Mcf 10a special medium

Human BC cell line MDA‐MB‐231, human breast epithelial cell line MCF‐10A and human leukemic cell line THP‐1 were all acquired from American Type Culture Collection (ATCC). MDA‐MB‐231 and THP‐1 cells were cultivated in RPMI‐1640 medium containing 10% FBS. MCF‐10A cell was cultivated in MCF‐10A special medium (Procell). All cells were incubated over a humidified 5% CO₂ atmosphere with 95% air at 37°C. THP‐1 cells (1 × 10⁶) were supplemented with 100 ng/mL PMA (Sigma‐Aldrich) for 24 h to stimulate differentiation into M0 macrophages. As for exosome isolation, MDA‐MB‐231 and MCF‐10A cells were supplemented with exosome‐depleted FBS medium (SBI).

The human normal mammary epithelial cell line, MCF10A (cat no. CL-0525), was purchased from Wuhan Procell Company and cultured in MCF10A special medium (Procell Co., Ltd.). The human BC cell lines, SKBR3 (cat no. 1101HUM-PUMC000085), MCF7 (cat no: 1101HUM-PUMC000013), MDA-MB-231 (cat no. 1101HUM-PUMC000014) and Hs578T (cat no. 1101HUM-PUMC000670) and MDA-MB-468 (cat no. 1101HUM-PUMC000249), were purchased from the National Infrastructure of Cell line Resource (NICR). The SKBR3, MCF7, MDA-MB-231 and Hs578T cells were cultured in DMEM (Meilun Biotech Co., Ltd.) and the MDA-MB-468 cells were cultured in RPMI-1640 (Meilun Biotech Co., Ltd.) medium supplemented with 10% fetal bovine serum (HyClone; Cytiva), 100 U/ml penicillin and 100 µg/ml streptomycin (Gibco; Thermo Fisher Scientific, Inc.) in a 5% CO₂ incubator at 37°C.