Anti mouse nlrp3

Nigericin, dimethyl sulfoxide, and ultrapure lipopolysaccharide (LPS) were purchased from Sigma (Munich, Germany). SolB, WeD, and colchicine were obtained from TargetMol (Boston, MA, United States). Anti-mouse-Collagen1 (1:1000), anti-mouse-Smad2/3 (1:1000), and anti-mouse-P-Smad3 (1:1000) were purchased from Cell Signaling Technology (Boston, MA, United States). Anti-mouse-P-IκBα (1:1000), anti-mouse-IκBα (1:1000), anti-mouse -P-IKK (1:1000), anti-mouse-IKK (1:1000), anti-human Smad3 (1:1000), anti-human P-Smad3 (1:1000), and anti-human α-SMA (1:1000) were purchased from Proteintech (Chicago, IL, United States). Anti-mouse-IL-1β, anti-mouse-NLRP3 (1:2000), and anti-mouse-ASC (1:1000) were purchased from Santa Cruz Biotechnology (Beijing, China). Anti-mouse -caspase-1 p20 (1:1000), anti-mouse-caspase-1 p45 (1:1000), anti-mouse-pro-IL-1β (1:1000), and anti-GAPDH (1:2000) were purchased from Proteintech (Chicago, IL, United States).

Dimethyl sulfoxide and ultrapure lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (Munich, Germany). Apigenin, esculetin, gomisin N, schisanhenol, schisandrin A, schisandrin B, anwulignan, schisantherin A, schisantherin B, specnuezhenide, schisandrin, luteolin, quinic acid, curcumenol, and acetaminophen (APAP) were obtained from MCE (New Jersey, NJ, United States). Anti-mouse-IL-1β, anti-mouse-NLRP3, and anti-mouse-ASC antibodies were purchased from Santa Cruz Biotechnology (Beijing, China). Anti-mouse-caspase-1 p45, anti-mouse-pro-IL-1β, and anti-GAPDH antibodies were purchased from Proteintech (Chicago, IL, United States). MitoSOX was purchased from Invitrogen (Carlsbad, CA, United States).