The NMR spectra (1H and 13C) were recorded on four different Bruker instruments including 400 MHz, 500 MHz, 600 MHz, 800 MHz. Chemical shifts are given in δ (ppm) value relative to TMS as internal standard. Deuterated solvents were used to dissolve the samples for NMR experiments. The HR-ESI-MS spectra were obtained from Bruker Compact QToF and MAXIS II mass spectrometers. EI-MS and FAB-MS data were recorded on a Jeol JMS HX 110 mass spectrometer. Silica gel (230–400 meshes) was used for column chromatography. Thin Layer Chromatography (TLC) and preparative TLC were performed on precoated silica gel plates (60 F254, Macherey-Nagel) using various solvent systems as eluent. Spots were visualized using UV light (λmax 254 and 366 nm) and diluted sulphuric acid (10%).
Maxis 2
Manufactured by Bruker
Sourced in United States, Germany
The MaXis II is a high-performance time-of-flight mass spectrometer (TOF-MS) designed for accurate mass measurements and high-resolution analysis. It delivers precise mass determination, high-speed data acquisition, and robust performance for a wide range of analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Compact qtof, by Bruker (1 mentions)
Jms hx 110, by JEOL (1 mentions)
Ascentis express c18 hplc column, by Merck Group (1 mentions)
Prominence uflc xr system, by Shimadzu (1 mentions)
Sep pak, by Waters Corporation (1 mentions)
Potassium hydroxide, by Merck Group (1 mentions)
Luna c18 2, by Phenomenex (1 mentions)
Waters 1515, by Waters Corporation (1 mentions)
Maxis 2 ultra high resolution q tof mass spectrometer, by Bruker (1 mentions)
1290 uhplc system, by Agilent Technologies (1 mentions)
1290 infinity, by Phenomenex (1 mentions)
Smartformula, by Bruker (1 mentions)
1100 hplc, by Agilent Technologies (1 mentions)
Dionex ultimate 3000 uplc, by Thermo Fisher Scientific (1 mentions)
Acquity uplc beh c18 1.7 m column, by Waters Corporation (1 mentions)
Amazon speed, by Bruker (1 mentions)
Bovine serum albumin (bsa), by Bruker (1 mentions)
Acquity beh c18, by Waters Corporation (1 mentions)
Dionex ultimate 3000 rs, by Thermo Fisher Scientific (1 mentions)
16 protocols using maxis 2
1
Spectroscopic Characterization of Compounds
2
Spectroscopic Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
NMR spectra were acquired with a 300 MHz Bruker Avance spectrometer (300.13 MHz for 1H and 75.5 MHz for 13C) in CDCl3 or DMSO-d6 and were referenced to residual solvent proton signals (δH = 7.26 and 2.50, respectively) and solvent carbon signals (δC = 77.16 and 39.52, respectively). Multiplicities are abbreviated as follows: s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet, br = broad, dd = doublet of doublets, dt = doublet of triplets, ddd = doublet/doublets of doublets; coupling constants, J, are reported in Hz. Mass spectra were acquired with an HRMS-ESI-qTOF spectrometer Nexera LCMS9030 or MaXis II Bruker Daltonic GmbH (electrospray ionization mode, positive ions detection). Flash column chromatography on silica (Merck, 230–400 mesh) was performed with a Biotage Isolera Prime instrument. TLC was performed on aluminum-backed pre-coated plates (0.25 mm) with silica gel 60 F254 with a suitable solvent system and was visualized using UV fluorescence.
3
Quantifying Collagen Post-Translational Modifications
4
Mass Spectrometry Analysis of AvrRxo1 Products
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Molecular masses of AvrRxo1products purified from in vitro reactions and small metabolite extracts were determined on a Bruker maXis II mass spectrometer (Bruker Corp., Billerica, MA) following dilution in deionized water. Compounds were fragmented by collision-induced dissociation at −45 eV for phosphorylated NAD and −40 eV for phosphorylated NAAD.
5
Synthesis and Characterization of Radiopharmaceutical Precursor
6
Mass Spectrometry Analysis of gRNA Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All gRNA samples were prepared at 10 mg/mL in
water and injected
(20 μL) on a Thermo Accucore, C30, 2.6 μM, 2.1 mm ×
250 mm column (Waltham, MA) using a Waters H-Class Quaternary UPLC
(Milford, MA) directly coupled to a Bruker Daltonics maXis II ultrahigh
resolution QTOF mass spectrometer (Billerica, MA). Mobile phase A
(MPA) was 100 mM of 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), 16.3
mM of triethylamine (TEA), and 1% methanol; mobile phase B (MPB) was
50% methanol and 50% acetonitrile; and mobile phase C (MPC) was 50%
methanol and 0.1% formic acid. MPC was used as a wash solution at
the end of the gradient to reduce metal adducts in the LC-MS system.50 (link) The column was run at an ambient temperature
with a flow rate of 250 μL/min and a linear gradient of 95:5%
MPA/MPB to 50:50% MPA/MPB over 5 min followed by 3 min of MPC and
4 min of equilibration. The Bruker maXis II was set to negative polarity,
a spectral rate of 1.00 Hz, a source capillary of 4500 V, a source
dry gas of 8.0 L/min, a source dry temperature of 220 °C, an
MRM mass of 1439.5 m/z, an MRM width
of 200.0 m/z, and an MRM collision
energy of 34.0 eV.
water and injected
(20 μL) on a Thermo Accucore, C30, 2.6 μM, 2.1 mm ×
250 mm column (Waltham, MA) using a Waters H-Class Quaternary UPLC
(Milford, MA) directly coupled to a Bruker Daltonics maXis II ultrahigh
resolution QTOF mass spectrometer (Billerica, MA). Mobile phase A
(MPA) was 100 mM of 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), 16.3
mM of triethylamine (TEA), and 1% methanol; mobile phase B (MPB) was
50% methanol and 50% acetonitrile; and mobile phase C (MPC) was 50%
methanol and 0.1% formic acid. MPC was used as a wash solution at
the end of the gradient to reduce metal adducts in the LC-MS system.50 (link) The column was run at an ambient temperature
with a flow rate of 250 μL/min and a linear gradient of 95:5%
MPA/MPB to 50:50% MPA/MPB over 5 min followed by 3 min of MPC and
4 min of equilibration. The Bruker maXis II was set to negative polarity,
a spectral rate of 1.00 Hz, a source capillary of 4500 V, a source
dry gas of 8.0 L/min, a source dry temperature of 220 °C, an
MRM mass of 1439.5 m/z, an MRM width
of 200.0 m/z, and an MRM collision
energy of 34.0 eV.
7
UHPLC-ESI-QTOF-UHRMS Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All mass spectrometry experiments were performed on a 1290 UHPLC system (Agilent, Santa Clara, CA, USA) equipped with DAD, ELSD and maXis II™ (Bruker, Billerica, MA, USA) ESI‐QTOF‐UHRMS with the gradient: 0 min: 95% A; 0.30 min: 95% A; 18.00 min: 4.75% A; 18.10 min: 0% A; 22.50 min: 0% A; 22.60 min: 95% A; 25.00 min: 95% A (A: H2O, 0.1% formic acid (FA); B: acetonitrile, 0.1% FA; flow: 600 μl min−1). Column oven temperature: 45°C. Column: Acquity UPLC BEH C18 1.7 μm (2.1 × 100 mm) with Acquity UPLC BEH C18 1.7 μm VanGuard Pre‐Column (2.1 × 5 mm). Injection volume was either 1 or 2 µl.
8
Characterizing Marine Cyanobacterial Metabolites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Selected VLC-derived marine cyanobacterial fractions were analyzed in the positive-ion-mode electrospray ionization using an MS system (Bruker MAXIS II equipped with a Quadrupole-Time-of-Flight mass analyzer) coupled to an HPLC (Agilent 1290 Infinity equipped with a diode array detector) on a Phenomenex analytical C18 column (2.5 µm, 100 Å, 4.6 × 150 mm). Each fraction was eluted with a starting mobile phase of 5% ACN:95% H2O (0.1% formic acid), followed by a gradient of up to 100% ACN for 15 min at a flow rate of 1 mL/min. The raw data file was analyzed using compact Data Analyst (Bruker software) to provide accurate and high-resolution mass per charge molecular ions in the fractions and generate molecular formulae using a Bruker SmartFormula manually.
9
Covalent Modification of Src Kinase Domain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HPLC was done by using C18 reverse-phase HPLC column. Control sample was prepared by incubating 0.5 mg/ml Src-KD with 5 M Guanidinium chloride (Gu.HCl) for 10 min. To determine if 4 is covalently modifying the Src kinase domain, 0.5 mg/ml Src was incubated with an equimolar ratio of 4 for 1 h and then the sample was treated with 5 M Gu.HCl for 10 min. In total, 100 μl of each sample was injected into the column that was pre-equilibrated with 5% CH3CN + 95% water +0.1% TFA. Protein was eluted by a linear gradient of 10 to 90% CH3CN+0.1% TFA. The elution of protein and 4 was monitored by measuring the absorbance at 280 and 416 nm, respectively. The pure eluted fractions were used for ESI-MS mass spectrometry (Bruker maXis II instrument).
10
Synthesis and Analysis of Bismuth-based Materials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MS samples were prepared by mixing CEF and bismuth nitrate in water, following by addition of NH4OH to adjust the pH to ~7. The mixture was incubated at room temperature for 1 h with gentle shaking. Then the mixed solution was diluted by methanol to a final concentration of 1 μM for the MS examination. Sodium formate was used as an internal standard. Mass Spectrometry was performed and analyzed by UHR TOF LC-MS system, Bruker Maxis II and Bruker data analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!