Smz800 microscope

Manufactured by Nikon

Sourced in Japan

The Nikon SMZ800 is a stereomicroscope designed for a variety of applications. It features a zoom range of 0.8x to 8x, providing a wide range of magnification options. The microscope is equipped with an LED illumination system and offers adjustable brightness control. The SMZ800 is compatible with a range of accessories to further enhance its functionality.

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Lab products found in correlation

Nis elements software, by Nikon (3 mentions) Nis element, by Nikon (2 mentions) Eclipse te2000 s, by Nikon (2 mentions) Ds fi1, by Nikon (2 mentions) Methylene blue, by Merck Group (1 mentions) Epofix, by Electron Microscopy Sciences (1 mentions) Szx16 fluorescent microscope, by Olympus (1 mentions) Vegf a165, by Thermo Fisher Scientific (1 mentions) Digital sight ds u3 camera, by Nikon (1 mentions) Western blue, by Promega (1 mentions) Eclipse 80i microscope, by Nikon (1 mentions) Spot insight camera, by Nikon (1 mentions) Eos 5d mark 3, by Canon (1 mentions) Mp e 65 mm, by Canon (1 mentions) Mp e65mm f2.8 1 5 macro photo lens, by Canon (1 mentions) Eos 7d, by Olympus (1 mentions) Xl30 scanning electron microscope, by Philips (1 mentions) Digital sight ds l2, by Nikon (1 mentions) Metamorph software, by Universal Imaging (1 mentions) Tds 1002, by Tektronix (1 mentions)

29 protocols using smz800 microscope

GUS Staining of BKN1 and BKN2 Promoter Plants

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Inflorescences or stage 12 flowers from the BKN1 and BKN2 promoter-GUS transgenic plants were incubated in GUS solution overnight at 37 °C according to the protocol used in Wang et al. [15 (link)]. Tissues were then fixed in ethanol:glacial acetic acid and cleared with chloral hydrate solution as described by [105 ]. Tissues were mounted in 30% glycerol and images were taken on a Nikon sMz800 microscope.

Doucet J., Lee H.K., Udugama N., Xu J., Qi B, & Goring D.R. (2019). Investigations into a putative role for the novel BRASSIKIN pseudokinases in compatible pollen-stigma interactions in Arabidopsis thaliana. BMC Plant Biology, 19, 549.

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Characterization of Pickering Emulsions

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SEM-EDX was performed using a Quanta FEG 250 at an acceleration voltage of 30 kV under high vacuum (8 × 10⁻⁵ mbar). The SEM samples were prepared by adding 100–200 µL of ethanol into the Pickering emulsion samples to destabilize the emulsions and form interfacial arrays which were dried onto aluminum foil in a vacuum oven. Optical microscopy of the Pickering emulsions was performed using a Nikon SMZ800 microscope. TEM images were recorded using a Joel JEM-1400 Plus Transmission Electron Microscope at an acceleration voltage 80 kV. The TEM samples were fabricated by extracting a Pickering emulsion droplet with a carbon film covered TEM grid (S160,200 mesh Cu (25)), then drying at room temperature.

Zhang Y., Ye Z., Li C., Chen Q., Aljuhani W., Huang Y., Xu X., Wu C., Bell S.E, & Xu Y. (2023). General approach to surface-accessible plasmonic Pickering emulsions for SERS sensing and interfacial catalysis. Nature Communications, 14, 1392.

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Quantifying MSC Attachment on PES/PVP Membranes

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MSC attachment on flat porous PES/PVP membranes uncoated or coated with fibronectin (200 µg/mL) was assessed, as described in Skrzypek et al. [48 ]. In short: MSCs were seeded with a density of 10.000 cells/cm² and kept in culture for 1 day. Subsequently, samples were fixed in 10% buffered formalin for 10 min Subsequently, samples were fixed in 10% buffered formalin for 10 min After fixation samples were washed in dH2O (2x) and stained with methylene blue (Sigma-Aldrich) for 10 sec. followed by a 3x wash with dH2O. Quantification of the number of cells on the membranes was done by taking 3 pictures of each membrane and counting the number of cells (Nikon SMZ800 microscope).

Groot Nibbelink M., Skrzypek K., Karbaat L., Both S., Plass J., Klomphaar B., van Lente J., Henke S., Karperien M., Stamatialis D, & van Apeldoorn A. (2018). An important step towards a prevascularized islet microencapsulation device: in vivo prevascularization by combination of mesenchymal stem cells on micropatterned membranes. Journal of Materials Science. Materials in Medicine, 29(11), 174.

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Quantifying Deciduous Tooth Root Resorption

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Extracted deciduous teeth were stored in 5% buffered formaldehyde. Before embedding, the teeth were washed several times in 70% ethanol, with a final wash in absolute ethanol. Then, the teeth were embedded in epoxy-resin (Epofix®; Electron Microscopy Sciences, Fort Washington, PA), and each tooth was cut sagittally, in a bucco-lingual direction into halves in a Leitz low-speed saw microtome. From the central part of the teeth, three sections were cut with a thickness of 110 μm. All sections were photographed with fiber-optic surface illumination in a Nikon SMZ800 microscope equipped with a Nikon Digital Sight DS-F1 camera. The slanted root resorption evident on the most central cut of the tooth was used as gold standard (Fig. 2).Fig. 2

Microscopic image of an extracted deciduous canine showing slanted root resorption and how the root length measurements from cemento-enamel junction (CEJ) to the most resorbed (MR) side and from CEJ to the less resorbed (LR) root side were measured

Alamadi E., Alhazmi H., Hansen K., Lundgren T, & Naoumova J. (2017). A comparative study of cone beam computed tomography and conventional radiography in diagnosing the extent of root resorptions. Progress in Orthodontics, 18, 37.

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Escape response and heartbeat analysis in larval zebrafish

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By gently touching the caudal fin with a pipette tip, the escape or startle response of 16 larvae in a 96 well plate was examined and manually recorded using an Olympus SZX16 fluorescent microscope. This locomotor response was classified into normal, reduced or absent
^{16 (link)}. For heartbeat analysis, larvae were placed into methylcellulose followed by a 10-second desensitization period. Heart rate was observed using a Nikon SMZ800 microscope and manually counted for 30 seconds. The number of beats was multiplied by 2 to calculate the heart rate per minute.

Gómez Sánchez A., Colucci P., Moran A., Moya López A., Colligris B., Álvarez Y, & Kennedy B.N. (2023). Systemic treatment with cigarette smoke extract affects zebrafish visual behaviour, intraocular vasculature morphology and outer segment phagocytosis. Open Research Europe, 3, 48.

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Aortic Ring Angiogenesis Assay

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Aortae were isolated from mice (8 weeks old) and cut into ~25 rings each. Aortic rings were embedded in matrigel (356234, BD Biosciences, Franklin Lakes, NJ), and stimulated with 30 ng/ml VEGF-A₁₆₅(450–32, Peprotech). Images were taken after 24 hr with a Nikon SMZ800 microscope.

Tetzlaff F., Adam M.G., Feldner A., Moll I., Menuchin A., Rodriguez-Vita J., Sprinzak D, & Fischer A. (2018). MPDZ promotes DLL4-induced Notch signaling during angiogenesis. eLife, 7, e32860.

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Quantitative Kidney Histology Imaging

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Periodic acid-Schiff-Hematoxylin stained kidney sections were imaged using either a Nikon Eclipse TE2000-S or a Nikon SMZ800 microscope. Images were acquired and background-corrected using Nikon NIS Elements software (Version 4.60) and all images were color-adjusted in an identical fashion in open source ImageJ software (Version 2.0.0).

Lever J.M., Yang Z., Boddu R., Adedoyin O., Guo L., Joseph R., Traylor A.M., Agarwal A, & George J.F. (2017). Parabiosis Reveals Leukocyte Dynamics in the Kidney. Laboratory investigation; a journal of technical methods and pathology, 98(3), 391-402.

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Quantifying Periodontal Bone Heights

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Periodontal bone heights (i.e., the distances from the cement–enamel junction [CEJ] to the alveolar bone crest [ABC]) were measured in defleshed maxillae under a Nikon SMZ800 microscope using a 40x objective. Briefly, images of the maxillae were captured using a Nikon Digital Sight DS-U3 camera controller and CEJ–ABC distances were measured at 14 predetermined sites [41 (link)] or, in the case of split-mouth experiments, at 6 predetermined sites [42 (link)] using NIS-Elements software (Nikon Instruments). For calculating change in bone levels (e.g., relative bone loss in CD18^−/− mice versus WT controls), the 14-site (or 6-site) total CEJ-ABC distance for each mouse was subtracted from the mean CEJ-ABC distance of control mice. The results are presented in mm; negative values indicate bone loss and positive values indicate bone gain relative to controls. In experiments involving split-mouth design (treatments with drug vs. control in opposite halves of the maxilla), the data shown involve measurements for each hemi-maxilla.

Kajikawa T., Wang B., Li X., Wang H., Chavakis T., Moutsopoulos N.M, & Hajishengallis G. (2020). Activation of metabolic nuclear receptors restores periodontal tissue homeostasis in mice with leukocyte adhesion deficiency-1. Journal of leukocyte biology, 108(5), 1501-1514.

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Immunolocalization of PtFLA6 in Bent Stems

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Segments (0.5 cm) of the bended stems for two weeks were fixed overnight in 4% paraformaldehyde in 0.1 M phosphate-buffered saline (PBS, pH 7.5). Sections (10 µm) were cut out and blocked with 5% bovine serum albumin (BSA) for 1 h at room temperature. Then the sections were incubated with the PtFLA6 antibody (diluted at 1:50 with 0.1 M PBS containing 0.1% BSA) at room temperature for 1 h. After rinsed three times in PBS (5 min each), the samples were immersed in the alkaline phosphatase-conjugate secondary antibody (diluted at 1:100) for 1 h and then stained with 150–200 µl of Western Blue (Promega, USA). When blue color appeared, images were captured under bright field using the ECLIPSE 80i microscope (Nikon, Japan) or SMZ800 microscope (Nikon, Japan). Pre-immune serum was used as the negative control.

Wang H., Jin Y., Wang C., Li B., Jiang C., Sun Z., Zhang Z., Kong F, & Zhang H. (2017). Fasciclin-like arabinogalactan proteins, PtFLAs, play important roles in GA-mediated tension wood formation in Populus. Scientific Reports, 7, 6182.

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Quantitative Myocardial Infarct Evaluation

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Paraffinized hearts were transversely sectioned, in entirety, from apex to base, after which eight 4 µm-thick sections from equidistant (∼0.8 mm) intervals were placed on microscope slides. The slides were stained with Masson trichrome to quantitatively assess scar size (Leach et al., 2017 (link)). Briefly, trichrome-stained sections were examined with a Nikon SMZ800 microscope and photographed at 10× magnification using a SPOT Insight camera (Nikon Instruments). MIQuant software was used to quantitate infarct size in sections between the apex and the ligation suture site, as previously described (Leach et al., 2017 (link)). Results were expressed as the average percentage of area and midline length around the left ventricle.

Wang X., Lauth A., Wan T.C., Lough J.W, & Auchampach J.A. (2020). Myh6-driven Cre recombinase activates the DNA damage response and the cell cycle in the myocardium in the absence of loxP sites. Disease Models & Mechanisms, 13(12), dmm046375.

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