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Nitrocellulose membranes 0.2 mm

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Nitrocellulose membranes (0.2 mm) are a type of porous membrane made of nitrocellulose material. They are designed for use in various laboratory applications, such as protein transfer and immobilization during western blotting procedures.

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Lab products found in correlation

Nupage 4 12 bis tris gradient gel, by Thermo Fisher Scientific (2 mentions) Sheep anti mouse igg, by GE Healthcare (2 mentions) Sds sample buffer, by Boston BioProducts (2 mentions) Anti cd81, by Santa Cruz Biotechnology (2 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) M per mammalian protein extraction reagent, by Thermo Fisher Scientific (2 mentions) Bradford protein assay, by Bio-Rad (2 mentions) Complete mini, by Roche (2 mentions) Precision plus protein standard, by Bio-Rad (2 mentions)

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Nitrocellulose membranes (5040 citations) Nitrocellulose (194 citations) 0.2-mm nitrocellulose membranes (5 citations)

2 protocols using nitrocellulose membranes 0.2 mm

1

Quantitative Western Blot Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples were lysed in M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, catalog No. 78501) buffer containing protease inhibitors (complete, Mini, Roche Diagnostics, catalog No. 04693159001). Protein concentration was determined by Bradford protein assay (Bio-Rad). Thirty micrograms of total protein was boiled for 5 min in SDS sample buffer (Boston BioProducts, catalog No. BP-110R), resolved by the NuPAGE™ gradient 4%–12% Bis-Tris Gel (Invitrogen, catalog No. NP0321BOX) with molecular weight standards (Precision Plus Protein Standards, Bio-Rad, catalog No. 161–0374), and transferred onto nitrocellulose membranes (0.2 mm, Bio-Rad, catalog No. 162–0112). The membranes were blocked with 5% non-fat milk (LabScientific, catalog No. M08425) and incubated overnight with anti-Gluc (rabbit polyclonal, Nanolight, catalog No. 401P) antibody at the dilution 1:1000 and anti-CD81 (mouse monoclonal, Santa Cruz Biotechnology, catalog No. sc-166029) antibody at the dilution 1:200. This was followed by binding of secondary antibodies conjugated to horseradish peroxidase (HRP; donkey, anti-Rabbit IgG, GE Healthcare, NA934–1ML and sheep anti-Mouse IgG, GE Healthcare, NA931–1ML) at the dilution 1:7500 and signal detection with a chemiluminescent substrate (SuperSignal West Pico Chemiluminescent Substrate, Thermo Scientific, catalog No. 34077).
Zaborowski M.P., Lee K., Na Y.J., Sammarco A., Zhang X., Iwanicki M., Cheah P.S., Lin H.Y., Zinter M., Chou C.Y., Fulci G., Tannous B.A., Lai C.P., Birrer M.J., Weissleder R., Lee H, & Breakefield X.O. (2019). Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles. Cell reports, 27(1), 255-268.e6.
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2

Quantitative Western Blot Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples were lysed in M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, catalog No. 78501) buffer containing protease inhibitors (complete, Mini, Roche Diagnostics, catalog No. 04693159001). Protein concentration was determined by Bradford protein assay (Bio-Rad). Thirty micrograms of total protein was boiled for 5 min in SDS sample buffer (Boston BioProducts, catalog No. BP-110R), resolved by the NuPAGE™ gradient 4%–12% Bis-Tris Gel (Invitrogen, catalog No. NP0321BOX) with molecular weight standards (Precision Plus Protein Standards, Bio-Rad, catalog No. 161–0374), and transferred onto nitrocellulose membranes (0.2 mm, Bio-Rad, catalog No. 162–0112). The membranes were blocked with 5% non-fat milk (LabScientific, catalog No. M08425) and incubated overnight with anti-Gluc (rabbit polyclonal, Nanolight, catalog No. 401P) antibody at the dilution 1:1000 and anti-CD81 (mouse monoclonal, Santa Cruz Biotechnology, catalog No. sc-166029) antibody at the dilution 1:200. This was followed by binding of secondary antibodies conjugated to horseradish peroxidase (HRP; donkey, anti-Rabbit IgG, GE Healthcare, NA934–1ML and sheep anti-Mouse IgG, GE Healthcare, NA931–1ML) at the dilution 1:7500 and signal detection with a chemiluminescent substrate (SuperSignal West Pico Chemiluminescent Substrate, Thermo Scientific, catalog No. 34077).
Zaborowski M.P., Lee K., Na Y.J., Sammarco A., Zhang X., Iwanicki M., Cheah P.S., Lin H.Y., Zinter M., Chou C.Y., Fulci G., Tannous B.A., Lai C.P., Birrer M.J., Weissleder R., Lee H, & Breakefield X.O. (2019). Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles. Cell reports, 27(1), 255-268.e6.
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