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8 protocols using silver nitrate agno3

1

Silver Nanoparticle Characterization

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Ag NPs (20 and 200 nm) were obtained from PlasmaChem GmbH (Berlin, Germany). Silver nitrate (AgNO3) was purchased from VWR (Leuven, Belgium) and Alcian blue, H2O2, Toluidine blue, 2′,7′-dichlorofluorescin diacetate (DCFH-DA), osmium tetroxide (OsO4), glutaraldehyde, resazurin sodium salt and the epoxy resin embedding kit from Sigma Aldrich (Bornem, Belgium). IL-8 kit was obtained from ENZO Life Sciences BVBA (Zandhoven, Belgium).
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2

Photopolymer Resin and Metal Salt Synthesis

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Clear photopolymer standard resin (a mixture of proprietary acrylic monomers and oligomers and phenylbis (2,4,6-trimethyl benzoyl)-phosphine oxide as a photoinitiator) was purchased from XYZprinting, Inc (XYZprinting, New Taipei City, Taiwan). Silver nitrate (AgNO3) was purchased from VWR Chemicals, silver perchlorate (AgClO4) was purchased from Alfa Aesar, and isopropanol (IPA) was purchased from Scharlau. All products were used as received.
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3

Radiolabeled Gold Nanoparticle Synthesis

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Gold(III) chloride trihydrate (HAuCl4 · 3H2O), cetyltrimethylammonium bromide (CTAB), sodium borohydride (NaBH4), l-ascorbic acid (Vitamin C), diethylenetriaminepentaacetic dianhydride (DTPA anhydride), Ninhydrin reagent (2% solution), sodium carboxymethyl cellulose (CMC, Mw = ∼ 700,000) and Tween® 20 were purchased from Sigma-Aldrich (UK). Silver nitrate (AgNO3) was supplied by VWR International (UK). NH2-PEG-SH (amine-PEG3500-thiol, average Mw = 3500) was purchased from JenKem Technology (USA). Sulfo-cyanine5 NHS ester was purchased from Lumiprobe (USA). Nitric acid (HNO3, 68%, w/w) and hydrochloric acid (HCl, 37%, w/w) with trace metal grades for ICP-MS measurements were obtained from Thermo Fisher Scientific (UK). Indium-111 chloride was purchased from Curium (The Netherlands). Isoflurane (IsoFlo®) for anaesthesia was purchased from Zoetis (UK). Dialysis bags (SnakeSkin Dialysis Tubing, MWCO = 3500) were purchased from Thermo Fisher Scientific (UK). PD-10 columns and NAP™-5 columns were purchased from GE Healthcare Life Sciences (UK). Thin-layer chromatography (TLC) silica gel on aluminium sheets was purchased from Merck Millipore (UK). Instant TLC papers (iTLC-SG) were purchased from Agilent Technologies (UK). Other chemicals, not specified here, were all analytical grades purchased from standard chemical suppliers and used without further purification.
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4

Synthesis of Silver Nanoparticles via Hydrothermal Reduction

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All reagents were of analytical grade and solutions were prepared using ultrapure water (Millipore MilliQ system, TKA Germany). Silver nitrate (AgNO3) and N-methyl-2-pyrrolidone (NMP, 99.9 wt%) were obtained from VWR Chemicals (Leuven, Belgium). Poly(acrylic acid) (PAA, Mw = 1800), hydrazine hydrate (N2H4; 50–60 wt%), ethylene glycol (EG; anhydrous, 99.8 wt%) and 3-morpholynopropylamine (MPA) were purchased from Sigma-Aldrich (Burlington, MA, USA). Terpineol (TpOH; mixed isomers, 98 wt%) was purchased from Alfa Aesar (Kandel, Germany). Ethanol (EtOH, 96 wt%), isopropyl alcohol (IPA; anhydrous, 99.5 wt%) and citric acid were obtained from Gram-Mol (Zagreb, Croatia). N,N′-diisopropylcarbodiimide (DCI) was bought from Merck (Darmstadt, Germany) and sodium hydroxide from Kemika (Zagreb, Croatia).
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5

Polyclonal Anti-Haptoglobin Immunoassay Protocol

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Polyclonal mouse anti-Hp, haptoglobin (Hp) from human plasma, bovine serum albumin (BSA), tween-20, sodium chloride (NaCl), and hydrogen peroxide (H2O2) solution were obtained from Sigma. Sodium carbonate (Na2CO3), L-ascorbic acid (C6H8O6), and glacial acetic acid (C2H4O2) were all obtained from BDH chemicals. Sodium acetate (NaAc), and silver nitrate (AgNO3) were obtained from VWR chemicals. Tris-HCl was obtained from 1st base. Polyethylene glycol (PEG)-20000 was obtained from Wako Chemicals. K-Blue ® Aqueous 3,3′,5,5′tetramethylbenzidine (TMB) substrate was obtained from Neogen and was used as is. Gold nanoparticle (AuNP) stabilized in citrate was obtained from BBI solution.
The buffers used in the experiment are as follows: (1) TBS buffer, (2) conjugating buffer, containing 0.2 M Na2CO3, pH 9.8, (3) washing buffer TBST, (4) blocking buffer for conjugation, PBS buffer containing 10% BSA (w/v), ( 5) blocking buffer for immunoassay, TBS buffer containing 1% PEG (w/v), ( 6) acetate buffer, 0.2 M, pH 3 adjusted using acetic acid.
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6

Brewery Waste-Facilitated Silver Nanoparticles

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Silver nitrate (AgNO3) was purchased from Avantor Performance Materials Poland S.A. company (former POCH S.A., Gliwice, Poland). Brewery waste was obtained from Jabłonowo Brewery, Wólka Kosowska, Poland S.A. The strong light beer of Jabłonowo Brewery was purchased from a commercial outlet. Brewery waste from stage 9 of the production line (BW9) or the final brewery product (B) was used as a precursor, and silver nitrate was the metal salt precursor.
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7

Synthesis and Characterization of Ga-HA and Ag-HA Powders

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Hydroxyapatite powders enriched with gallium (Ga-HA) or silver ions (Ag-HA) with 0.45 wt% and 0.54 wt% nominal value of Ga or Ag, respectively, were synthesized using the conventional wet method (coprecipitation in an aqueous solution), which was described in detail in our previous work [57 (link)]. The following reagents were used in the aforementioned synthesis: calcium nitrate tetrahydrate Ca(NO3)2·4H2O (Sigma-Aldrich, Bangalore, India), ammonium dibasic phosphate (NH4)2HPO4 (Chempur, Piekary Śląskie, Poland), silver nitrate AgNO3 (Avantor Performance Materials, Gliwice, Poland) and gallium nitrate trihydrate Ga(NO3)3·3H2O (Sigma-Aldrich, Burlington, MA, USA) as sources of calcium, phosphorus, silver and gallium, respectively. Briefly, an aqueous solution of (NH4)2HPO4 was added dropwise into an aqueous solution of Ca(NO3)2·4H2O and one of the aforementioned reagents (AgNO3 or Ga(NO3)3 3H2O) and stirred gently at pH 10 and at a temperature of 60 °C for 2 h. Then, the obtained precipitate was left for 24 h for ageing. Next, the precipitates were filtered, soaked several times in distilled water and dried at 120 °C in air. For comparison, pure, unsubstituted hydroxyapatite (HA) was synthesized by the same method.
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8

Fabrication of EVOH/EPC Biopolymer Composites

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The ethylene–vinyl alcohol (EVOH), commercial grade F101B, with an ethylene content of 32%, was obtained from Kuraray (Japan). The ethylene-propylene copolymers (EPC) commercial grade Vistamaxx™2120 based olefinic elastomer is produced using Exxon Mobil Chemical’s Exxpol™ technology (Spring, TX, USA).
Glycerol (C3H8O3), sodium stearate (C18H35NaO2) and silver nitrate (AgNO3) were supplied by Avantor Performance Materials Poland S.A., and used directly without further purification.
Some of the properties of EVOH and EPC are listed in Table 1.
The chemical structure of components is shown in Figure 1.
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