Plc prf 5

The Hep3B, Huh7, PLC/PRF/5, and SNU449 HCC cell lines were purchased from the Korean Cell Line Bank (Seoul, Korea). Huh7.5 cells were kindly provided by Dr. Marc Windisch (Institut Pasteur Korea, Gyeonggi-do, Korea), and Huh6 cells were obtained from Cell Bank Australia (Westmead, NSW, Australia). Human immortalized hepatocytes (Fa2N-4) were obtained from XenoTech (Lenexa, KS, USA). All cells were maintained at 37 °C in a humidified atmosphere of 5% CO₂. Dulbecco’s Modified Eagle’s Medium (Welgene, Daegu, Korea) was used to cultivate the Hep3B, Huh7.5, and Huh6 HCC cell lines, and Roswell Park Memorial Institute 1640 (RPMI1640) medium was used to cultivate the Huh7, PLC/PRF/5, and SNU449 HCC cell lines. DMEM and RPMI1640 media were supplemented with heat inactivated 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and 1× penicillin-streptomycin (P/S; Gibco). Fa2N-4 cells were plated on collagen-coated plates (BD Biosciences, San Jose, CA, USA) in serum-containing plating medium (plating media; XenoTech), which was replaced with supporting culture medium (XenoTech) after cell attachment (approximately 3–6 h).

HCC cell lines HepG2, SK-Hep-1, SNU449, and PLC/PRF/5 were purchased from the ATCC. HepG2 and PLC/PRF/5 cells were cultured in Minimum Essential Medium Eagle (MEM; WelGene, Daegu, Korea). SK-Hep-1 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; WelGene). SNU449 cells were cultured in Roswell Park MEMorial Institute Medium 1640 (RPMI-1640; WelGene). All the cell lines, supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, New York, USA) and 1% penicillin/streptomycin (WelGene) were incubated at 37 °C in a 5% CO₂ incubator.