Deta nonoate

Manufactured by Abcam

Sourced in United Kingdom

DETA-NONOate is a nitric oxide (NO) donor compound. It is a stable, water-soluble molecule that can be used to generate controlled concentrations of NO in biological systems.

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Lab products found in correlation

3 protocols using deta nonoate

Characterization of Protein Thiol Modifications

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MG132, CHX, CQ, sodium ascorbate, methyl-methanethiosulfonate (MMTS), biotin-maleimide, dithiothreitol (DTT), GSNO, streptavidin-agarose, neocuproine, dexamethasone, insulin, HEPES, EGTA, collagenase I, and NaHCO₃ were from Sigma-Aldrich. APAP was from MedChemExpress. N6022 was from Selleckchem. DETA-NONOate and isopropyl β-D-1-thiogalactopyranoside (IPTG) were from Abcam. APAP-Cys and APAP-Glu were from Toronto Research Chemicals.

Cui Q., Jiang T., Xie X., Wang H., Qian L., Cheng Y., Li Q., Lu T., Yao Q., Liu J., Lai B., Chen C., Xiao L, & Wang N. (2024). S-nitrosylation attenuates pregnane X receptor hyperactivity and acetaminophen-induced liver injury. JCI Insight, 9(2), e172632.

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Molecular Mechanisms of Inflammatory Bowel Disease

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DETA-NONOate was obtained from Abcam (Cambridge, United Kingdom). Antibodies to A20, cleaved-caspase 3, iNOS, and PARP were obtained from Cell Signaling Technologies (Danvers, MA). GAPDH was obtained from Millipore Sigma (Burlington, MA). p53 was obtained from Santa Cruz Biotechnology (Dallas, TX). Anti-rabbit Cy3 was obtained from Jackson ImmunoResearch (West Grove, PA). DAPI and Phalloidin were obtained from Thermo Fisher Scientific and Abcam, respectively. Recombinant mTNF was obtained from R&D Systems (Minneapolis, MN). Cycloheximide was obtained from Sigma-Aldrich (St. Louis, MO). Cell Titer Glo was obtained from Promega (Madison, WI). DSS 40 kDa was obtained from Chem-Impex Int’l INC (Wood Dale, IL). AOM was obtained from Sigma (St. Louis, MO).

Basta D.W., Vong M., Beshimova A., Nakamura B.N., Rusu I., Kattah M.G, & Shao L. (2022). A20 Restricts NOS2 Expression and Intestinal Tumorigenesis in a Mouse Model of Colitis-Associated Cancer. Gastro hep advances, 2(1), 96-107.

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Pharmacological Manipulation of Memory

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MK-801 (Hello Bio Inc., 304 Wall Street, Princeton, NJ 08540, USA) and scopolamine (Tocris Bioscience/Biotechne Corporation, Minneapolis, MN, USA) were dissolved in 0.9% saline. Spermine NONOate and DETA NONOate (Abcam, Cambridge, UK) were dissolved in 0.9% saline, and NPLA was dissolved in a small amount of DMSO and then adjusted to the proper volume with 0.9% saline. When the administration of experimental compounds was omitted (control, MK-801, or scopolamine group), the animals received appropriate vehicles. The doses used in behavioural experiments were based on our previous studies [8 (link)].

Cieślik P., Borska M, & Wierońska J.M. (2023). A Comparative Study of the Impact of NO-Related Agents on MK-801- or Scopolamine-Induced Cognitive Impairments in the Morris Water Maze. Brain Sciences, 13(3), 410.

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