To perform intravital imaging, lipopolysaccharide (LPS) (10 mg/kg body weight; Sigma-Aldrich) dissolved in phosphate-buffered saline (PBS) was injected into the calvarial periosteum of mice under isoflurane anesthesia. To perform RNA sequencing (RNA-Seq), reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and flow cytometry analyses, LPS (20 mg/kg body weight) dissolved in PBS was injected into the calvarial periosteum. From the day of LPS injection, the JAK inhibitor ABT-317, which was provided by AbbVie Bioresearch Center, at a dose of 60 mg/kg body weight dissolved in 0.5% hydroxypropyl methylcellulose (Alfa Aeser) and 0.02% Tween 80 (Sigma-Aldrich) in sterile water was orally administered twice daily for 5 days. To visualize osteoclastic bone resorption using intravital bone imaging, a pH-activatable fluorescent probe for osteoclast activity sensing (pHocas)-3 dissolved in PBS was subcutaneously injected at a dose of 6 mg/kg body weight daily into TRAP-tdTomato mice, beginning 3 days before imaging. The C–C motif chemokine receptor 1 (CCR1) antagonist, J-113863 (MedChemExpress, #HY-103360) (3 mg/kg body weight) dissolved in 10% dimethyl sulfoxide (Sigma-Aldrich), 40% polyethylene glycol 300 (Wako), 5% Tween 80 (Sigma-Aldrich), and 45% PBS, was injected intraperitoneally once daily for 5 days.
Hydroxypropyl methylcellulose
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Hydroxypropyl methylcellulose is a water-soluble polymer used as a viscosity-increasing agent in various applications, including pharmaceutical and food industries. It is a cellulose derivative that provides thickening, stabilizing, and binding properties to formulations.
Automatically generated - may contain errors
Lab products found in correlation
Glycerol, by Thermo Fisher Scientific (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Poloxamer 188, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Tween 80, by Merck Group (1 mentions)
Polyethylene glycol 300, by Fujifilm (1 mentions)
J 113863, by MedChemExpress (1 mentions)
Pluronic f 127, by Merck Group (1 mentions)
Peg400, by Thermo Fisher Scientific (1 mentions)
Cellulose membrane, by Merck Group (1 mentions)
Polyethylene glycol, by Thermo Fisher Scientific (1 mentions)
Carboxy methylcellulose sodium, by Thermo Fisher Scientific (1 mentions)
Curcumin, by Tokyo Chemical Industry (1 mentions)
Ethyl cellulose, by Colorcon (1 mentions)
Dialysis tubing cellulose membrane, by Merck Group (1 mentions)
Hydroxypropyl cellulose, by Thermo Fisher Scientific (1 mentions)
Magnesium stearate, by Colorcon (1 mentions)
Triethylamine tea, by Merck Group (1 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
9 protocols using hydroxypropyl methylcellulose
1
Intravital Imaging of Bone Resorption
2
Curcumin Formulation Optimization
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Curcumin was supplied from TCI (Tokyo Chemical Industry Co., Ltd. Tokyo, Japan). Hydroxy propyl methylcellulose (HPMC), carboxy methylcellulose sodium (Na CMC), glycerol (GLY), polyethylene glycol (PEG 400), triethylacetate 99% (TEC) were supplied by Alfa Aesar, Heysham, England. Polyvinyl alcohol (PVA) molecular weight (MW) 31kDa–50 kDa, 98–99% hydrolysed, Pluronic F127 and cellulose membrane MW cut-off 12 kDa–14 kDa were obtained from Sigma-Aldrich (St. Louis, MO, USA).
3
Cyanocobalamin Buccal Delivery Formulation
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Cyanocobalamin was donated by Planet Pharma, Eudragit (S100, L100, and L100-55) by Evonik Industries, and Mannitol by Pharmacare PLC. Hydroxy propyl cellulose (M.W. 100,000) and hydroxy propyl methyl cellulose ((2% aq. Soln., 20 °C) 7500–14,000 mPa.s) were purchased from Alfa Aesar (Haverhill, MA, USA); ethyl cellulose, microcrystalline cellulose PH 101, polyplasedone, and magnesium stearate from Colorcon® (Milan, Italy); sublingual bovine mucosa from a local butcher (Palestine); PermeaPad® membrane from innoME GmbH (Espelkamp, Germany); dialysis tubing cellulose membrane and polyvinyl pyrrolidine (MW 40,000) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Xanthan gum, carbopol, sodium hydroxide pellets, sodium chloride, disodium hydrogen phosphate, potassium dihydrogen phosphate, potassium chloride, absolute anhydrous ethanol 100%, acetonitrile, and hydrochloric acid 37% were obtained from Birzeit University laboratories (Ramallah, Palestine).
4
Formulation of APX and CLOP Tablets
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A pharmaceutical grade APX and CLOP hydrogen sulfate salt were received as gifts from Pharmacare PLC (Palestine). Tablet excipients including hydroxypropyl methylcellulose, 28–30 % methoxyl, 7–12 % hydroxypropyl, viscosity (2 % aq. soln., 20 °C) 7500–14000 mPa.s (HPMC) and hydroxypropyl cellulose (HPC) were purchased from Alfa Aesar (by Thermos Fisher Scientific, United Kingdom). Methocel, sodium laurilsulfate (SLS), spray-dried lactose, mannitol, microcrystalline cellulose 112 (MCC 112), colloidal silica, sodium stearyl fumarate (SSF), magnesium stearate (Mg. stearate), and anhydrous ethanol were donated from Pharmacare PLC (Palestine). For buffer preparation, sodium hydroxide pellets, sodium phosphate tribasic dodecahydrate, 98 %, and hydrochloric acid were analytical grades and purchased from Carlo Erba reagents, Acros organics, and Merck Serono respectively. HPLC grade solvents for analysis including; acetonitrile (ACN), trifluoroacetic acid (TFA), and triethylamine (TEA) were purchased from Merck (Merck Serono Amman, Jordan). Water was obtained by filtration using a cellulose nitrate filter (0.45) micron manufactured by Sartorius stedim biotech company (Jordan). Translucent hard gelatin shell capsules (0 size) were donated from Jerusalem Pharmaceuticals Co. Ltd. (Palestine).
5
Poloxamer-188 Formulation for Diltiazem Delivery
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Poloxamer-188 was purchased from Merck (Darmstadt, Germany). Diltiazem hydrochloride (DLZ), stearyl alcohol (STA), and hydroxypropyl methylcellulose (HPMC) were obtained from Thermo Fisher Scientific (Loughborough, UK). Tildiem® (60 mg tablets) were ordered from Al Ain Pharmacy (Al Ain, UAE). Lecithin was acquired from Lipoid GmbH (Ludwigshafen, Germany). Sodium taurocholate (NaTC) and pepsin were obtained from Sigma Aldrich (St. Louis, MO, USA). Acetonitrile, 2−propanol, n-hexane, isopropyl alcohol, trimethylamine, ammonium dihydrogen phosphate, phosphoric acid, hydrochloric acid, glacial acetic acid, and sodium hydroxide were acquired from Merck (Darmstadt, Germany). All solvents used were of HPLC grade.
6
Formulation and Evaluation of Sildenafil Citrate Tablets
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Sildenafil citrate (SDF) was purchased from Sigma Aldrich (St. Louis, MO, USA). Poloxamer-188® was obtained from Merck (Darmstadt, Germany), hydroxypropyl methylcellulose (HPMC) was ordered from Thermo-Fisher (Kandel, Germany), and magnesium stearate (MGS) was purchased from Fisher Scientific UK Limited (Loughborough, UK). Viagra® (50 mg) tablets (Pfizer, New York, NY, USA) were obtained from a local retail pharmacy (Abu Dhabi, UAE). All solvents used were of analytical or HPLC grade.
7
Comprehensive Formulation Development Protocol
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NAC crystals and NAC powder (80 mesh) were used as provided by Nestle. Candelilla wax (CanW, melting point 68–72 °C), carnauba wax (CW, melting point 82 °C, no. 1 yellow, refined), sorbitan monooleate or Span 80 (saponification value: 145 to 160), formic acid (FA 98%) and acetonitrile (HPLC grade, ≥99.9%) were purchased from Sigma-Aldrich. Rice bran wax (RBW, melting point 79–85 °C) was purchased from Nutley's Kitchen Gardens. Beeswax (BW, DR-101, melting point 62–65 °C) was purchased from Strahl & Pitsch, Inc. Xanthan gum was purchased from TIC Gums. Carboxymethyl cellulose (CMC) was purchased from Sigma (low viscosity, 50–200 mPa s, 4% in water). Dioctyl sulfosuccinate sodium salt or AOT 96% was obtained from VWR. Hydroxypropyl methylcellulose (HPMC) purchased from Fisher (40–60 mPa s, 2% in water), sorbitan monostearate or Span 60 purchased from TCI America (saponification value: 145 to 156), and hexanes were purchased from Fisher (≥98.5%, Certified ACS, Fisher). Corn oil was purchased from a local market. Milli-Q and DI water were used for analyses and experiments, respectively. All chemicals were used as received without any further purification.
8
Formulation and Characterization of Bioadhesive Gels
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Carbopol 974P NF and carrageenan (Gelcarin GP 379) NF were donated by IMCD Ltd. (Sutton, UK); polyethylene glycol 200, sodium alginate W201502, mucin from porcine stomach, urea, gelatin, and calcium hydroxide were purchased from Sigma-Aldrich (Gillingham, UK). Sodium chloride, potassium hydroxide, lactic acid, glucose, hydroxypropylmethyl cellulose, metronidazole (99%), bovine serum albumin, ethanol, acetic acid, acetonitrile, and glycerol were obtained from Fisher Scientific (Loughborough, UK).
9
Formulation and Characterization of GMO-Based Lipid Nanoparticles
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Two batches of GMO, batch number G1017 (lot numbers ZG0467 and WA1499) were purchased from Spectrum Chemicals; pure GMO was purchased from Sigma-Aldrich. Poloxamer 407 was purchased from Spectrum Chemicals, whereas low molecular weight chitosan and citric acid were purchased from Sigma-Aldrich. Povidone K 90 (polyvinyl pyrrolidone) was purchased from BASF Chemicals. Polyethylene glycols (PEG), Eudragit E100, Eudragit L100, Eudragit S100, hydroxypropyl methyl cellulose (HPMC), mineral oil, and olive oil purchased from Fisher Scientific. Deionized water was used for all the experiments. Physical mixtures used for comparative analysis were prepared by geometrically mixing the lipids/excipients in the same amount as utilized in the preparation of solid lipid nanoparticles.
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