All mice were CD1 males purchased from Charles River. Mice were housed in an AAALAC-accredited facility under 12 h:12 h light/dark cycle, with food and water ad libitum. For EEG/video recording, animals were house individually in 12.5″ × 12.5″ × 15.5″ polycarbonate cages (AAA Plastic Products, Birmingham, AL, USA) with corncob bedding and nesting material. All experiments were conducted in accordance with the NIH Guide for the Care and Use of Laboratory Animals, and with the approval of the Virginia Tech Institutional Animal Care and Use Committee (IACUC; #17-138). Animal work was conducted in accordance with ARRIVE guidelines.
Cd1 males
Manufactured by Charles River Laboratories
Sourced in United States
CD1 males are a common mouse strain used in research applications. They are characterized by their outbred genetic background, which provides a diverse genetic profile. The CD1 males are commonly used as a general-purpose mouse model in various research areas, including toxicology, pharmacology, and developmental studies.
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Lab products found in correlation
6 protocols using cd1 males
1
EEG and Video Monitoring of CD1 Male Mice
2
Social Defeat Stress in Mice
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C57bl6 male (n = 50) and female (n = 28) mice aged 7–8 weeks old and CD1 males aged 4–6 months old were obtained from Charles River Laboratories (USA). In total 50 males and 28 females were subjected to social defeat. Mice were housed under a 12 h light/dark cycle at 22–24 °C with no water and food restriction. CD1 mice were individually housed except during social defeats. All other mice were group-housed (4/cage) before and singly housed after social defeats. Laval’s University Institutional Animal Care Committee, in respect with the Canadian Council on Animal Care guidelines, approved all experimental procedures. The study was carried out in compliance with the ARRIVE guidelines (http://www.nc3rs.org.uk/page.asp?id=1357 ).
3
Chronic Social Defeat Stress in Mice
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C57bl6 male (n = 49) and female (n = 43) mice aged 7-8 weeks old and CD1 males aged 4-6 months old were obtained from Charles River Laboratories (USA). Mice were fed ad libitum at 22-25°C on a 12-hour light/dark cycle. CD-1 mice were singly housed except during social defeats. All other mice were housed in groups (4/cage) before and singly housed after social defeat episodes. In total, 35 males and 30 females were subjected to CSDS. Fourteen males and 13 females served as controls. All experimental procedures were approved by Université Laval's Institutional Animal Care Committee in respect with the Canadian Council on Animal Care guidelines.
4
CD1 Mouse Breeding and Housing
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Eight weeks old female CD1 mice (Charles River, France) were collectively housed under a 12h light/12h dark cycle (lights on from 8 am to 8 pm) in a temperature-(22+/-3°C) and humidity-controlled facility.
Animals had ad libitum access to food and water. After 10 days of habituation to the housing conditions, females were mated with CD1 males (Charles River, France) following a 3 females/1 male breeding design.
Pregnancy was determined by the presence of a vaginal plug checked once a day at the beginning of the light phase. Each female was removed from the breeding couple and individually housed upon plug detection (gestational day GD 0). All procedures and experimental protocols were approved by the Animal Care Committee of Bordeaux (CEEA50) and were conducted in accordance with the European community's council directive of 24 November 1986 (86/609/ECC) that was in effect at the time of the experiments. All efforts were made to minimize animal suffering, and reduce the number of animals used.
Animals had ad libitum access to food and water. After 10 days of habituation to the housing conditions, females were mated with CD1 males (Charles River, France) following a 3 females/1 male breeding design.
Pregnancy was determined by the presence of a vaginal plug checked once a day at the beginning of the light phase. Each female was removed from the breeding couple and individually housed upon plug detection (gestational day GD 0). All procedures and experimental protocols were approved by the Animal Care Committee of Bordeaux (CEEA50) and were conducted in accordance with the European community's council directive of 24 November 1986 (86/609/ECC) that was in effect at the time of the experiments. All efforts were made to minimize animal suffering, and reduce the number of animals used.
5
Social Defeat Stress in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
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C57bl6 male (n = 50) and female (n = 28) mice aged 7-8 weeks old and CD1 males aged 4-6 months old were obtained from Charles River Laboratories (USA). In total 50 males and 28 females were subjected to social defeat. Mice were housed under a 12 hours light/dark cycle at 22-24ºC with no water and food restriction. CD1 mice were individually housed except during social defeats. All other mice were grouphoused (4/cage) before and singly housed after social defeats. All experimental procedures were approved by Laval's University Institutional Animal Care Committee in respect with the Canadian Council on Animal Care guidelines.
6
Social Defeat Stress in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C57bl6 male (n= 50) and female (n=28) mice aged 7-8 weeks old and CD1 males aged 4-6 months old were obtained from Charles River Laboratories (USA). In total 50 males and 28 females were subjected to social defeat. Mice were housed under a 12 hours light/dark cycle at 22-24ºC with no water and food restriction. CD1 mice were individually housed except during social defeats. All other mice were group-housed (4/cage) before and singly housed after social defeats. All experimental procedures were approved by Laval's University Institutional Animal Care Committee in respect with the Canadian Council on Animal Care guidelines.
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