Au480 automatic biochemical analyzer

Blood samples were taken from the orbit vein and subsequently centrifuged. The serum or plasma obtained was stored at −80°C until biochemical analysis. The serum lipid levels (TC, TG, LDL-C, and HDL-C) were determined by a Beckman Coulter AU480 Automatic Biochemical Analyzer (USA).

An Agilent iFunnel 6550 Q-TOF LC/MS (Agilent Technologies, Santa Clara, CA, USA); AU480 Automatic Biochemical Analyzer (Beckman Coulter Inc., Brea, CA, USA); AT201 Electronic Balance (Mettler Toledo, Mettler, Zurich, Switzerland); and a BX53 microscope (OLYMPUS Corporation, Shinjuku, Tokyo, Japan, Eyepiece type DP72) were used.
MII (10 mL: 50 mg, batch No. 221101104) was purchased from Jiangsu Chia Tai Tianqing Pharmaceutical Co., Ltd. (Lianyungang, China). Diammonium glycyrrhizinate injection (DGI) was purchased from Huarun Shuanghe Limin Pharmaceutical Co., Ltd. (Jinan, China). Acetaminophen injection (20 mL: 2 g, batch No. 20220101) was purchased from Sichuan Jishanzhijia Pharmaceutical Co., Ltd. (Chengdu, China). Methanol, acetonitrile, formic acid and H₂O were all of liquid chromatography–mass spectroscopy (LC–MS) grade (Fairfield, OH, USA), while all the remaining reagents were of analytical grade.

Fasting venous blood was collected from patients with schizophrenia in the early morning after enrollment and from controls at the physical examination visit, centrifuged at 2500 R/min for 20 min, and the upper serum was stored in a −80°C freezer. Serum BDNF levels were measured using sandwich enzyme‐linked immunosorbent assay (ELISA) with dual antibodies (Wuhan Ruixin Bio, Cat. No. rx104552h), and LDL, high‐density lipoprotein (HDL), TG, and TC levels were measured a Beckman au480 automatic biochemical analyzer (USA).

Approximately 2 mL of dog blood through a peripheral vein puncture from the limbs was taken on day 28 (administration period endpoint). To obtain the blood cell counts, the blood sample was transferred into a CBC bottle with EDTA-2 K (3 mL, Vacutainer, BD, USA). Parameters like red blood cell counts (RBC), white blood cell counts (WBC), and hemoglobin (HGB), etc. [23 (link)] were collected through an XT2000i automatic blood cell analyzer (SYSMEX, Hyogo, Japan). To obtain the coagulation function index, the withdrawn blood samples were anticoagulated with sodium citrate (1:9) to separate plasma and analyzed with a Coatron 1800 automatic blood coagulation analyzer (TECO, Hilden, Germany). Parameters like prothrombin time (PT) and activated partial thromboplastin time (APTT) were calculated.
For the measurement of serum biochemistry, at the same time point, another 3 mL of blood samples were withdrawn. Samples were analyzed through an AU480 Automatic Biochemical Analyzer (Beckman, Brea, CA, USA) and a Medica EasyLyte PLUS Electrolyte Analyzer (Medica, Bedford, MA, USA) to calculate parameters like creatinine (CREA), blood urea nitrogen (BUN), blood glucose (GLU), etc.

AU480 automatic biochemical analyzer (Beckman) and LSM 540 SYSTEM laser confocal microscope (Zeiss) were used in this study.