FTIR) using a Nicolet iS-50 spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) with an Attenuated Total Reflectance (ATR) accessory. The instrument was equipped with diamond/ZnSe crystal and pressure arm. Infrared spectra of the samples and background were collected using 64 scans in the range of 4000-500 cm⁻¹ to characterize the composition of each group of samples.
Data were analysed using a Matlab^® script (Mathworks, Natick, MA, USA) [108 ].

Casarin M., Fortunato T.M., Imran S., Todesco M., Sandrin D., Borile G., Toniolo I., Marchesan M., Gerosa G., Bagno A., Romanato F., Carniel E.L., Morlacco A, & Dal Moro F. (2022). Porcine Small Intestinal Submucosa (SIS) as a Suitable Scaffold for the Creation of a Tissue-Engineered Urinary Conduit: Decellularization, Biomechanical and Biocompatibility Characterization Using New Approaches. International Journal of Molecular Sciences, 23(5), 2826.

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