Bicuculline methochloride

Manufactured by Abcam

Sourced in United Kingdom

Bicuculline methochloride is a chemical compound commonly used as a laboratory tool in neuroscience research. It functions as a competitive antagonist of the GABA-A receptor, blocking the action of the neurotransmitter gamma-aminobutyric acid (GABA).

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Lab products found in correlation

Bumetanide, by Bio-Techne (1 mentions) Furosemide, by Bio-Techne (1 mentions) Fluozin 3 am, by Thermo Fisher Scientific (1 mentions) Platelet derived growth factor, by Merck Group (1 mentions) Rabbit anti kcc2, by Merck Group (1 mentions) Rabbit anti actin, by Merck Group (1 mentions) Sulfasalazine, by Merck Group (1 mentions) Gramicidin, by Merck Group (1 mentions) D apv, by Abcam (1 mentions) Tetrodotoxin citrate, by Abcam (1 mentions) Periflux system 5000, by Perimed (1 mentions) Etomidate, by Bio-Techne (1 mentions) Anhydrous grade solvents, by Merck Group (1 mentions)

3 protocols using bicuculline methochloride

Pharmacological Modulation of Neuronal Signaling

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GABA, Bicuculline methochloride, Tetrodotoxin citrate, NBQX and D-APV were purchased from Abcam Biochemicals (Cambridge, UK); Furosemide (100 mM in dimethyl sulfoxide (DMSO)), Bumetanide (50 mM in EtOH) and PP2 (10 mM in DMSO) were purchased from Tocris Biosciences (Bristol, UK); FluoZin-3AM (Invitrogen, 5 mM DMSO); K252a (Calbiochem, Merck S.p.a., Milano, Italy); DIOA (Santa Cruz Biotechnology, Inc., Heidelberg, Germany; 200 mM in EtOH); BDNF (PeproTech EC Ltd., London, UK); TPEN (10 mM in EtOH), Gramicidin (50 mg/ml in DMSO), Sulfasalazine (500 mM in DMSO), platelet-derived growth factor and all chemicals used were purchased from Sigma-Aldrich, Milan, Italy. Where not indicated, all drugs were dissolved in water. Antibodies were purchased and used as follows: rabbit anti-Phospho TrkB (phospho Y515, 1 : 500, Abcam, Cambridge, UK), rabbit anti-Phospho Src (phospho Y416, 1 : 2000, Abcam), mouse anti- Neuronal Class III β-tubulin (TuJ1, 1 : 2000, n.cat MMS-435P, Covance, San Diego, CA, USA), rabbit anti-actin (1 : 5000, Sigma-Aldrich); rabbit anti-KCC2 (1 : 1000; Millipore, Temecula, CA, USA).

Di Angelantonio S., Murana E., Cocco S., Scala F., Bertollini C., Molinari M.G., Lauro C., Bregestovski P., Limatola C, & Ragozzino D. (2014). A role for intracellular zinc in glioma alteration of neuronal chloride equilibrium. Cell Death & Disease, 5(10), e1501-.

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Epileptic Discharges and Cortical Blood Flow

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The animal was equipped with one tungsten electrode and one Doppler electrode, located above the somatosensory cortex. LFP were recorded by sharpened tungsten electrodes lowered to 500 μm into the cortex, close to the bicuculline injection site. CBF was recorded by a laser Doppler system (Perimed Periflux System 5000, Stockholm, Sweden; 0.03 s time constant, 780 nm laser). To measure CBF as locally as possible, we used a needle probe (Perimed probe 411) with a small separation (0.15 mm) between emitting and collecting light fibers [2 (link)]. To elicit epileptic discharges, bicuculline methochloride (2.5 mM, Abcam, UK) was infused at a rate of 200 nl/min during 5 min (1 μl total infusion) using a 5 μl microsyringe (Hamilton, 75RN neuro syringe) mounted to a micropump at a depth between 1000 and 1500 μm targeting the cortical layers III to VI. Epileptic discharges appeared about 7 s after the onset of the infusion.

Blanchard S., Saillet S., Ivanov A., Benquet P., Bénar C.G., Pélégrini-Issac M., Benali H, & Wendling F. (2016). A New Computational Model for Neuro-Glio-Vascular Coupling: Astrocyte Activation Can Explain Cerebral Blood Flow Nonlinear Response to Interictal Events. PLoS ONE, 11(2), e0147292.

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Purification and Storage of GABAA Receptors

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11α-Hydroxy-progesterone 16 was from Santa Cruz Biotechnology, Inc. Allopregnanolone [5α-pregnan-3α-ol-20-one (3α,5α-P)], alphaxalone (5α-pregnan-3α-ol-11,20-dione), and etomidate were from Tocris Bioscience, and 5α-pregnan-3α, 21-diol-20-one (3α,5α-THDOC) was from Steraloids, Inc. Pregnanolone [5β-pregnan-3α-ol-20-one (3α,5β-P)] was from Research Plus and pregnenolone sulfate (PS) was from Santa Cruz Biotechnology. Bicuculline methochloride was from Abcam. Human α1β3 and 3α,21-Dihydroxy-5α-pregnan-20-one 3, TFD-benzoic acids 21 and 22 were prepared according to the known methods.^{25 ,26 (link)} Anhydrous grade solvents were from Aldrich, and were not further dried or purified. R-mTFD-MPAB was synthesized as described previously.^{29 (link)} α1β3γ2 GABA_ARs with a FLAG epitope on the N-terminus of α1 subunit were expressed in tetracycline-inducible HEK293S cells and purified from detergent extracts as described^{18 (link),19 (link),33 (link),34 (link)} by use of an anti-FLAG antibody column. After elution from columns in the presence of 0.1 mM FLAG peptide, purified GABA_ARs were stored at −80^○C until use in elution buffer containing 5 mM CHAPS and 0.2 mM asolectin.

Wu B., Jayakar S.S., Zhou X., Titterton K., Chiara D.C., Szabo A.L., Savechenkov P.Y., Kent D.E., Cohen J.B., Forman S.A., Miller K.W, & Bruzik K.S. (2018). Inhibitable Photolabeling by Neurosteroid Diazirine Analog in the β3-Subunit of Human Hetereopentameric Type A GABA Receptors. European journal of medicinal chemistry, 162, 810-824.

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