Anti caspase 1 sc 56036

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-caspase-1 (sc-56036) is a primary antibody produced by Santa Cruz Biotechnology. It is designed to detect caspase-1 expression.

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3 protocols using anti caspase 1 sc 56036

Western Blot Analysis of Cell Signaling

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Tissue lysates and 3T3-L1 cells were homogenized with RIPA buffer (Thermo Scientific, Rockford, IL, United States). Protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Western blotting was performed as described previously [15 (link)]. Anti-GSDMD (ab219800) antibody was purchased from Abcam (Cambridge, UK), and anti-adiponectin (AG-20A-0003) antibody was procured from AdipoGen (Seoul, Korea). Anti-Bax (#14796), anti-GPX4 (#59735), and anti-β-tubulin (#2146) antibodies were purchased from Cell Signaling Technology (Danvers, MA), and anti-caspase-1 (sc-56036) and anti-β-actin (sc-47778) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).

Ma E.B., Javaid H.M., Jung D.H., Park J.H, & Huh J.Y. (2022). Gasdermin D Deficiency Does Not Protect Mice from High-Fat Diet-Induced Glucose Intolerance and Adipose Tissue Inflammation. Mediators of Inflammation, 2022, 7853482.

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Immunohistochemical Analysis of Oxidative Stress and Inflammatory Markers

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Pancreas and lung sections were incubated with the following primary antibodies: Anti-NRF2 (sc-365949, 1:200, Santa Cruz Biotechnology, CA, USA); anti-HO-1 (sc-136960, 1:200, Santa Cruz Biotechnology, CA, USA); anti-Mn-SOD (sc-137254, 1:200, Santa Cruz Biotechnology, CA, USA); anti-NLRP3 (sc-134306, 1:200, Santa Cruz Biotechnology, CA, USA); anti-Caspase-1 (sc-56036, 1:200, Santa Cruz Biotechnology, CA, USA); and anti-ASC (sc-514414, 1:200, Santa Cruz Biotechnology, CA, USA), as previously described [48 (link)]. Sections were then incubated with the following secondary antibodies: Peroxidase-conjugated bovine anti-mouse immunoglobulin G (IgG) or peroxidase-conjugated goat anti-rabbit IgG (1:2000, Jackson Immuno Research, West Grove, PA, USA). Specific marking was revealed with a biotin-conjugated goat anti-rabbit IgG or biotin-conjugated goat anti-mouse IgG and avidin-biotin peroxidase complex (Vector Laboratories, Burlingame, CA, USA). Graphic presentation of densitometric analyses was performed Image J software (v1.52a) as previously described [49 (link)]. All immunohistochemical analyses were conducted by an observer without knowledge of the treatments.

Cordaro M., Fusco R., D’Amico R., Siracusa R., Peritore A.F., Gugliandolo E., Genovese T., Crupi R., Mandalari G., Cuzzocrea S., Di Paola R, & Impellizzeri D. (2020). Cashew (Anacardium occidentale L.) Nuts Modulate the Nrf2 and NLRP3 Pathways in Pancreas and Lung after Induction of Acute Pancreatitis by Cerulein. Antioxidants, 9(10), 992.

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Western Blot Analysis of Protein Expression

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Western blot analysis of protein expression was performed as in our previously described protocol (36 (link)). Following quantification of protein lysates from BMMSCs, the samples (50 µg) were loaded on an SDS-PAGE gel and electroblotted onto polyvinylidene difluoride (PVDF) membranes with use of an electrophoretic transfer apparatus (Bio-Rad Laboratories) overnight. PVDF membranes were blocked with 5% fat-free dry milk solution and incubated with primary antibodies (1:1,000 dilution, anti-FNDC5/Irisin antibody, no. ab131390; Abcam), anti-NLRP3 antibody (ab263899; Abcam); anti–caspase-1 (sc-56036; Santa Cruz Biotechnology); anti–cleaved GSDMD (50928; Cell Signaling Technology); anti–caspase-3 (sc-56053; Santa Cruz Biotechnology); anti-RUNX2 (sc-390351; Santa Cruz Biotechnology); anti-OCN (ab93876; Abcam), and anti-GAPDH 6C5 (CB1001; Merck Millipore) for 2 h at room temperature. The PVDF membranes were then incubated with secondary antibody conjugated with horseradish peroxidase (1:5,000) at room temperature for 1 h, followed by chemiluminescence detection (Clarity Max Western ECL Substrate; Bio-Rad Laboratories). The protein bands were quantitatively analyzed with an Image Lab Software system (Bio-Rad Laboratories).

Behera J., Ison J., Voor M.J, & Tyagi N. (2022). Exercise-Linked Skeletal Irisin Ameliorates Diabetes-Associated Osteoporosis by Inhibiting the Oxidative Damage–Dependent miR-150-FNDC5/Pyroptosis Axis. Diabetes, 71(12), 2777-2792.

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