Sodium alginate with a medium viscosity and high mannuronic acid without inulin were purchased from Hi-media (Mumbai). They were prepared in distilled water and autoclaved at 121 °C for 15 min. The low-molecular-weight chitosan (deacetylated chitin, Hi-Media, Laboratories Mumbai), was prepared in distilled water and pure hydrochloric acid (Loba Chemie, Pvt Ltd.—Mumbai, India). Xanthan gum, gum acacia, and carrageenan were purchased from Loba Chemie, Pvt Ltd.—Mumbai, India. Starch, sodium caseinate, pepsin, trypsin, calcium chloride, sodium chloride, sodium bicarbonate, trisodium citrate, sodium hydroxide, potassium chloride, phosphate buffer saline (pH 7.2), and hydrochloric acid were purchased from Hi-media Laboratories (Mumbai, India). Glacial acetic acid with molar mass of 60.05 g mol−1 was bought from Merck (Darmstadt, Germany). Pancreatin, peptone, and bile salt were obtained from Loba Chemie, Pvt Ltd. (Mumbai, India). The De Man Rogosa and Sharpes (MRS) broth and MRS agar used in this work were purchased from Hi-media Laboratories (Mumbai). Indicator microorganisms used for the antimicrobial activity; i.e., Escherichia coli (MTCC No-432), Staphylococcus aureus (MTCC No-96), and Bacillus cereus (MTCC No-430), were procured from the Institute of Microbial Technology (IMTECH; Chandigarh, India).
Potassium chloride (kcl)
Manufactured by HiMedia
Sourced in India
Potassium chloride is a chemical compound that is commonly used as a laboratory reagent. It is a white, crystalline solid that is highly soluble in water. The primary function of potassium chloride is to provide a source of potassium ions for various chemical and biological applications.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Merck Group (5 mentions)
Sodium hydroxide (naoh), by HiMedia (4 mentions)
Sodium chloride (nacl), by HiMedia (4 mentions)
Sodium chloride (nacl), by Merck Group (3 mentions)
Hydrochloric acid (hcl), by HiMedia (2 mentions)
Sodium bicarbonate, by HiMedia (2 mentions)
Ethanol, by Merck Group (2 mentions)
Imidazole, by HiMedia (2 mentions)
Lysozyme, by HiMedia (2 mentions)
Urea, by Avantor (2 mentions)
Ammonium sulfate, by HiMedia (2 mentions)
Q sepharose, by GE Healthcare (2 mentions)
Hepes, by Merck Group (2 mentions)
β mercaptoethanol, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Glycerol, by Merck Group (2 mentions)
Potassium hydroxide, by Merck Group (2 mentions)
Ethylene diamine tetraacetic acid (edta), by HiMedia (2 mentions)
Sodium dodecyl sulfate (sds), by HiMedia (2 mentions)
Ethanol, by HiMedia (2 mentions)
15 protocols using potassium chloride (kcl)
1
Natural Antimicrobial Polymer Formulations
2
Emulsifier-stabilized Sweet Almond Oil Formulation
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Polyethoxylated sorbitan ester Tween 80 (T80), and sorbitan esters Span 80 (S80) and Span 85 (S85), used as emulsifiers, were purchased, respectively, from PanReac AppliChem (Barcelona, Spain), AlfaAesar (Karlsruhe, Germany) and Sigma-Aldrich (Darmstadt, Germany). The sweet almond oil was acquired from LabChem (Santo Antão do Tojal, Portugal), and the cinnamon (Cinnamomum zeylanicum) aqueous extract supplied by Essência D’um Segredo (Seixal, Portugal). The Nile Red was supplied by Sigma-Aldrich (Darmstadt, Germany), and Isopropyl alcohol from Merck (Darmstadt, Germany). Kanamycin sulfate (antibiotic) was purchased from Merck (Darmstadt, Germany), Mueller Hinton II Agar was purchased from Liofilchem (Roseto degli Abruzzi, Italy). For Ringer solution preparation, Sodium Chloride, Sodium Hydrogen Carbonate and Calcium Chloride 2-hydrate were purchased from Pancreac Applichem (Barcelona, Spain), Potassium Chloride was purchased from HiMedia (Mumbai, India). Methanol and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) were purchased from Sigma-Aldrich (Darmstadt, Germany). All reagents were directly used without further purification. Water used in this work was distilled water.
3
Enzymatic Antioxidant Assay Protocol
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2,2 Diphenyl-1-picrylhydrazyl (DPPH), ADH and ALDH were procured from Sigma-Aldrich, Bangalore, India. Bovine serum albumin (BSA), β-mercaptoEthanol, potassium chloride, gallic acid, β-nicotinamide adenine dinucleotide (NAD+) and NADH were purchased from HiMedia Laboratories, Mumbai, India. Ethanol, mEthanol, and acetaldehyde were purchased from Merck Ltd, Mumbai, India.
4
Comprehensive Sourcing of Research Reagents
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All the chemicals, as well as reagents, are of high purity. Dulbecco’s eagle medium (DMEM) and phosphate buffer saline (PBS) were purchased from Sigma, UK. Elisa kits, IL-6, Human TNF-α, were purchased from Invitrogen (USA). From Gibco USA, fetal bovine serum was obtained and lipopolysaccharide (LPS) E. Coli from Calbiochem (USA). Griess reagent was purchased from Promega (USA) and MTT from Calbiochem (San Diego CA). Besides, other chemicals that were used were of research-grade. From the central drug house (CDH) Jammu, ferric chloride, glacial acetic acid, chloroform was purchased. Ascorbic acid, ethanol, methanol, picric acid, calcium chloride, sodium chloride, 2,2 diphenyl picryl hyradyzl (DPPH), sucrose, trichloroacetic acid were procured from Merck. From Qualigens, potassium dihydrogen phosphate, hydrogen peroxide and hydrochloric acid were purchased. Ferric nitrate, ethylene diamine tetraacetate, potassium chloride, sodium hydroxide, dimethyl sulfoxide, sodium dihydrogen monophosphate was purchased from HiMedia. From sisco research laboratories (SRL) butylated hydroxytoluene was bought and from Rankem ethyl acetate and hexane was purchased.
5
Oligomer and Fibril Amyloid Assay
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Sodium phosphate dibasic dihydrate, magnesium chloride hexahydrate, ThT, adenosine-5′-triphosphate disodium salt hydrate (ATP), dimethyl sulfoxide, guanidinium hydrochloride, β-mercaptoethanol, and Hepes were procured from Sigma. Urea and PK were bought from Amresco. SDS, imidazole, ammonium sulfate, lysozyme, potassium chloride, and EDTA were purchased from HIMEDIA. Sodium hydroxide, potassium hydroxide, glycerol, methanol, nitrocellulose membrane, A11 (anti–amyloid oligomer antibody), OC (anti–amyloid fibril antibody), horseradish peroxidase–conjugated goat anti-rabbit antibody, and sodium chloride were bought from Merck. Antibiotics (ampicillin and chloramphenicol) and IPTG were procured from Gold Biocom. Q-sepharose and nickel–nitrilotriacetic acid columns were bought from GE Healthcare Lifesciences. Fluorescein-5-maleimide was procured from Invitrogen. About 96-well NUNC optical bottom plates were purchased from ThermoFisher Scientific.
6
Creatinine Quantification in Aqueous Solutions
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We used argon gas N50, high-purity deionized water, bovine albumin powder (Sigma-Aldrich), creatinine anhydrous (Sigma-Aldrich), potassium chloride (HIMEDIA), sodium chloride (Fluka), sodium phosphate monobasic (Fluka), the SPINREACT Creatinine-J kit, and urea (Sigma-Aldrich).
7
Purification and Characterization of Amyloid Aggregates
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Hepes, magnesium chloride hexahydrate, sodium phosphate dibasic dihydrate, tris (hydroxymethyl) aminomethane (Tris), β-mercaptoethanol, ATP disodium salt hydrate, DTT, and ThT were bought from Sigma. GdmCl, proteinase K, and urea were procured from Amresco. Ammonium sulfate, imidazole, lysozyme, SDS, EDTA, and potassium chloride was bought from HIMEDIA. Potassium hydroxide, sodium chloride, sodium hydroxide, glycerol, A-11 antiamyloid oligomer antibody, methanol, horseradish peroxide (HRP)–conjugated goat anti-rabbit antibody was procured from Merck. IPTG and antibiotics (chloramphenicol and ampicillin) were purchased from Gold Biocom. Enhanced chemiluminescence kit, HRP-conjugated rabbit antimouse antibody, was obtained from Thermo Fisher Scientific. Nickel-nitriloacetic acid (Ni-NTA) column and Q-Sepharose were from GE Healthcare Lifesciences. Phosphoenolpyruvate (PEP) and pyruvate kinase (PK) were procured from Roche Diagnostics.
8
Electrochemical Sensor Fabrication
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Potassium ferricyanide, graphite powder, melamine, and Whatman qualitative filter paper Grade 1 were bought from Sigma-Aldrich. Nucleic acid bases like guanine, adenine, thymine, and sodium hydroxide, potassium chloride, urea, ethanol, acetic acid, hydrochloric acid, potassium dihydrogen phosphate (KH2PO4), N-cetyl-N, N, N trimethyl ammonium bromide (CTAB), and potassium hydrogen phosphate (K2HPO4) were procured from Hi-Media, India.
9
Electrochemical Characterization of CNT-Silane Composites
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The substances 3-aminopropyltrimethoxysilane (APTMS) and tetraethyl orthosilicate (TEOS) and the multiwalled carbon nanotubes (MWCNTs) (95%, L: 20–30 nm, D: 0.5–2 nm, W: 1–2 nm) were purchased from Sigma-Aldrich (Darmstadt, Germany; www.sigmaaldrich.com ). Hypoxanthine, xanthine, caffeine, ferric chloride hexahydrate (FeCl3 × 6H2O), hexadecyl trimethyl ammonium bromide (CTAB), ethylene glycol (EG), sodium chloride, potassium chloride, sodium dihydrogen phosphate, and potassium hydrogen phosphate were purchased from Himedia (Mumbai, Maharashtra, India, http://www.himedialabs.com ). Potassium ferricyanide, potassium ferrocyanide, sodium acetate, ethanol, methanol, ammonium hydroxide solution, acetic acid, hydrochloric acid, sulfuric acid, and nitric acid were obtained from SDFCL (India, http://www.sdfine.com ). All of the reagents were of an analytical grade and were utilized without any further processing. A CH Instruments electrochemical workstation (CHI660D) (CH Instruments, USA) was used for all electrochemical experiments.
10
Comprehensive Reagents for Biochemical Protocols
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Chemicals used in the present study were of highest purity/analytical grade. Sodium chloride, potassium chloride, sodium carbonate, sodium bicarbonate, disodium hydrogen phosphate, sodium dihydrogen phosphate, triton X-100, sodium hydroxide, ethylenediamine tetraacetic acid, reduced Glutathione, sodium azide, ethanol, 3-[N-morpholino] propane sulfonic acid (MOPS buffer), and magnesium chloride were purchased from HIMEDIA Chemicals (Mumbai, India). Glutathione, Folin-Ciocalteu's reagent, glucose-6-phosphate dehydrogenase, glucose-6-phosphate, nicotinamide adenine dinucleotide phosphate (reduced and oxidized), TRI reagent, formamide, trisodium citrate, sodium acetate, and ethidium bromide were purchased from Sigma Aldrich Chemicals (St Louis, MO). One-step reverse transcriptase polymerase chain reaction (RT-PCR) kit and DNA molecular weight markers (100-600 bp) were obtained from Qiagen India Private Limited (New Delhi, India), and PCR primers were obtained from Eurofins Genomics India Private Limited (Bengaluru, India).
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