R asm 580

Manufactured by Lonza

The R-ASM-580 is a laboratory equipment designed for performing analytical tasks. It is a compact and versatile instrument that can be used for a variety of applications in research and development laboratories. The core function of the R-ASM-580 is to provide precise and reliable data collection and analysis capabilities to support scientific investigations and experiments.

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Lab products found in correlation

Antibiotic antimyotic, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions)

4 protocols using r asm 580

Synthetic Peptide Treatment of Human Cells

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Human osteosarcoma cells (U2OS) and fibroblast (BJ-hTERT) cells were cultured in Dulbecco’s modified Eagle medium (DMEM) high glucose with 10% fetal bovine serum (FBS, Hyclone) and 1% penicillin-streptomycin at 37°C in 5% CO₂. The cells were used for experiments after a one-hour treatment at 37°C with a synthetic peptide consisting of PR₂₀ (final concentration 10 µM). Rat vascular smooth muscle cells (SMCs; Lonza, R-ASM-580) were grown in DMEM with 20% FBS and 1x Antibiotic-Antimyotic (Thermo Fisher Scientific).

Shiota T., Nagata R., Kikuchi S., Nanaura H., Matsubayashi M., Nakanishi M., Kobashigawa S., Isozumi N., Kiriyama T., Nagayama K., Sugie K., Yamashiro Y, & Mori E. (2022). C9orf72-Derived Proline:Arginine Poly-Dipeptides Modulate Cytoskeleton and Mechanical Stress Response. Frontiers in Cell and Developmental Biology, 10, 750829.

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Culturing Rat Vascular Smooth Muscle Cells

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rVSMC primary cells (Lonza R-ASM-580; 3001900115) were cultured following the procedures described by others with slight modifications [12 , 14 (link)]. Cells were cultured in a humidified atmosphere at 37°C and 5% CO₂. Standard culture medium consisted of DMEM (Gibco 1 1966- 500), streptomycin/penicillin (Gibco 1 5630-080), and 0.3% DSMO (Sigma-Aldrich D2650), supplemented with 20% fetal bovine serum (FBS, SERADIGM 1 500-500). Cell layers were detached with 0.05% trypsin/0.02% EDTA solution (GIBCO 25200). Cells were maintained at 70–80% confluence by passing as needed. A portion of the rVSMCs was stored for later use under frozen conditions using 90% FBS (Seradigm 1 500-500) and 10% DMSO (Sigma D2650).

Mehansho H., Majeti S, & Tzeghai G. (2021). Prevention of Vascular Calcification by Magnesium and Selected Polyphenols. Advances in Preventive Medicine, 2021, 6686597.

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Culturing Primary Rat Aortic Cells in Plasmax Media

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Primary rat aortic endothelial cells (Cell Biologics, RN-6052) and rat aortic smooth muscle cells (Lonza, R-ASM-580) were cultured in physiologically representative media (Plasmax) formulated as described in [21] (link). Plasmax media was supplemented with 10% fetal bovine serum (Sigma, F1051), 1% penicillin/streptomycin (Sigma, P4333), 6.5 mM L-glutamine and 1 mM sodium pyruvate to ensure adequate growth of primary cells. Cells were incubated in physiologically relevant oxygen levels within a 5% CO₂/ 5% O₂ humidified incubator (Keeley and Mann 2019). Sub-culture was performed with 0.25% tryspin/EDTA solution (Sigma, T4049). Media was refreshed every 24 h. Cells were cultured for 7 days at a final ZnSO₄ concentration of 0 µM (Control), 5 µM, 50 µM.

Bagshaw O.R., Moradi F., Moffatt C.S., Hettwer H.A., Liang P., Goldman J., Drelich J.W, & Stuart J.A. (2021). Bioabsorbable metal zinc differentially affects mitochondria in vascular endothelial and smooth muscle cells. Biomaterials and Biosystems, 4, 100027.

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Nifedipine Modulation of Ang II-Induced Superoxide Production

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Rat aortic smooth muscle cells (Lonza, R-ASM-580) between passage 4 and 10 were cultured in DMEM containing 10% fetal bovine serum (FBS) until confluence, and then starved with media containing 5% FBS overnight. The cells were pretreated with either 25 or 100 nmol/L nifedipine for 30 min prior to exposure to Ang II (100 nmol/L) for 30 min. The cells were harvested and suspended in homogenization buffer (Tris 50 mmol/L, EDTA 0.1 mol/L, EGTA 0.1 mmol/L, protease inhibitor cocktail, pH 7.4), sonicated 5 sec for 10 times on ice. Then the cell lysate was centrifuged at 3,000 rpm for 5 min at 4°C. The supernatant was again centrifuged at 50,000 rpm for 90 min at 4°C. The pellet was collected and resuspended in lysis buffer. Superoxide production was measured from this membrane fraction in the presence or absence of freshly prepared 100 mmol/L NADPH, using ESR. NADPH oxidase activity was calculated as the difference between the two measurements.

Miao X.N., Siu K.L, & Cai H. (2015). Nifedipine Attenuation of Abdominal Aortic Aneurysm in Hypertensive and non-Hypertensive Mice: Mechanisms and Implications. Journal of molecular and cellular cardiology, 87, 152-159.

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