Total RNA was isolated from skin tissue or cultured cells and extracted using the Quick-RNA MicroPrep (Zymo Research) according to the manufacturer’s instructions. In total, 1–2 μg of cDNA was synthesized using the high-capacity cDNA reverse transcription kit (Applied Biosystems), and real-time PCR was conducted using an Optical 96 real-time PCR thermal cycler (Biometra). Reactions were run in triplicate, samples were normalized to GAPDH expression, and quantities were determined according to the 2–ΔΔCt method. The primers used for quantitative PCR (qPCR) are listed in Supplemental Table 6 .
Optical 96 real time pcr thermal cycler
Manufactured by Analytik Jena
The Optical 96 is a real-time PCR thermal cycler designed for high-throughput nucleic acid amplification and detection. It features a 96-well format and a precise temperature control system for accurate and reproducible results.
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Lab products found in correlation
2 protocols using optical 96 real time pcr thermal cycler
1
Quantitative PCR Analysis of Gene Expression
2
RNA Isolation and qRT-PCR Analysis
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Total RNA was isolated from skin tissue or cultured cells and extracted using the Quick-RNA™ MicroPrep (Zymo Research) according to the manufacturer’s instructions. A total of 1-2 μg of cDNA was synthesized using the high-capacity cDNA reverse transcription kit (Applied Biosystems), and real-time PCR was conducted using an Optical 96 real-time PCR thermal cycler (Biometra). Reactions were run in triplicate, and samples were normalized to GAPDH expression and quantities determined according to the 2-ΔΔCt method. The primers used for qRT-PCR are listed in Supplementary Table 3
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