Paraffin or frozen 5 µm sections of skin tissue samples were stained with the following antibodies: CD31 antibody (1:100, ab28364 Abcam) for detection of endothelial cells; Calponin (1:100, EP798Y, Abcam) and α-SMA (1:200, ab5694, Abcam) for detection of perivascular cells, and F4/80 (1:100, ab6640, Abcam) for assessing macrophages in vivo. Collagen type I (1:200, ab21286, Abcam), collagen type IV (1:100, AB756, Chemicon), collagen type XVIII (1:100, sc-32720, Santa Cruz), collagen type VI (1:50, sc-167530, Santa Cruz), and fibronectin (1:500, sc-9068, Santa Cruz) were used for extracellular matrix and basement membrane evaluation. Incubation with primary antibody was followed by FITC or Texas Red labeled secondary antibody (1:500, Vector Laboratories). Nuclei were stained with Hoechst 33342 fluorescent dye (Thermo Scientific).
Fitc or texas red labeled secondary antibody
Manufactured by Vector Laboratories
FITC or Texas Red labeled secondary antibody is a laboratory reagent used for the detection and visualization of target proteins in various experimental techniques, such as immunofluorescence and Western blotting. These secondary antibodies are conjugated with fluorescent dyes, either FITC (Fluorescein Isothiocyanate) or Texas Red, which emit light at specific wavelengths when excited, allowing for the specific labeling and detection of the target protein.
Automatically generated - may contain errors
Lab products found in correlation
Hoechst 33342 fluorescent dye, by Thermo Fisher Scientific (2 mentions)
Ep798y, by Abcam (2 mentions)
Ab756, by Merck Group (2 mentions)
Sc 32720, by Santa Cruz Biotechnology (2 mentions)
Sc 167530, by Santa Cruz Biotechnology (2 mentions)
Sc 9068, by Santa Cruz Biotechnology (2 mentions)
Ab28364, by Abcam (2 mentions)
Ab6640, by Abcam (2 mentions)
Ab21286, by Abcam (2 mentions)
Ab5694, by Abcam (2 mentions)
2 protocols using fitc or texas red labeled secondary antibody
1
Comprehensive Immunohistochemistry Protocol for Skin Tissue
2
Comprehensive Immunohistochemistry Protocol for Skin Tissue
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Paraffin or frozen 5 µm sections of skin tissue samples were stained with the following antibodies: CD31 antibody (1:100, ab28364 Abcam) for detection of endothelial cells; Calponin (1:100, EP798Y, Abcam) and α-SMA (1:200, ab5694, Abcam) for detection of perivascular cells, and F4/80 (1:100, ab6640, Abcam) for assessing macrophages in vivo. Collagen type I (1:200, ab21286, Abcam), collagen type IV (1:100, AB756, Chemicon), collagen type XVIII (1:100, sc-32720, Santa Cruz), collagen type VI (1:50, sc-167530, Santa Cruz), and fibronectin (1:500, sc-9068, Santa Cruz) were used for extracellular matrix and basement membrane evaluation. Incubation with primary antibody was followed by FITC or Texas Red labeled secondary antibody (1:500, Vector Laboratories). Nuclei were stained with Hoechst 33342 fluorescent dye (Thermo Scientific).
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