Advia 1800

Manufactured by Siemens

Sourced in Germany, United States, United Kingdom, Japan, Denmark

The ADVIA 1800 is a clinical chemistry analyzer designed for use in medical laboratories. It is capable of performing a wide range of common clinical chemistry tests on patient samples. The ADVIA 1800 automates the analysis process, providing efficient and accurate results to support patient diagnosis and treatment.

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Close spelling variants of this product

ADVIA 1800 Chemistry System (73 citations) ADVIA 1800 Clinical Chemistry System (25 citations) Advia 1800 analyzer (14 citations) ADVIA 1800 Auto Analyzer (14 citations) ADVIA 1800 system (9 citations) Advia 1800 Chemistry Analyzer (8 citations) Advia 1600/1800 (7 citations) ADVIA 1800 Clinical Chemistry analyzer (7 citations) ADVIA 1650 and 1800 (6 citations) Clinical Methods for ADVIA 1800 (3 citations)

284 protocols using advia 1800

Cardiovascular Biomarkers in Healthy Adults

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In this study, 30 men under 45 years of age and 27 women under 55 years of age were examined after approval by the Ethics Committee. The two groups did not differ significantly in BMI. Probands without signs of inflammation, atherosclerosis, or kidney damage (n=21) were selected as a control group.
For all 3 groups, a physical examination was carried out. This included medical history, ECG and echocardiog-raphy. The following parameters were investigated in the blood plasma immediately after collection (Li-He): cTnI (Siemenes, Centaur), NT-proBNP (Siemenes, Cantaur), CRP (Siemens, Advia 1800), creatinine (Siemens, Advia 1800), ALT (Siemens, Advia 1800), AST (Siemens, Advia 1800), bilirubin (Siemens, Advia 1800), Na, K, Cl (Siemens, Advia 1800) ), at the same time, aliquots of blood samples were frozen at -80 °C. Visfatin was measured from these samples (BioVendor, ELISA, Czech Republic).
Statistcal analysis was cared out using the Medcalc program (Belgium). In addition to descriptive statisticsan ROC analysis was performed.

Stejskal D., Sigutova R., Svestak M., Vaclavik J., Kusnierova P, & Svagera Z. (2020). Measurement of novel adipokine visfatin in young patients with acute myocardial infarction. Clinical testing of a new ELISA. Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia, 164(2).

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Comprehensive Blood Chemistry Analysis

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The blood samples were analyzed using spectrophotometry (filters 340–620 nm) with Advia 1800 Clinical Chemistry System (Siemens, Munich, Germany). Glucose-hexocinase_3 (GLUH_3, Advia Chemistry, Siemens, Munich, Germany), HbA1c 3M (Advia 1800, Siemens, Munich, Germany), uric acid (Uricase/Peroxidase, Advia 1800, Siemens, Munich, Germany), creatinine (alkaline picrate, Advia 1800, Siemens, Munich, Germany), triglycerides (GPO-PAP method, Advia 1800, Siemens, Munich, Germany), total-, LDL-, and HDL-cholesterol (catalase method, Advia 1800, Siemens, Munich, Germany).

Robles-Cabrera A., Torres-Arellano J.M., Fossion R, & Lerma C. (2021). Dependence of Heart Rate Variability Indices on the Mean Heart Rate in Women with Well-Controlled Type 2 Diabetes. Journal of Clinical Medicine, 10(19), 4386.

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Plasma Protein Concentration Measurements

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Plasma albumin concentrations were measured by using bromocresol green-binding and spectrophotometrical determination at 596 nm (ADVIA 1800; Siemens Medical Solutions, Tarrytown, NY). Plasma total protein concentrations were measured by using an ADVIA 1800 analyzer (Siemens Medical Solutions, Erlangen, Germany). Plasma Hp concentrations were measured by using a spectrophotometric assay according to the manufacturer's guidelines (TP-801; Tridelta Developments Ltd., Kildare, Ireland). Fibrinogen concentrations in plasma were measured by using a particle-bound polyclonal rabbit anti-fibrinogen antibody (Q 0122; DakoCytomation, Glostrup, Denmark), followed by a turbidimetric assay (ADVIA 1800; Siemens Medical Solutions). All intra-assay CV were ≤3%, and inter-assay CV were ≤4%.
Plasma SAA concentrations were determined by ELISA (TP-802; Tridelta Developments Ltd.) according to the manufacturer's guidelines. Plasma IgG, IgM, and IgA concentrations were determined by ELISA (E11-118, E11-101, and E11-131 respectively; Bethyl Laboratories Inc., Montgomery, TX) according to the manufacturer's guidelines. All intra-assay CV were ≤5%, and inter-assay CV were ≤6%.

Samarasinghe M.B., Sehested J., Larsen T, & Hernández-Castellano L.E. (2020). Oral administration of lipopolysaccharides from Escherichia coli (serotype O111:B4) does not induce an effective systemic immune response in milk-fed Holstein calves. Journal of dairy science, 103(6).

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Standardized Biochemical Analyses in Clinical Research

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Biochemistry was assessed from blood samples collected after overnight fasting during the hospitalisation period. Glycated haemoglobin levels were determined using cation-exchange high-performance liquid chromatography (National Glycohemoglobin Standardization Program certificated; G8, TOSOH, Tokyo, Japan). Lipid levels were determined using enzymatic methods (Advia 1800; Siemens, New York, USA). Creatinine levels were determined using the Jaffé method (Advia 1800; Siemens). Urine protein levels were determined using the dye-binding assay, and urine albumin levels were assessed using the immune-turbidimetric method (Advia 1800; Siemens).

Li Y.H., Sheu W.H, & Lee I.T. (2018). Effects of retinopathy and chronic kidney disease on long-term mortality in type 2 diabetic inpatients with normal urinary albumin or protein: a retrospective cohort study. BMJ Open, 8(7), e021655.

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Quantifying Plasma Immunoglobulins and Acute-Phase Proteins

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Plasma concentrations of IgG, IgM, and IgA were determined by ELISA (E11-118, E11-101, and E11-131 respectively; Bethyl Laboratories Inc., Montgomery, TX) according to the manufacturer's guidelines. All intra-assay coefficients of variation (CV) were ≤5%, and inter-assay CV were ≤ 6%.
Plasma total protein concentrations were determined using an ADVIA 1800 analyzer (Siemens Medical Solutions, Erlangen, Germany). Albumin concentrations in plasma were measured by bromocresol green-binding and spectrophotometric determination at 596 nm (ADVIA 1800; Siemens Medical Solutions). Fibrinogen concentrations in plasma were measured by a particlebound polyclonal rabbit anti-fibrinogen antibody (Q 0122; DakoCytomation, Glostrup, Denmark), followed by a turbidimetric assay (ADVIA 1800; Siemens Medical Solutions). A spectrophotometric assay was used to analyze plasma haptoglobin concentrations, following the manufacturer's guidelines (TP-801; Tridelta Developments Ltd., Kildare, Ireland). For the abovementioned analyses, all intra-assay CV were ≤3% and inter-assay CV were ≤4%. Serum amyloid A (SAA) concentrations in plasma were determined by ELISA (TP-802; Tridelta Developments Ltd.) according to the manufacturer's guidelines. All intra-assay CV for SAA were ≤ 5%, and inter-assay CV were ≤ 6%.

Samarasinghe M.B., Sehested J., Weisbjerg M.R., Vestergaard M, & Hernández-Castellano L.E. (2021). Milk supplemented with dried seaweed affects the systemic innate immune response in preweaning dairy calves. Journal of dairy science, 104(3).

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Serum Lipid Analysis via Colorimetric Methods

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Serum lipid profiles were analyzed using the following KoNEHS data [21 ]. Total-C was analyzed at a wavelength of 505/694 nm using colorimetric analysis (colorimetry, enzymatic method, ADVIA 1800, Siemens). A reagent reaction method is an endpoint method (one reagent) that measures absorbance at the end of the specimen and reagent reaction. HDL-C was measured through analyzing quinonimine produced after a peroxidase reaction with hydrogen peroxide, produced through acting on a cholesterol oxidase at a wavelength of 596 nm, with a colorimetric analysis (colorimetry, elimination/catalase method, ADVIA 1800, Siemens). TG levels were measured by analyzing hydrolyzed glycerol and produced by lipoprotein lipase at a wavelength of 505/694 nm with a colorimetric analysis (colorimetry, GPO Trinder without serum blank method, ADVIA 1800, Siemens). Since LDL-C was not measured directly in the survey, it was obtained using the Friedewald equation. Therefore, values with a TG of ≥ 400 mg/dL could not be used in the LDL-C formula, and those samples were treated as missing values.

Sohn S.H., Heo H.C., Jo S., Park C, & Sakong J. (2020). The association between mercury concentrations and lipid profiles in the Korean National Environmental Health Survey (KoNEHS) cycle 3. Annals of Occupational and Environmental Medicine, 32, e19.

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Comprehensive Metabolic Screening Protocol

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The health status of the participants was verified by the fasting values of clinical markers, such as total cholesterol, VLDL, LDL, HDL, triacylglyceride, glucose, uric acid, urea, creatinine, and high-sensitivity C-reactive protein (hs-CRP). The total cholesterol, triacylglyceride, uric acid, urea, glucose, and creatinine were measured using wet chemistry (except for LDL that was calculated from the Friedewald equation) (Advia 1800, Siemens, Erlangen, Germany). The hs-CRP was quantified by turbidimetry (Advia 1800, Siemens, Erlangen, Germany).

Castro A., Signini É.F., De Oliveira J.M., Di Medeiros Leal M.C., Rehder-Santos P., Millan-Mattos J.C., Minatel V., Pantoni C.B., Oliveira R.V., Catai A.M, & Ferreira A.G. (2022). The Aging Process: A Metabolomics Perspective. Molecules, 27(24), 8656.

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Analytical Procedures for Blood Chemistry

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The participant blood samples were collected in EDTA-containing tubes. After mixing, the blood samples were aliquoted into cryo-tubes and stored at −20 °C. The blood chemistry markers were measured by Seoul Clinical Laboratories (SCL, Yongin, South Korea), with a reference laboratory service [18 ]. Briefly, the serum concentrations of total cholesterol, high-density lipoprotein (HDL) cholesterol, and triglycerides (TG) were measured by an enzymatic method using auto analyzer ADVIA 1800 (Siemens Medical Solutions, USA). Serum low-density lipoprotein (LDL) cholesterol concentrations were calculated from Friedewald’s equation [19 (link)]. Calculated LDL values less than zero were designated as 0 (n = 37). The hepatic enzymes (ALT, AST, and GGT) were measured on an auto-analyzer ADVIA 1800 (Siemens Medical Solutions, Malvern, PA, USA).
Total Hg was measured by flow injection cold-vapor atomic absorption spectrometry (DMA 80, Milestone, Bergamo, Italy) using whole blood samples. The limit of detection (LOD) for blood Hg was 0.10 μg/L. A value below the LOD (n = 1) was included as LOD divided by the square root of 2. External quality control was performed twice per year by the Korean Association of Quality Assurance for Clinical Laboratory (KSLM) and the German External Quality Assessment Scheme for analysis of heavy metals in biological materials (G-EQUAS) [17 (link)].

Lee S., Cho S.R., Jeong I., Park J.B., Shin M.Y., Kim S, & Kim J.H. (2020). Mercury Exposure and Associations with Hyperlipidemia and Elevated Liver Enzymes: A Nationwide Cross-Sectional Survey. Toxics, 8(3), 47.

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Metabolic and Inflammatory Biomarkers

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Blood samples were collected from the antecubital vein
after over 8 hr on fasting. All analyses were performed at a single laboratory center (Seoul Clinical Laboratories R&D Center, Seoul, Korea). Fasting blood glucose and creatinine level was determined by a colorimetry method (ADVIA 1800; Siemens, Tarrytown, NY, USA). HbA1c was determined using high-performance liquid chromatography with a Variant II Turbo (Bio-Rad Laboratories, Hercules, CA, USA). Fasting insulin levels were determined by a radioimmunoassay with SR-300 apparatus (Stratec; Birkenfeld, Rhineland-Palatinate, Germany). HOMA-IR was used to evaluate IR: HOMA-IR=fasting glucose (mg/dL)×fasting insulin (µIU/mL)/405.[12 (link)] Total cholesterol, high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, and high-sensitivity C-reactive protein (hs-CRP) levels were assayed by enzymatic methods (ADVIA 1800; Siemens). Serum 25-hydroxy-vitamin D (25[OH]D) level was assessed by a chemiluminescence immunoassay (DiaSorin, Dietzenbach, Germany). The Chronic Kidney Disease-Epidemiology equation was used to calculate the estimated glomerular filtration rate.[13 (link)]

Yang J., Hong N., Shim J.S., Rhee Y, & Kim H.C. (2018). Association of Insulin Resistance with Lower Bone Volume and Strength Index of the Proximal Femur in Nondiabetic Postmenopausal Women. Journal of Bone Metabolism, 25(2), 123-132.

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Rabbit Blood Biochemical Analysis

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Blood (3.0 ml) was collected from rabbit ears into vacutainer® vials and centrifuged at 3000 rpm for 10 min. Total serum glucose, cholesterol, TG, AST, ALT, and free fatty acid levels were measured by enzymatic colorimetric methods using an automatic analyser (ADVIA 1800; Siemens, Tarrytown, NY, USA). HDL-C and LDL-C were measured by elimination/catalase methods using an automatic analyser (ADVIA 1800; Siemens). Insulin was detected by a chemiluminescence method using an autoanalyser (ADVA Centaur; Siemens).

Chen Y.H., Lin W.W., Liu C.S., Hsu L.S., Lin Y.M, & Su S.L. (2014). Caveolin-1 Provides Palliation for Adverse Hepatic Reactions in Hypercholesterolemic Rabbits. PLoS ONE, 9(1), e71862.

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