Copper(II) chloride (CuCl2), hydrogen peroxide (H2O2, ~ 30%), Tris base, Pluronic® 127, dopamine, 3,3’,5,5’-tetramethylbenzidine (TMB), and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ammonium hydroxide (NH4OH), ethanol (~ 99%), acetone (~ 99%), and mesitylene were purchased from J.T. Baker® (Pennsylvania, USA) and Alfa Aesar (Heysham, Lancashire) respectively. Cell culture-related products, including Dulbecco’s Modified Eagle Medium (DMEM, CAT: CC103-0500), RPMI 1640 medium (CAT: CC110-0500), and agarose, were obtained from GeneDireX, Inc. Penicillin-streptomycin (10,000 U/mL, CAT: 154140-122) and fetal bovine serum (FBS, CAT: 10437-028) were procured from Gibco®. Interleukin-2 (IL-2, CAT: 50,792-M08H) was procured from Sino Biological.
Agarose
Manufactured by GeneDireX
Sourced in China, United States
Agarose is a polysaccharide derived from red seaweeds. It is commonly used as a gel matrix in various laboratory techniques, such as gel electrophoresis, for the separation and analysis of biomolecules like DNA, RNA, and proteins. Agarose gels provide a porous medium that allows the migration and separation of these molecules based on their size and charge.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Copper 2 chloride, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
3 3 5 5 tetramethylbenzidine (tmb), by Merck Group (1 mentions)
Acetone, by Avantor (1 mentions)
Dopamine, by Merck Group (1 mentions)
Pluronic 127, by Merck Group (1 mentions)
Dna ladder, by GeneDireX (1 mentions)
Nanodrop, by Thermo Fisher Scientific (1 mentions)
Qiamp dna mini kit, by Qiagen (1 mentions)
3 protocols using agarose
1
Copper-Catalyzed Oxidation Assay Protocol
2
Microbiological Media and Chemical Reagents
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All the microbiological media and antibiotic discs were purchased from HiMedia Pvt. Ltd., Co., Mumbai, India. Other chemicals were purchased as primer (integrated DNA technology), Master mix (Takara Bio Inc., Nojihigashi, Japan), DNA Ladder (GeneDirex, Inc., MD, USA), Good view nucleic acid stain- HGV II (SBS Genetech Co., Ltd, Beijing, China), agarose (GeneDireX, Inc.).
3
DNA Isolation from Blood Samples
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A total of 13 DNA samples were isolated from the peripheral blood of volunteers. Three samples (C1, C2 and C3) were used in the tetra-primer ARMS PCR optimization step, while 10 samples (S1-S10) were used in the reproducibility step. QIAmp DNA Mini Kit (50) No. 51304 (Qiagen, United States) was used to isolate the DNA. The procedures followed the instructions of the kit. DNA concentration was checked using a spectrophotometer: Nan-oDrop (Thermo Fisher Scientific, USA). DNA quality and intactness were checked in two percent gel Agarose electrophoresis (Agarose: GeneDireX, USA).
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