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Uv spectroscopy

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UV spectroscopy is an analytical technique that measures the absorption of ultraviolet (UV) light by a sample. It is used to identify and quantify various compounds in a sample by analyzing the wavelengths and intensities of the absorbed UV light.

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UV-Vis spectroscopy (12 citations) UV-visible spectroscopy (3 citations)

2 protocols using uv spectroscopy

1

Centrifugation-Based CO Entrapment Assessment

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500 mg of CO-SLN was dispersed in 10 mL of distilled water. The aqueous dispersion was centrifuged with 6000 rpm for 20 min at room temperature. The amount of free CO was detected in the supernatant fluid by UV spectroscopy (PerkinElmer, USA) at 270 nm [32 (link)] and the percent of entrapped oil was calculated using the following equation:
EE (%) = (W initial CO-W free CO)/ W initial CO×100
Nemattalab M., Rohani M., Evazalipour M, & Hesari Z. (2022). Formulation of Cinnamon (Cinnamomum verum) oil loaded solid lipid nanoparticles and evaluation of its antibacterial activity against Multi-drug Resistant Escherichia coli. BMC Complementary Medicine and Therapies, 22, 289.
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2

Quantification of SLN Drug Entrapment

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100 mg of SLNs was dissolved in 20 mL of dichloromethane and the solution was centrifuged at 12000 rpm. The supernatant was collected and passed through a membrane filter. The quantity of oil in the solution was measured by UV spectroscopy (PerkinElmer, USA) at 205 nm [30 ]. Entrapment efficacy was calculated using the following formula:
Entrapmentefficiency(%)=Quantityofdruginnanoparticle/Massofdrugintheformulation×100
Nemati S., Mohammad Rahimi H., Hesari Z., Sharifdini M., Jalilzadeh Aghdam N., Mirjalali H, & Zali M.R. (2022). Formulation of Neem oil-loaded solid lipid nanoparticles and evaluation of its anti-Toxoplasma activity. BMC Complementary Medicine and Therapies, 22, 122.
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