Lipopolysaccharide lps

Manufactured by Biosharp

Sourced in China

Lipopolysaccharide (LPS) is a complex molecule found in the outer membrane of Gram-negative bacteria. It plays a crucial role in the structural integrity and function of these bacterial cells. LPS consists of a lipid component known as lipid A and a polysaccharide component. This molecule is an essential component for various research and laboratory applications involving Gram-negative bacteria.

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LPS (12 citations)

2 protocols using lipopolysaccharide lps

Comprehensive Antibody Panel for Cellular Signaling

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The following antibodies were used: anti-B7-H3 (Proteintech #66 481–1-Ig); antiphospho-p38 MAPK (Thr180/Tyr182) (CST #4511); anti-p38 (CST #9212); antiphospho-eIF4E (S209) (HUABIO #ET1608-66); anti-eIF4E (Proteintech #66 655–1-Ig); anti-HER2 (Proteintech #18 299–1-AP); anti-α-tubulin (Beyotime Biotechnology #AF5012); anti-β-actin (Proteintech #6009–1-Ig); anti-PDL1 (CSB-MA878942A1 m); anti-EGFR (ZENBIO# 201012); HRP conjugated goat antirabbit IgG goat polyclonal antibody (HUABIO #HA1001); and HRP-conjugated goat antimouse IgG goat polyclonal antibody. Antibodies for flow cytometry, including PerCP-Cy5.5-antimouse CD45, FITC-antimouse CD11B, APC-antimouse B7-H3, APC-Cy7-antimouse CD3, FITC-antimouse CD4, BV510-antimouse CD8, APC-antimouse F4-80, BV421-antimouse CD86, PE-antimouse CD206, and PE-antihuman B7-H3, were all purchased from Biolegend.
Cells were treated with the following reagents: SB203580 (Beyotime Biotechnology# S1863); recombinant human TNF-alpha protein (Sinobiological# 10602-HNAE); and lipopolysaccharide (LPS) (Biosharp#BS904).

Zhao S., Wang Y., Yang N., Mu M., Wu Z., Li H., Tang X., Zhong K., Zhang Z., Huang C., Cao T., Zheng M., Wang G., Nie C., Yang H., Guo G., Zhou L., Zheng X, & Tong A. (2022). Genome-scale CRISPR–Cas9 screen reveals novel regulators of B7-H3 in tumor cells. Journal for Immunotherapy of Cancer, 10(6), e004875.

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Schisandrin B-based Nanoparticle Therapy

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Schisandrin B (SchB), N-succinimidyl palmitate (PA-NHSE) and D-galactosamine were obtained from Yuanye Bio-Technology Co., Ltd (Shanghai, China). Tieling Beiya pharmaceutical Oil Co., Ltd (Liaoning, China) provided medium chain triglyceride (MCT). Lipopolysaccharide (LPS) is from Biosharp (Hefei, China), while 1,19 -dioctadecyl- 3,3,39,39 tetramethylindodicarbocyanine (DiD) from Biotium (Hayward, USA). ELISA kits used for assay of the levels of TNF-α, Il-6 and IL-1β. FITC-conjugated anti-rat F4/80 antibody and PE-conjugated anti-rat CD86 antibody were supplied by Invitrogen (CA, USA). Shanghai Bihe Biochemical Technology Co., Ltd offered an anti-CD204 (SR-A) antibody. The anti-CD86 antibody was from Abcam Shanghai Trading Co., Ltd. DAPI was from Beijing Solarbio Science & Technology Co., Ltd. Primary antibodies NFκB-p65, IκBα, phosphor-IκBα (p-IκBα), β-actin and secondary antibodies were from HuaBio Biotechnology Co., Ltd (Zhejiang, China), and phosphorylated NF-κB (p-p65) was from ImmunoWay Biotechnology Co., Ltd (DE, USA). All agents were of analytical grade or better.

Zhang R., Luo S., Zhao T., Wu M., Huang L., Zhang L., Huang Y., Gao H., Sun X., Gong T, & Zhang Z. (2023). Scavenger receptor A-mediated nanoparticles target M1 macrophages for acute liver injury. Asian Journal of Pharmaceutical Sciences, 18(3), 100813.

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