Charge coupled device camera

Manufactured by Thermo Fisher Scientific

Sourced in United States

A charge-coupled device (CCD) camera is a type of digital camera that uses a CCD image sensor to capture and record visual information. The CCD sensor converts light energy into electrical signals, allowing the camera to generate digital images. The core function of a CCD camera is to provide a reliable and efficient method for capturing and digitizing visual data for various scientific and industrial applications.

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Lab products found in correlation

Charge coupled device camera, by Olympus (1 mentions) Bx53 light microscope, by Olympus (1 mentions) Cellsens, by Olympus (1 mentions) Vitrobot, by Thermo Fisher Scientific (1 mentions) Talos tem, by Thermo Fisher Scientific (1 mentions) Nova nanosem, by Thermo Fisher Scientific (1 mentions) Gatan cryotransfer, by Ametek (1 mentions) Immobilon ny, by Merck Group (1 mentions) Ultrahyb ultrasensitive hybridisation buffer, by Thermo Fisher Scientific (1 mentions) Ultrahyb ultrasensitive hybridization buffer, by Thermo Fisher Scientific (1 mentions) Amersham hybond n, by GE Healthcare (1 mentions)

5 protocols using charge coupled device camera

Ultrastructural Analysis of Mitochondria in Ischemic Myocardium

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Ventricular sections isolated from the anterior left ventricular myocardium were obtained from hearts 2 hours post cardiac arrest resuscitation. They were fixed overnight in in 2.5% glutaraldehyde / 4% paraformaldehyde in 0.1 M sodium cacodylate buffer and maleate buffer (pH 5.1) and processed as previously described (n=3 hearts/group) (29 (link)). Images were collected using a scanning transmission electron microscope (TEM) at 300 KV (Tecnai F30; FEI) with a Gatan charge-coupled device camera. Blinded observers using Image J software analyzed Mitochondrial images. A minimum of 600 mitochondria per group underwent morphometric analysis.

Sharp W.W., Beiser D.G., Fang Y.H., Han M., Piao L., Varughese J, & Archer S.L. (2015). Inhibition of the Mitochondrial Fission Protein Drp1 Improves Survival in a Murine Cardiac Arrest Model. Critical care medicine, 43(2), e38-e47.

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Bright-field and Electron Microscopy Imaging

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Bright-field images of histology were taken on a BX53 light microscope (Olympus, Tokyo, Japan) using a charge-coupled device camera (Olympus). To obtain representative images across several fields, a digital stitching tool (Cell Sens, Olympus) was used and images were assembled using Photoshop (Adobe Systems Inc., San Jose, CA). Subcellular images were taken using a Tecnai Biotwin 12 transmission electron microscope (FEI, Hillsboro, OR) equipped with a charge-coupled device camera (FEI). Tissue from a minimum of three individuals was observed to make conclusions. All representative images in figures represent one of at least n=3 biological replicates that were observed, unless otherwise noted in figure legends.

Gawriluk T.R., Simkin J., Thompson K.L., Biswas S.K., Clare-Salzler Z., Kimani J.M., Kiama S.G., Smith J.J., Ezenwa V.O, & Seifert A.W. (2016). Comparative analysis of ear-hole closure identifies epimorphic regeneration as a discrete trait in mammals. Nature Communications, 7, 11164.

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Cryo-TEM and SEM Analysis of Liposomal Formulations

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Morphology of liposomal suspensions before USFD were evaluated by cryo-TEM (Angelov et al., 2015 (link)). Liposomal suspensions of 3 μL were placed on a TEM copper grid covered with a perforated carbon film and then blotted with filter paper to obtain a thin aqueous film. The grid was vitrified immediately by plunging the grid (kept at 100 % humidity and 22°C) into liquid ethane maintained at its melting point using a Vitrobot (FEI Company, Hillsboro, OR, USA). The vitreous films were transferred to a Talos TEM (FEI Company, Hillsboro, OR, USA) using a Gatan cryotransfer (Gatan, Pleasanton, CA, USA), and the samples were observed in a low-dose mode. Images were acquired at 300 kV at a temperature around −173°C with a charge-coupled device camera (FEI Company, Hillsboro, OR, USA).
The morphology of Cipro-Col-Lips dry powder prepared using the USFD method was obtained by the scanning electron microscope (SEM, NOVA nanoSEM, FEI Company, Hillsboro, OR, USA) at 5.0 kV. Each formulation powder was poured on a carbon sticky tape attached to the metal stubs. The stubs were then placed in a sputter coater (208 HR, Cressington Sputter Coater, England, UK) to obtain gold coating at 40 mA for 90 s to achieve a coating thickness of 10 – 40 nm.

Yu S., Wang S., Zou P., Chai G., Lin Y.W., Velkov T., Li J., Pan W, & Zhou Q.T. (2019). Inhalable Liposomal Powder formulations for Co-delivery of Synergistic Ciprofloxacin and Colistin against Multi-drug Resistant Gram-negative Lung Infections. International journal of pharmaceutics, 575, 118915.

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Northern Blot Analysis of comEA and mreB

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Northern-blot analysis were performed as previously described39 (link). Briefly, three micrograms of total RNA in denaturing buffer were loaded per lane and run on denaturing formaldehyde 1.5% agarose gel in MOPS buffer. RNA were transferred to nylon membrane (IMMOBILON-NY+, Millipore corporation) by capillary transfer in 10X SSC buffer (Saline Sodium Citrate 1X: 150 mM NaCl + 15 mM sodium citrate) and then cross-linked to the membrane by UV irradiation. Membrane were hybridised at 42 °C with 5′-biotinylated oligonucleotide probes (5 nM) for either comEA (comEA2-NB) or mreB (mreB-NB) in ULTRAhyb Ultrasensitive Hybridisation Buffer (Ambion, Austin, TX). Following overnight hybridisation the membranes were washed twice in 2X SSC buffer containing 0.1% SDS at 65 °C according to the ULTRAhyb manufacturer instructions. Probed membranes were revealed using horseradish peroxidase-conjugated streptavidin and enhanced luminol substrate (Chemiluminescent Nucleic Acid Detection Module, Pierce, Rockford, IL). Luminescence signals were acquired using an imaging workstation equipped with a charge-coupled device camera (Thermo).

Juan P.A., Attaiech L, & Charpentier X. (2015). Natural transformation occurs independently of the essential actin-like MreB cytoskeleton in Legionella pneumophila. Scientific Reports, 5, 16033.

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Quantitative Northern Blot Analysis

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Northern-blot analysis was performed as previously described42 (link). One microgram of total RNA in TBE (Tris, Borate, EDTA)-Urea denaturing buffer (Thermo Fischer) was loaded per lane and run on denaturing urea 6% acrylamide gel in TBE buffer. Total RNAs were visualized by ethidium bromide staining to check for equal loading of the lanes. RNAs were transferred to a charged nylon membrane (Amersham Hybond-N+, GE Healthcare Science) by electrophoretic transfer (30 min, 300 mA) and then cross-linked to the membrane by UV irradiation. Membrane were hybridised at 42 °C with 5′-biotinylated oligonucleotide probes (5 nM) for tmRNA in ULTRAhyb Ultrasensitive Hybridization Buffer (Ambion). Following overnight hybridisation, the membranes were washed twice in 2X SSC buffer containing 0.1% SDS at 65 °C according to the ULTRAhyb manufacturer instructions. Probed membranes were revealed using horseradish peroxidase-conjugated streptavidin and enhanced luminol substrate (Chemiluminescent Nucleic Acid Detection Module, Pierce). Luminescence signals were acquired using an imaging workstation equipped with a charge-coupled device camera (Thermo Scientific).

Brunel R, & Charpentier X. (2016). Trans-translation is essential in the human pathogen Legionella pneumophila. Scientific Reports, 6, 37935.

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