Anti usp33

The following reagents were used: anti-HERC2 monoclonal (BD Biosciences #612366); anti-HERC2 polyclonal (Bvg1 antibodies against residues 4785-4834, and Bvg9 antibodies against residues 1-199) [15 (link)]; anti-p21 (C-19), anti-p53 (FL-393), anti-NEURL4 (E-20) (Santa Cruz Biotechnology, Inc.); anti-p53 Ab-5 (DO-7) (Neo Markers); anti-GST monoclonal (GenScript); anti-Ran [34 (link)]; anti-α-tubulin (Ab-1) (Calbiochem); anti-USP33 (Proteintech); anti-Flag M2 (Sigma); anti-GFP and anti-c-myc (clone 9E10) (Roche); anti-MDM2 (2A10) (Abcam); Z-Leu-Leu-Leu-al (MG132) (Sigma-Aldrich); horseradish peroxidase-conjugated secondary antibodies; lipofectamine LTX (Invitrogen); cycloheximide (Applichem); protein A-Sepharose and glutathione-Sepharose (GE Healthcare); GFP-Trap_A (ChromoTek); Immobilon-P PVDF transfer membrane (Millipore Corporation); luciferase assay system (Promega); luminescent β-galactosidase detection Kit II (Clontech Laboratories).

The following antibodies were used in this study: anti-USP33, anti-slug, and anti-twist1 antibodies were purchased from ProteinTech Group (Chicago, IL, USA); anti-CXCR4 antibody was purchased from BD Bioscience (Franklin Lakes, NJ, USA); anti-HA and anti-Flag M1 antibodies were from Sigma-Aldrich (St. Louis, MO, United States); anti-ppERK, anti-ERK, and anti-β-arrestin2 antibodies were from Cell Signaling Technology (Boston, MA, USA); anti-E-cadherin, anti-N-cadherin, anti-snai1, and anti-β-actin antibodies were purchased from Santa Cruz (Dallas, TX, USA).
N-ethylmaleimide (NEM, deubiquitinase inhibitor), SDF-1 (SDF-1α, CXCR4 agonist), AMD3100 octahydrochloride hydrate (1,1′-[1,4-Phenylenebis(methylene)]bis-1,4,8,11-tetraazacyclotetradecane octahydrochloride, CXCR4 antagonist), dynasore hydrate (3-Hydroxy-naphthalene-2-carboxylic acid (3,4-dihydroxy-benzylidene)-hydrazide hydrate, dynamin inhibitor), HA affinity beads, and Flag affinity beads were all purchased from Sigma-Aldrich.

The following antibodies were used: anti-HERC2 monoclonal (BD Biosciences); anti-HERC2 polyclonal [32 (link)]; anti-p21 (C-19); anti-p62 (SQSTM1 (D-3): sc-28359); anti β-actin (Santa Cruz Biotechnology, Inc.); anti-calbindin D-28k polyclonal (Cb-38a, Swant); anti-calbindin D-28k monoclonal (Cb-955, Sigma); anti-p53 Ab-5 (DO-7) (Neo Markers); anti-USP33 (Proteintech); anti-Ran [62 (link)]; anti α-tubulin (Ab-1, Calbiochem); Alexa Fluor^® 488 donkey-anti-rabbit (A21207), and Alexa Fluor^® 594 donkey-anti-mouse (A21203) (Invitrogen); horseradish peroxidase-conjugated secondary antibodies (Invitrogen); biotin-conjugated secondary antibodies (Vector); and the Avidine-Streptavidine Elite Kit (Vector).