DCs were treated with immethridine (1 μM) and LPS (100 ng/ml) for 24h. Naïve CD4+ T cells were prepared by Mouse CD4 Negative Selection Kit (Stemcell Technology, Canada) from spleens of C57BL/6 mice and co-cultured with immethridine-treated or untreated DCs at a ratio of 10:1 for 72h at the present of 0.5 μg/ml anti-CD3 mAb and 5 μg/ml anti-CD28 mAb (BD Biosciences). The percentage of Th1 and Th17 cells was analyzed by intracellular staining with FITC-labeled anti-IFN-γ or FITC-labeled anti-IL-17A (BD Biosciences, San Jose, CA).
Mouse cd4 negative selection kit
Manufactured by STEMCELL
Sourced in Canada
The Mouse CD4 Negative Selection Kit is a laboratory tool used to isolate CD4-negative cells from mouse samples. It utilizes a magnetic separation technique to negatively select for non-CD4 cells, allowing for the isolation of desired cell populations.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mouse cd4 negative selection kit
1
Immethridine-treated DCs Modulate T Cell Differentiation
2
MOG-Specific T Cell Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DCs were incubated with immethridine and MOG35-55 (20 μg/ml) for 4h. After washed with PBS, DCs were stimulated with LPS (100 ng/ml) for 12h. 1×106 of CD4+ T cells, isolated from splenocytes of EAE mice with Mouse CD4 Negative Selection Kit (Stemcell Technology, Canada), were co-cultured with 1×105 of MOG35-55 peptide-pulsed DCs at a ratio of 10:1 ratio in 96-well round bottom plates for 72h. Finally, cells were analyzed by MTT Cell Proliferation Assay Kit according to the manufacturer’s instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!