Guanosine diphosphate gdp

Manufactured by Merck Group

Sourced in United States

Guanosine diphosphate (GDP) is a nucleotide molecule that plays a crucial role in cellular processes. It serves as a substrate for various enzymes and is involved in the regulation of cellular activities. GDP is a key component in the synthesis and metabolism of nucleic acids and other biomolecules.

Automatically generated - may contain errors

Lab products found in correlation

35s gtpγs, by PerkinElmer (6 mentions) Damgo, by Merck Group (6 mentions) 3h damgo, by PerkinElmer (4 mentions) Cell culture media and supplements, by Merck Group (4 mentions) 3h diprenorphine, by PerkinElmer (3 mentions) Diprenorphine, by Merck Group (3 mentions) Unlabeled gtpγs, by Merck Group (2 mentions) 3h nociceptin, by PerkinElmer (2 mentions) U69 593, by Merck Group (2 mentions) Tris hydroxymethyl aminomethane tris, by Merck Group (2 mentions) Naloxone, by Merck Group (2 mentions) Hank s balanced salt solution hbss, by Merck Group (2 mentions) Forskolin, by Merck Group (2 mentions) D pen2 d pen5 enkephalin u69 593, by Merck Group (2 mentions) 35s gtpγs 1250 ci mmol, by PerkinElmer (1 mentions) Naloxone, by Thermo Fisher Scientific (1 mentions) 3h mazindol, by PerkinElmer (1 mentions) U50 488, by Thermo Fisher Scientific (1 mentions) 3h dtg, by PerkinElmer (1 mentions) Naloxone methiodide, by Merck Group (1 mentions)

8 protocols using guanosine diphosphate gdp

Opioid Receptor Binding Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell culture media and supplements were obtained from Sigma-Aldrich Chemicals (St. Louis, MO, USA). Radioligands [³H]DAMGO (50 Ci/mmol) and [³⁵S]GTPγS (1250 Ci/mmol) were purchased from PerkinElmer (Boston, MA, USA). DAMGO, unlabeled GTPγS, and guanosine diphosphate (GDP) were obtained from Sigma-Aldrich Chemicals (St. Louis, MO, USA). All other chemicals were of analytical grade and obtained from standard commercial sources.

Dumitrascuta M., Bermudez M., Ballet S., Wolber G, & Spetea M. (2020). Mechanistic Understanding of Peptide Analogues, DALDA, [Dmt1]DALDA, and KGOP01, Binding to the Mu Opioid Receptor. Molecules, 25(9), 2087.

+ Open protocol

+ Expand

Subcutaneous Nicotine and Morphine Effects

Check if the same lab product or an alternative is used in the 5 most similar protocols

(−)-nicotine hydrogen tartrate salt [(−)-1-methyl-2-(3-pyridyl) pyrrolidine (+)-bitartrate salt] was purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA); morphine, [d-Ala2,(NMe)Phe4,Gly5-OH] enkephalin (DAMGO), and naloxone were supplied by the National Institute on Drug Abuse (Rockville, MD). All drugs were dissolved in sterile saline (0.9% sodium chloride) and prepared fresh before each experiment. Nicotine, morphine and saline were injected subcutaneously (s.c.) and naloxone was injected intraperitoneally (i.p.). Control groups received saline injections at the same volume and by the same route of administration. All doses are expressed as the free base of the drug. [³⁵S]GTPγS (1250 Ci/mmol) was purchased from PerkinElmer Life Sciences (Boston, MA). Guanosine diphosphate (GDP) was purchased from Sigma-Aldrich (St. Louis, MO). Econosafe scintillation fluid was purchased from Research Products International. All other reagent grade chemicals were obtained from Sigma Chemical Co. (St. Louis MO) or Fisher Scientific (Pittsburgh PA).

Kota D., Alajaji M., Bagdas D., Selley D.E., Sim-Selley L.J, & Damaj M.I. (2018). Early adolescent nicotine exposure affects later-life hippocampal mu-opioid receptors activity and morphine reward but not physical dependence in male mice. Pharmacology, biochemistry, and behavior, 173, 58-64.

+ Open protocol

+ Expand

Radioligand Binding Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

³H-Diprenorphine (#NET1121250UC), ³H-Nociceptin (#NET1130050UC), ³H-CP55,940 (#NET1051025UC), ³H-Mazindol (#NET816250UC), ³H-DTG (#NET986250UC), and ³⁵S-GTPγS (#NEG030H250UC) were all obtained from PerkinElmer. Guanosine diphosphate (GDP) was obtained from Sigma Aldrich (St. Louis, MO), stored at -20°C under desiccation, made fresh for each experiment, and discarded after 60 days. All clinical opioid drugs were obtained from Depomed, Inc. (Newark, CA; authors on this manuscript). Naloxone, nociceptin, BD1008, S-duloxetine, U50,488, and GBR12909 were obtained from Fisher Scientific (distributed from Tocris, R&D). WIN55,212 was obtained from Fisher Scientific (distributed from Cayman Chemical). Endomorphin-2 and DPDPE were synthesized using a standard solid phase peptide synthesis protocol, and validated for purity (>95%) by HPLC and identity by mass spectrometry. All compound powders were stored as recommended by the manufacturer. 10 mM drug stock solutions were made in vehicle and stored at -20°C for no more than 30 days. Standard chemicals and buffers were purchased from Fisher Scientific with a minimum purity of 95%.

Olson K.M., Duron D.I., Womer D., Fell R, & Streicher J.M. (2019). Comprehensive molecular pharmacology screening reveals potential new receptor interactions for clinically relevant opioids. PLoS ONE, 14(6), e0217371.

+ Open protocol

+ Expand

Radioligand Binding and G-Protein Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell culture media
and supplements were obtained from Sigma-Aldrich Chemicals (St. Louis,
MO) or Life Technologies (Carlsbad, CA). Radioligands [³H]DAMGO, [³H]diprenorphine, [³H]U69,593, and
[³⁵S]GTPγS were purchased from PerkinElmer (Boston).
[³H][Ile^5,6]deltorphin II was obtained from
the Institute of Isotopes Co. Ltd. (Budapest, Hungary). DAMGO, [d-Pen²,d-Pen⁵]enkephalin (DPDPE),
diprenorphine, U69,593, naloxone, naloxone methiodide, tris(hydroxymethyl)
aminomethane (Tris), 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic
acid (HEPES), unlabeled GTPγS, and guanosine diphosphate (GDP)
were obtained from Sigma-Aldrich Chemicals (St. Louis, MO). Morphine
hydrochloride was obtained from Gatt-Koller GmbH (Innsbruck, Austria).
All other chemicals were of analytical grade and obtained from standard
commercial sources. Test compounds were prepared as 1 mM stocks in
water and further diluted to working concentrations in the appropriate
medium.

Spetea M., Rief S.B., Haddou T.B., Fink M., Kristeva E., Mittendorfer H., Haas S., Hummer N., Follia V., Guerrieri E., Asim M.F., Sturm S, & Schmidhammer H. (2018). Synthesis, Biological, and Structural Explorations of New Zwitterionic Derivatives of 14-O-Methyloxymorphone, as Potent μ/δ Opioid Agonists and Peripherally Selective Antinociceptives. Journal of Medicinal Chemistry, 62(2), 641-653.

+ Open protocol

+ Expand

Opioid Receptor Binding Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

[³H]DAMGO and [³⁵S]GTPγS were purchased from PerkinElmer (Boston, USA). DAMGO, naloxone, dimethylsulfoxide (DMSO), tris(hydroxymethyl)aminomethane (Tris), 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES), GTPγS, guanosine diphosphate (GDP), forskolin and Hanks’ balanced salt solution (HBSS) were obtained from Sigma-Aldrich Chemicals (St. Louis, MO). Morphine hydrochloride was obtained from Gatt-Koller GmbH (Innsbruck, Austria). All cell culture media and supplements were from Sigma-Aldrich Chemicals (St. Louis, MO) and Invitrogen (Paisley, UK). All other chemicals were of analytical grade, and were obtained from standard commercial sources. The selected compounds for biological testing were obtained from Maybridge (www.Maybridge.com), and their purities were all higher than 95%.

Kaserer T., Lantero A., Schmidhammer H., Spetea M, & Schuster D. (2016). μ Opioid receptor: novel antagonists and structural modeling. Scientific Reports, 6, 21548.

+ Open protocol

+ Expand

Radioligand Binding Assay Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

LENART01 was prepared as described previously [59 (link)]. Radioligands [³H]DAMGO (51.7 Ci/mmol), [³H]DPDPE (47.4 Ci/mmol), [³H]U69,593 (49.3 Ci/mmol), [³H]nociceptin (119.4 Ci/mmol) and [³⁵S]GTPγS (1250 Ci/mmol) were purchased from PerkinElmer (Boston, MA, USA). Guanosine diphosphate (GDP), GTPγS, DAMGO, DPDPE, U69,593, nociceptin, formalin, polyethylenimine (PEI), tris(hydroxymethyl) aminomethane (Tris), 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES), bovine serum albumin (BSA) and cell culture media and supplements were obtained from Sigma-Aldrich Chemicals (St. Louis, MO, USA). Dermorphin was obtained from MCE (Sollentuna, Sweden). Naloxone hydrochloride was kindly provided by Dr. Helmut Schmidhammer (University of Innsbruck, Innsbruck, Austria). All other chemicals were of analytical grade and obtained from standard commercial sources. Test compounds were prepared as 1 mM stocks in water for in vitro assays or dissolved in 0.9% physiological saline for in vivo assays and further diluted to working concentrations in the appropriate medium.

Hochrainer N., Serafin P., D’Ingiullo S., Mollica A., Granica S., Brytan M., Kleczkowska P, & Spetea M. (2024). In Vitro and In Vivo Pharmacological Profiles of LENART01, a Dermorphin–Ranatensin Hybrid Peptide. International Journal of Molecular Sciences, 25(7), 4007.

+ Open protocol

+ Expand

Radioligand Binding Assay for Opioid Receptors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell culture
media and supplements
were obtained from Sigma-Aldrich Chemicals (St. Louis, MO) or Life
Technologies (Carlsbad, CA). Radioligands [³H][d-Ala²,N-Me-Phe⁴,Gly-ol⁵]enkephalin ([³H]DAMGO, 50 Ci/mmol), [³H]diprenorphine (37 Ci/mmol), [³H]U69,593 (39.1 Ci/mmol),
and [³⁵S]GTPγS (1250 Ci/mmol) were purchased from
PerkinElmer (Traiskirchen, Austria). [¹²⁵I]-NT (∼2200
Ci/mmol) was purchased from PerkinElmer (Billerica, MA). DAMGO, [d-Pen²,d-Pen⁵]enkephalin (DPDPE),
U69,593, diprenorphine, tris(hydroxymethyl)aminomethane (Tris), 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic
acid (HEPES), unlabeled GTPγS, and guanosine diphosphate (GDP)
were obtained from Sigma-Aldrich Chemicals (St. Louis, MO). PathHunter
detection reagents were obtained from DiscoverX (Birmingham, UK).
All other chemicals were of analytical grade and obtained from standard
commercial sources. Test peptides were prepared as 1 mM stocks in
water and further diluted to working concentrations in the appropriate
medium.

Gonzalez S., Dumitrascuta M., Eiselt E., Louis S., Kunze L., Blasiol A., Vivancos M., Previti S., Dewolf E., Martin C., Tourwé D., Cavelier F., Gendron L., Sarret P., Spetea M, & Ballet S. (2020). Optimized Opioid-Neurotensin Multitarget Peptides: From Design to Structure–Activity Relationship Studies. Journal of Medicinal Chemistry, 63(21), 12929-12941.

+ Open protocol

+ Expand

Radioligand Binding Assay for Opioid Receptors

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell culture media and supplements were obtained from Sigma-Aldrich Chemicals (St. Louis, MO), or Life Technologies (Carlsbad, CA). Radioligands [³H][D-Ala²,N-Me-Phe⁴,Gly-ol⁵]enkephalin ([³H]DAMGO, 50 Ci/mmol), [³H]Diprenorphine (37 Ci/mmol), and [³⁵S]GTPγS (1250 Ci/mmol) were purchased from PerkinElmer (Boston, MA). [³H]HS665 (30.65 Ci/mmol) was prepared by Dr. Geza Toth (Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary) as previously described^{49 (link)}. DAMGO, [D-Pen²,D-Pen⁵]enkephalin (DPDPE), U69,593, Diprenorphine, Tris(hydroxymethyl) aminomethane (Tris), 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES), Hank’s Balanced Salt Solution (HBSS), unlabeled GTPγS, guanosine diphosphate (GDP) and forskolin were obtained from Sigma-Aldrich Chemicals (St. Louis, MO). Morphine hydrochloride was obtained from Gatt-Koller GmbH (Innsbruck, Austria). Compounds 1a and 2a were synthesized according to the described procedure^{13 (link)}, and 14-OMO (3) and 14-MM (4) were prepared as earlier described^{16 (link),50 (link)}. All other chemicals were of analytical grade and obtained from standard commercial sources.

Dumitrascuta M., Bermudez M., Ben Haddou T., Guerrieri E., Schläfer L., Ritsch A., Hosztafi S., Lantero A., Kreutz C., Massotte D., Schmidhammer H., Wolber G, & Spetea M. (2020). N-Phenethyl Substitution in 14-Methoxy-N-methylmorphinan-6-ones Turns Selective µ Opioid Receptor Ligands into Dual µ/δ Opioid Receptor Agonists. Scientific Reports, 10, 5653.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!