Tsq quantum access max

Manufactured by Thermo Fisher Scientific

Sourced in United States, Germany, Switzerland

The TSQ Quantum Access Max is a triple quadrupole mass spectrometer designed for high-performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography (UHPLC) applications. It features advanced ion optics and a high-resolution mass analyzer to provide accurate and sensitive quantitative and qualitative analysis of a wide range of analytes.

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88 protocols using tsq quantum access max

Phenolic Profile Analysis of Grape Samples

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Determination of the phenolic profile in the analyzed grape samples was performed on a Dionex Ultimate 3000 ultra-high-performance liquid chromatograph (UHPLC, Thermo Fisher Scientific, Bremen, Germany) with an ultraviolet multi-diode detector (DAD) and connected to a triple quadrupole weight spectrometer (TSQ Quantum Access MAX, Thermo Fisher Scientific, Basel, Switzerland). Separation was achieved on a Syncronis C18 column (100 × 2.1 mm, 1.7 lm particle size) at 40 °C. All experimental conditions were previously described by [83 (link)].
Detection of polyphenols was acquired in the negative mode on a TSQ Quantum Access MAX triple-quadrupole weight spectrometer (Thermo Fisher Scientific, Basel, Switzerland) with a heated electrospray ionization (HESI) source. The parameters and ion source settings were the same as in [83 (link)]. For instrument control, Xcalibur software 2.2 (Thermo Fisher, Bremen, Germany) was used, while phenolic compounds were identified by direct comparison with commercial standards. The polyphenols content was expressed as mg/kg.

Jovanović-Cvetković T., Sredojević M., Natić M., Grbić R., Akšić M.F., Ercisli S, & Cvetković M. (2023). Exploration and Comparison of the Behavior of Some Indigenous and International Varieties (Vitis vinifera L.) Grown in Climatic Conditions of Herzegovina: The Influence of Variety and Vintage on Physico-Chemical Characteristics of Grapes. Plants, 12(4), 695.

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Quantification of DPS-2 by LC-MS/MS

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The liquid chromatography–mass spectrometry (LC MS-MS) procedure was used to quantify DPS-2 in all samples obtained through ex vivo permeation study and differential tape stripping. In the present study, samples that contained DPS-2 were analyzed on a Thermo Scientific Accela 1000 UPLC system coupled to a Thermo Scientific TSQ Quantum Access MAX triple quadrupole mass spectrometer. The analysis was performed on a UHPLC chromatograph ACELLA (Thermo Fisher Scientific Inc., Madison, WI, USA), coupled to a triple quadrupole mass spectrometer TSQ Quantum Access MAX (Thermo Fisher Scientific Inc., Madison, WI, USA) with a heated electrospray ionization (HESI) interface. The column was a Zorbax Eclipse XDB C18 (150 mm × 4.6 mm, 5 µm particle size). The mobile phase was acetonitrile/0.1% formic acid = 60:40 (v/v), the flow rate was 0.5 mL min⁻¹, the column temperature was set to 30 °C, and the injection volume was 10 µL. DPS-2 was detected and quantified in positive HESI mode (m/z = 504.4).

Theochari I., Ilic T., Nicolic I., Dobricic V., Tenchiou A., Papahatjis D., Savic S., Xenakis A., Papadimitriou V, & Pletsa V. (2021). Biological Evaluation of Oil-in-Water Microemulsions as Carriers of Benzothiophene Analogues for Dermal Applications. Biomimetics, 6(1), 10.

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Quantitative Analysis of Target Analytes

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A triple quadrupole mass spectrometer (Thermo Scientific TSQ Quantum Access MAX) equipped with a heated-electrospray ionization (HESI-II) probe was used. The source-dependent parameters were as follows: spray voltage (3000 V); sheath gas pressure (25 psi); auxiliary gas pressure (10 arbitrary units); skimmer offset (0 V), ion transfer tube temperature (270°C), vaporizer temperature (40°C); high purity argon (>99.98%) which was used as the collision gas (1.5 mTorr). The mass spectrometer operated at a resolution of 0.7 Da in negative selected reaction monitoring (SRM) mode. Table 2 lists the MS/MS transitions, tube lens offset, and collision energies applied for the different target analytes and isotope labelled standards. The Xcalibur 2.1 (Thermo Scientific) was used for instrument control, data acquisition, and processing.

Mazzoni M., Rusconi M., Valsecchi S., Martins C.P, & Polesello S. (2015). An On-Line Solid Phase Extraction-Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Perfluoroalkyl Acids in Drinking and Surface Waters. Journal of Analytical Methods in Chemistry, 2015, 942016.

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Quantitative Determination of Brain Analytes

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Male C57BL/6J mice were treated with an oral dose of 10.0 mg/kg of 4a or 5f for 1 h before euthanized and perfused with 0.9% normal saline. The brains were quickly removed and homogenized in 3 volumes 0.9% normal saline on ice. After centrifuged for 10 min at 4 °C, the supernatants were collected and pretreated with Methanol before measured by LC‒MS/MS (Thermo, TSQ QUANTUM ACCESS MAX, USA). Shim-pack GIST C18-AQ column (2.1 mm × 50 mm I.D., 5 μm, Shimadzu) were used for the chromatographic separation, mobile phase = MeOH (100%), flow rate = 250 μL/min, and sample injection volume = 10 μL. The analysis detection conditions were as follows: electrospray ionization (ESI), positive ion mode, the spray voltage was 3500 V and sheath gas flow rate was 50 arb, the vaporizer temperature was 300 °C, and the capillary temperature was 350 °C.

Zhou Q., Le M., Yang Y., Wang W., Huang Y., Wang Q., Tian Y., Jiang M., Rao Y., Luo H.B, & Wu Y. (2022). Discovery of novel phosphodiesterase-1 inhibitors for curing vascular dementia: Suppression of neuroinflammation by blocking NF-κB transcription regulation and activating cAMP/CREB axis. Acta Pharmaceutica Sinica. B, 13(3), 1180-1191.

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UPLC-MS/MS Analysis of Barbiturates

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Analysis of barbiturates was performed using an Ultimate 3000 UPLC system interfaced with a TSQ Quantum Access MAX triple quadrupole Mass Spectrometry (Thermo Scientific, Waltham, MA, USA). Chromeleon client software (Thermo Scientific, Waltham, MA, USA) was used for LC control, and Xcalibur software (Thermo Scientific, Waltham, MA, USA) was used to control the MS, data acquisition, and data processing. Chromatographic separation was conducted at 45 °C on an Accucore C18 column (2.1 mm × 100 mm, 2.6 μm) (Thermo Scientific, Waltham, MA, USA) with gradient elution. Water containing 0.5% of acetic acid (v/v) was used as mobile phase A, and methanol was used as mobile phase B. Mobile phase B started from 20% for 0.5 min, and then increased to 95% in 1.5 min and 95% was kept for 2 min. At the end, mobile phase B was decreased to 20% within 0.1 min, which was kept for 1.9 min for equilibration. The flow rate was set to 0.4 mL min⁻¹ and the injection volume was 10 μL. Mass spectrometry was performed with an ESI source in the negative-ionization mode with a sheath gas of 40 Arb and aux gas of 10 Arb. The capillary temperature was set at 320 °C and the vaporizer temperature was set at 350 °C. The spray voltage was 3.2 kV. The parameters for the quantification selected reaction monitoring (SRM) transitions are presented in Table 4.

Zhu R., Dong Y., Cai X, & Huang C. (2019). Determination of Barbiturates in Biological Specimens by Flat Membrane-Based Liquid-Phase Microextraction and Liquid Chromatography-Mass Spectrometry. Molecules, 24(8), 1494.

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UPLC-MS/MS Analytical Protocol for Compound Quantification

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The UPLC system (Thermo Fisher Scientific) included an Ultimate 3000 RSLC system
with binary pumps, a WPS-3000TRS autosampler, and a TCC-3000RS column oven. The
chromatographic separation was performed using an Ultimate XB-C₁₈column (2.1 mm × 50 mm, 1.8 μm; Welch Materials, Shanghai, China). The binary
mobile phase system consisted of 0.1% formic acid in water (A) and 0.1% formic
acid in acetonitrile (B). The gradient program was as follows: 0 to 3.5 minutes,
5% to 95% B; 3.5 to 4.5 minutes, 95% B; 4.5 to 5.0 minutes, 95% to 5% B; and 5.0
to 6.0 minutes, 5% B. The flow rate was 0.4 mL·minute⁻¹. The column
temperature was 45°C.
The MS analysis was performed on a TSQ Quantum Access MAX (Thermo Fisher
Scientific) equipped with electrospray ionization. The compounds were ionized in
the positive and negative ion modes. The optimized parameters of the MS analysis
were set as follows: spray voltage, 4000 V (positive and negative); capillary
temperature, 350°C; sheath gas (nitrogen) pressure, 40 arb; and aux gas
(nitrogen) pressure, 15 arb. Argon was used as the collision gas. Quantification
was performed using the selected reaction monitoring (SRM) mode. The SRM
transitions and conditions for measurement of the compounds are summarized in
Table 1. Data
were acquired using ThermoXcalibur software (version 3.0, Thermo Fisher
Scientific).

Gu Y., Piao X, & Zhu D. (2020). Simultaneous determination of calycosin, prim-O-glucosylcimifugin, and paeoniflorin in rat plasma by HPLC-MS/MS: application in the pharmacokinetic analysis of HQCF. The Journal of International Medical Research, 48(11), 0300060520972902.

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Purine Metabolite Identification by Mass Spectrometry

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Purine metabolites were identified by mass and retention time with a triple quadrupole mass spectrometer (TSQ Quantum Access Max, Thermo) with heated electrospray ionization (HESI). The HESI source was operated in negative-ion mode with the following settings: spray voltage 4000 V, vaporizer temperature 250°C, sheath gas pressure 50 arb, ion sweep gas pressure 0.0 arb, auxillary gas pressure 10 arb, and capillary temperature 300°C. Compounds were identified by comparing the retention time and selected reaction monitoring (SRM) pairs (Table 3) during one scan event with a 20 msec scan time, as well as through the addition of purine standards. Data was processed using computer software (Xcalibur Quan Browser, Thermo).

Ardente A., Garrett T., Wells R., Walsh M., Smith C., Colee J, & Hill R. (2016). A Targeted Metabolomics Assay to Measure Eight Purines in the Diet of Common Bottlenose Dolphins, Tursiops truncatus. Journal of chromatography & separation techniques, 7(5), 334.

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Analytical Techniques for Drugs of Abuse Detection

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Routine analyses for the common drugs of abuse and prescription drugs involved the use of enzyme-linked immunosorbent assay, high-performance liquid chromatography coupled with diode array detection and liquid chromatography with mass spectrometry. New psychoactive substances (NPS), including SCs, were identified and quantified by liquid chromatography coupled with a triple quadrupole mass spectrometer—Thermo Scientific TSQ Quantum Access MAX. The analytes were separated in a Thermo Scientific C18 column (150 × 2.1 mm ID, 5 μm). The mobile phase was fed into the column in gradient mode in the form of a mixture of 0.2% formic acid solution in water (v/v) and 0.2% formic acid solution in acetonitrile (v/v). The mass spectrometer was operated in the positive ion detection mode using full scan acquisition and multiple reaction monitoring. The analytes were isolated from the matrix by acetonitrile precipitation as well as extraction with ethyl acetate. The exact test procedure was published together with the detailed description of two cases of intoxication with the PV8 SC (8 (link)).

Pieprzyca E., Skowronek R, & Czekaj P. (2021). Toxicological Analysis of Intoxications with Synthetic Cathinones. Journal of Analytical Toxicology, 46(7), 705-711.

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Polyphenol Identification by LC-MS

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The polyphenols were identified by liquid chromatography-mass spectrometry (LC-MS) (TSQ Quantum Access MAX, Thermo Fisher Scientific, USA) using C18 as a separation column (5 μm 120 Å 4.6 × 250 mm, Thermo Scientific, U.S.) (Alu'Datt et al., 2013 (link)). Using 100% methanol and 0.2% as mobile phases, the separation protocol was as follows: 0–50 min (from 5% methanol and 95% acetic acid to 80% methanol and 20% acetic acid) and 50–60 min (from 80% methanol and 20% acetic acid to 5% methanol and 95% acetic acid), with a flow rate of 0.75 mL min⁻¹. The sample injection volume was 20 μL and the column temperature was 25 °C, and the fractions were determined at a wavelength of 280 nm. Based on the results, polyphenols were identified using The Human Metabolome Database (HMDB) (https://hmdb.ca/).

Lin Z., Wei H., Zhang Y., Liu P., Liu Y., Huang Z., Lv X., Zhang Y, & Zhang C. (2022). Improving emulsification properties of alkaline protein extract from green tea residue by enzymatic methods. Current Research in Food Science, 5, 1235-1242.

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Rapid Purification and Identification of Lutein from Microalgae

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The preparative purification was performed using Thermo Fisher Scientific Ultimate 3000 HPLC system with a YMC-C₃₀ semiprep Carotenoid column (10 × 250 mm, 5μm). The mobile phase system was a binary gradient system with at a flow rate of 4.0 mL/min at 446 nm with a column temperature of 25 °C and the injection volume was 2.0 mL. The gradient mobile phase was as followed: the collected fractions were then combined and separated into two before evaporation under nitrogen gas. Each was dissolved in methanol for further LC-MS/MS analysis.
In the present study, the isolated lutein obtained from S. obliquus extract was confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) equipped with an atmospheric pressure chemical ionization probe (APCI). The mass spectrometer (Thermo Scientific/TSQ Quantum Access Max) was operated in full scan mode from m/z 50–900 at 350 °C for vaporization temperature.

ERDOĞAN A., KARATAŞ A.B., DEMİREL Z, & CONK DALAY M. (2022). Induction of lutein production in Scenedesmus obliquus under different culture conditions prior to its semipreparative isolation. Turkish Journal of Chemistry, 46(3), 796-804.

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