Rifampicin

Cell culture flasks were obtained from Greiner (Frickenhausen, Germany). Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal calf serum (FCS) were purchased from PAN-Biotech (Aidenbach, Germany). Phosphate-buffered saline (PBS), medium supplements for LS180 cell culture (glutamine, non-essential amino acids, penicillin/streptomycin), penicillin–streptomycin (100×), and zosuquidar were purchased from Sigma-Aldrich (Taufkirchen, Germany). Rifampicin was from Applichem (Darmstadt, Germany), rifabutin was purchased Toronto Research Chemicals (North York, Canada). Rhodamine 123 was purchased from CalBiochem (Darmstadt, Germany). The Absolute QPCR SYBR Green Mix was supplied by Abgene (Hamburg, Germany).

Rifampicin was purchased from AppliChem. Bromopyruvic acid, dexamethasone, gentamicin, staurosporine, and thalidomide were purchased from Sigma-Aldrich. Cyclosporin A, doramapimod, moxifloxacin, necrostatin-1, necrostatin-5, and tacrolimus (FK506) were obtained from Cayman Chemicals. BMS-582949, pamapimod, and Z-VAD-FMK were purchased from Selleckchem. Puromycin dihydrochloride was from Carl Roth (Germany). GR agonist BI653048 was provided by the opnMe platform (Boehringer Ingelheim). The Prestwick chemical library was used for high-throughput screening.

Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal calf serum (FCS) were purchased from PAN-Biotech (Aidenbach, Germany). Phosphate-buffered saline (PBS) and medium supplements (glutamine, non-essential amino acids, penicillin/streptomycin) were purchased from Sigma-Aldrich (Taufkirchen, Germany). Rifampicin, crystal violet, and dimethylsulfoxide (DMSO) were purchased from Applichem (Darmstadt, Germany) and methanol from Roth (Karlsruhe, Germany). Dovitinib was provided by Sequoia Research Products (Pangbourne, UK). Triptolide was purchased from Santa Cruz Biotechnology (Heidelberg, Germany). Oxaliplatin (dissolved in distilled water) was supplied by the University Hospital’s pharmacy. The Dual-Glo Luciferase Assay System, the pGL4.21 vector, the pGL4.74 [hRluc/TK] Renilla vector, and the FuGene® HD Transfection reagent were purchased from Promega Corporation (Madison, WI, USA). The NR1I2 (NM_003889) human cDNA TrueClone® (pCMV6-XL4 vector, containing the cDNA of the PXR gene NR1I2) was obtained from OriGene (Rockville, MD, USA). Cell culture flasks and white 96-well plates with a white bottom (especially well-suited for luminescence measurements) were obtained from Greiner (Frickenhausen, Germany). The luminescence signal was detected with the SpectraMax iD3 from Molecular Devices (Wokingham, UK).