DOTAP was obtained from Avanti Polar Lipids Inc. (Alabaster, AL, USA). Chol and chondroitin sulfate C sodium salt were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). DOPE was obtained from NOF Corporation (Tokyo, Japan). DXR was purchased from Wako Pure Chemical Industries Inc.
Chondroitin sulfate c sodium salt
Manufactured by Fujifilm
Sourced in Japan
Chondroitin sulfate C sodium salt is a laboratory reagent used in various biochemical and analytical applications. It is a naturally occurring glycosaminoglycan derived from animal sources. The product is primarily used as a structural component in the study of cartilage and connective tissues.
Automatically generated - may contain errors
Lab products found in correlation
Dotap, by Avanti Polar Lipids (1 mentions)
Ammonium persulfate, by Fujifilm (1 mentions)
Span 80, by Tokyo Chemical Industry (1 mentions)
Tween 80, by Tokyo Chemical Industry (1 mentions)
N n dimethylacrylamide, by Fujifilm (1 mentions)
N n dimethylaminoethyl methacrylate, by Fujifilm (1 mentions)
Heparin sodium salt, by Nacalai Tesque (1 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Cholesterol chol, by Fujifilm (1 mentions)
Dna modifying enzymes, by Toyobo (1 mentions)
Chondroitin sulfate c, by Fujifilm (1 mentions)
5 protocols using chondroitin sulfate c sodium salt
1
DOTAP-Based Lipoplex Formulation
2
Synthesis of DMAA-DMAEMA Copolymers
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Monomers, N,N-dimethylacrylamide (DMAA) (049-19185), N,N-dimethylaminoethyl methacrylate (DMAEMA) (044-16276), and the cross-linker N,N′-methylenebisacrylamide (M0506) were obtained from Wako Pure Chemical Industries Ltd. (Osaka, Japan) and Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan), respectively. The stabilizers Span 80 and Tween 80 used during polymerization were purchased from Tokyo Chemical Industry Co., Ltd. and Sigma-Aldrich Co. (St. Louis, USA), respectively. The initiator ammonium persulfate (018-03282) was obtained from Wako Pure Chemical Industries, Ltd. The column (I.D. 5.0 mm, length 10 cm) used for gel packing was obtained from Bio-Rad (Hercules, USA). Sodium glucuronic acid monohydrate and chondroitin sulfate C sodium salt used for adsorption were purchased from Wako Pure Chemical Industries, Ltd. Other chemicals were of analytical grade or higher.
3
Streptobacillus moniliformis Genome and Expression
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Streptobacillus moniliformis DSM 12112 and its genome were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen. Its complete genome sequence is available in GenBank under accession number, CP001779. This bacterium was statically cultured at 37 °C under 5% CO2 in 0.8% nutrient broth (0.3% beef extract and 0.5% peptone) (Difco) containing 20% horse serum (Thermo Fisher Scientific) for 24–48 h. To investigate expression of Smon0127 and Smon0123, S. moniliformis cells were cultured in nutrient broth and horse serum in the presence or absence of 0.2% GAG [hyaluronic acid (Fluka), chondroitin sulfate C sodium salt (Wako), or heparin sodium salt (Nacalai tesque)] dialyzed against pure water. Detailed experimental procedures for construction of the overexpression system of S. moniliformis proteins and protein purification are presented in Supplementary Information.
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Streptobacillus moniliformis DSM 12112 and its genome were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen. Its complete genome sequence is available in GenBank under accession number, CP001779. This bacterium was statically cultured at 37 °C under 5% CO2 in 0.8% nutrient broth (0.3% beef extract and 0.5% peptone) (Difco) containing 20% horse serum (Thermo Fisher Scientific) for 24–48 h. To investigate expression of Smon0127 and Smon0123, S. moniliformis cells were cultured in nutrient broth and horse serum in the presence or absence of 0.2% GAG [hyaluronic acid (Fluka), chondroitin sulfate C sodium salt (Wako), or heparin sodium salt (Nacalai tesque)] dialyzed against pure water. Detailed experimental procedures for construction of the overexpression system of S. moniliformis proteins and protein purification are presented in Supplementary Information.
4
Synthesis and Characterization of DOTAP Lipoplexes
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1,2-Dioleoyl-3-trimethylammonium-propane (DOTAP) methyl sulfate salt was obtained from Avanti Polar Lipids Inc., (Alabaster, AL, USA). Cholesterol (Chol) and chondroitin sulfate C sodium salt were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). All other chemicals were of the finest grade available.
5
Glycosaminoglycan Extraction and Characterization
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Sodium heparin (heparin) was purchased from Nacalai Tesque. Chondroitin sulfate C sodium salt (chondroitin sulfate C) was purchased from Wako Pure Chemical Industries. Hyaluronic acid sodium salt (hyaluronan) was purchased from Sigma-Aldrich. Generally, heparin consists of uronic (glucuronic or iduronic) acid and glucosamine, both of which are linked by the 1,4-glycoside bond and are frequently sulfated and N-acetylated. Chondroitin sulfate C has a repeating disaccharide with the 1,3-glycosidic bond that is composed of glucuronic acid and N-acetyl-d-galactosamine with a sulfate group at the C6 position. Hyaluronan with no sulfate group is composed of glucuronic acid and N-acetyl-d-glucosamine, and shows a similar glycoside bond mode to chondroitin sulfate C. Nutrition-rich media, MRS and GAM, were purchased from BD Biosciences and the Nissui Pharmaceutical Co., respectively. Oligonucleotides were synthesised by Hokkaido System Science (Table S2 ). DNA-modifying enzymes were purchased from Toyobo. All other analytical grade chemicals used in this study were commercially available. Faeces were kind gifts from three Japanese volunteers in their 20’s, 40’s and 50’s. Informed consent was obtained from all subjects.
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