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Ap conjugated goat anti human κ l chain ab

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AP)-conjugated goat anti-human κ L chain Ab is a laboratory reagent used to detect and quantify human kappa light chain in biological samples. It is a polyclonal antibody conjugated to alkaline phosphatase (AP) enzyme, which allows for colorimetric or chemiluminescent detection of the target protein.

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Lab products found in correlation

Phosphatase substrate, by Merck Group (2 mentions) Eia ria plate, by Corning (2 mentions) Sunrise plate reader, by Tecan (2 mentions) Diethanolamine, by Merck Group (2 mentions)

2 protocols using ap conjugated goat anti human κ l chain ab

1

Enzyme Immunoassay for Antibody Binding

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Ninety-six-well enzyme immunoassay (EIA)/RIA plates (CorningCostar) were coated overnight (4°C) with Ag (1 mg/ml in PBS) and blocked with PBS/4% skimmed milk powder (S) (Acumedia)/0.05% Tween 20 (T) (Sigma-Aldrich) (PBS/S/T). Titration series of the mAb 26.4 variants (1.0 to 0.008 mg/ml) in PBS/S/T were added for 1 h at room temperature (RT). Bound Abs were detected using an alkaline phosphatase (AP)-conjugated goat anti-human κ L chain Ab (1:5000; Sigma-Aldrich) and visualized by addition of phosphatase substrate (10 mg/ml in diethanolamine) (Sigma-Aldrich) before absorbance values (405 nm) were recorded using a Sunrise plate reader (TECAN). PBS/T was used as washing buffer in between each step.
Mørtberg T.V., Zhi H., Vidarsson G., Foss S., Lissenberg-Thunnissen S., Wuhrer M., Michaelsen T.E., Skogen B., Stuge T.B., Andersen J.T., Newman P.J, & Ahlen M.T. (2022). Prevention of Fetal/Neonatal Alloimmune Thrombocytopenia in Mice: Biochemical and Cell Biological Characterization of Isoforms of a Human Monoclonal Antibody. ImmunoHorizons, 6(1), 90-103.
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2

Enzyme Immunoassay for Antibody Binding

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ninety-six-well enzyme immunoassay (EIA)/RIA plates (CorningCostar) were coated overnight (4°C) with Ag (1 mg/ml in PBS) and blocked with PBS/4% skimmed milk powder (S) (Acumedia)/0.05% Tween 20 (T) (Sigma-Aldrich) (PBS/S/T). Titration series of the mAb 26.4 variants (1.0 to 0.008 mg/ml) in PBS/S/T were added for 1 h at room temperature (RT). Bound Abs were detected using an alkaline phosphatase (AP)-conjugated goat anti-human κ L chain Ab (1:5000; Sigma-Aldrich) and visualized by addition of phosphatase substrate (10 mg/ml in diethanolamine) (Sigma-Aldrich) before absorbance values (405 nm) were recorded using a Sunrise plate reader (TECAN). PBS/T was used as washing buffer in between each step.
Chen X., Ansai T., Awano S., Iida T., Barik S, & Takehara T. (1999). Isolation, Cloning, and Expression of an Acid Phosphatase Containing Phosphotyrosyl Phosphatase Activity from Prevotella intermedia. Journal of Bacteriology, 181(22), 7107-7114.
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