Six cell lines were acquired for these experiments. Human oesophageal cell line HET-1A (ATCC® CRL-2692™) was used for the healthy control. The Barrett’s oesophagus in vitro model comprised of one non-dysplastic cell line and three high-grade dysplastic cell lines: CP-A/KR-42421 (ATCC® CRL-4027™), CP-B/ CP-52731 (ATCC® CRL-4028™), CP-C/CP-94251 (ATCC® CRL-4029™) and CP-D/CP-18821 (ATCC® CRL-4030™), respectively. The cell line OE19/JROECL19 (ECACC 96071721) was used as a representative for oesophageal adenocarcinoma. All cell lines were authenticated and confirmed negative for mycoplasma infection.
Cp c cp 94251
Manufactured by American Type Culture Collection
The CP-C/CP-94251 is a laboratory incubator designed for the controlled cultivation of cells, tissues, and microorganisms. It maintains a stable temperature, humidity, and atmospheric conditions to support the growth and development of various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Human recombinant epidermal growth factor, by Thermo Fisher Scientific (2 mentions)
Soybean trypsin inhibitor, by Merck Group (2 mentions)
Human recombinant epidermal growth factor, by Merck Group (2 mentions)
Bovine pituitary extract, by Thermo Fisher Scientific (2 mentions)
Cholera toxin, by Merck Group (2 mentions)
Its supplement, by Merck Group (2 mentions)
Adenine, by Merck Group (2 mentions)
Keratinocyte sfm medium, by Thermo Fisher Scientific (2 mentions)
Bovine pituitary extract, by Merck Group (2 mentions)
Het 1a, by American Type Culture Collection (1 mentions)
Balb c nude mice, by Charles River Laboratories (1 mentions)
Hydrocortisone, by Merck Group (1 mentions)
Mcdb153 medium, by Merck Group (1 mentions)
4 protocols using cp c cp 94251
1
Oesophageal Cell Line Characterization
2
BALB/C Nude Mice Esophageal Cancer Model
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A total of SPF-graded 30 BALB/C nude mice (Vital River Laboratories, SCXK (Shanghai) 2017–0011), aged 6–8 weeks and weighing 16–19 g, were housed for 7 days with humidity of 60–80% at temperature of 22 ± 2°C, with freedom to eat and drink, in the condition of light exposure and avoidance alternately for 12 h, respectively. All animal experiments were conducted under the approval of Ethics Committee of our hospital and in accordance to the Guide for the Care and Use of Laboratory Animal. Extensive efforts were made to ensure minimal suffering of the animals included in the study. Human esophageal cancer cell CP-C (CP-94251) was purchased from ATCC and the item number was ATCCCRL-4029.
3
Cell Culture Protocols for CP and EPC2-hTert Lines
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We cultured CP-A (KR-42421), CP-B (CP-52731), and CP-C (CP-94251) cells (American Type Culture Collection, Manassas, VA) in MCDB-153 medium (cat. no. M7403; Sigma-Aldrich, St. Louis, MO) supplemented with 0.4 μg/mL hydrocortisone, 20 ng/mL recombinant human epidermal growth factor (cat. no. E9644; Sigma-Aldrich), 8.4 μg/L cholera toxin (cat. no. H0135; Sigma-Aldrich), 20 mg/L adenine (cat. no. A2786; Sigma-Aldrich), 140 μg/mL bovine pituitary extract (cat. no. P1476; Sigma-Aldrich), ITS supplement (cat. no. I1884; Sigma-Aldrich) (final concentrations: 5 μg/mL insulin, 5 μg/mL transferrin, 5 ng/mL sodium selenite), 4 mM glutamine, and 5% fetal bovine serum. EPC2-hTert cells20 (link) were cultured in Keratinocyte-SFM medium (GIBCO/Life Technologies, Grand Island, NY) supplemented with bovine pituitary extract and recombinant human epidermal growth factor (GIBCO). Soybean trypsin inhibitor (Sigma-Aldrich) was used to quench trypsin activity during cell passage.
4
Cell Culture Protocols for Human Epithelial Cells
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