The luc-SKOV-3 ovarian cancer cell line was provided by Dr. Michael Birrer (University of Arkansas), and the OVCAR-8 cell line was provided by Dr. Geeta Mehta (University of Michigan). The OVCAR-8 cell line was tagged with luciferase using retroviral infection with Firefly Luciferase Lentivirus Puromycin (Amsbio, MA). Luc-SKOV-3 and -OVAR-8 cells were cultured in RPMI 1640 1× medium supplemented with 10% FBS (Gibco; Waltham, MA), and 1% penicillin/streptomycin (ThermoFisher Scientific; Waltham, MA).
Firefly luciferase lentivirus puromycin
Manufactured by AMS Biotechnology
The Firefly Luciferase Lentivirus Puromycin is a laboratory tool designed for the expression of the firefly luciferase gene in target cells. It utilizes a lentiviral vector system and incorporates a puromycin resistance marker to enable selection of successfully transduced cells.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 1 medium, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 1x medium, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Pbmcs, by STEMCELL (1 mentions)
2 protocols using firefly luciferase lentivirus puromycin
1
Establishing Ovarian Cancer Cell Lines
2
Ovarian Cancer Cell Lines and CAFs Co-Culture
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The luciferase-tagged SKOV-3 ovarian cancer cell line and the CAFs cell line Human ovarian cancer fibroblast IHFOT-208 were provided by Dr. Michael Birrer (University of Arkansas). The OVCAR-8 cell line was provided by Dr. Geeta Mehta (University of Michigan). The OVCAR-8 cell line was tagged with luciferase using retroviral infection with Firefly Luciferase Lentivirus Puromycin (Amsbio, MA). Luciferase-tagged SKOV-3 and OVAR-8 cells were cultured in RPMI 1640 1X medium, 10% FBS (Gibco; Waltham, MA), and 1% penicillin/streptomycin (ThermoFisher Scientific; Waltham, MA) supplements. IHFOT-208 cells were cultured with DMEM (Gibco, Waltham, MA) 1X with 4.5 g/L glucose, L-glutamine, and sodium pyruvate also supplemented with 10% FBS and 1% penicillin/streptomycin. The CS99 cell line was provided by Gloria Huang (Yale School of Medicine) and was cultured with DMEM (Gibco, Waltham, MA) 1X with 4.5 g/L glucose, L-glutamine, and sodium pyruvate also supplemented with 10% FBS and 1% penicillin/streptomycin. PBMCs were purchased from Stem Cell technologies and were stored in liquid nitrogen until they were used in co-culture with the cancer cells.
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