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Anti active caspase 3 pe clone c92 605

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Anti-active caspase-3–PE (clone C92-605) is a fluorescently-labeled antibody that specifically binds to the active form of caspase-3, a key enzyme involved in the execution phase of cell apoptosis (programmed cell death).

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Lab products found in correlation

Rosiglitazone, by Merck Group (3 mentions) Anti cd3 clone okt3, by Thermo Fisher Scientific (3 mentions) Anti cd69 fitc clone l78, by BD (3 mentions) Anti cd134 bv711 clone act35, by BD (3 mentions) Fenofibrate, by Merck Group (3 mentions) Cytofix cytoperm fixation permeabilization solution kit, by BD (3 mentions)

3 protocols using anti active caspase 3 pe clone c92 605

1

Anti-CD3 Stimulation and PPAR Modulation

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PBMCs were stimulated for 24 hr with plate-bound anti-CD3 (clone OKT3; Thermo Fisher Scientific) in the absence or presence of fenofibrate (50 μM; Sigma-Aldrich) or rosiglitazone (40 μM; Sigma-Aldrich). Activation markers were quantified on the cell surface using anti-CD69–FITC (clone L78; BD Biosciences) and anti-CD134–BV711 (clone ACT35; BD Biosciences). Intracellular stains were performed using anti-active caspase-3–PE (clone C92-605; BD Biosciences) in conjunction with a Cytofix/Cytoperm Fixation/Permeabilization Solution Kit (BD Biosciences).
Nicoli F., Cabral-Piccin M.P., Papagno L., Gallerani E., Fusaro M., Folcher V., Dubois M., Clave E., Vallet H., Frere J.J., Gostick E., Llewellyn-Lacey S., Price D.A., Toubert A., Dupré L., Boddaert J., Caputo A., Gavioli R, & Appay V. (2022). Altered basal lipid metabolism underlies the functional impairment of naive CD8+ T cells in elderly humans. Journal of immunology (Baltimore, Md. : 1950), 208(3), 562-570.
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2

Anti-CD3 Stimulation and PPAR Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs were stimulated for 24 hr with plate-bound anti-CD3 (clone OKT3; Thermo Fisher Scientific) in the absence or presence of fenofibrate (50 μM; Sigma-Aldrich) or rosiglitazone (40 μM; Sigma-Aldrich). Activation markers were quantified on the cell surface using anti-CD69–FITC (clone L78; BD Biosciences) and anti-CD134–BV711 (clone ACT35; BD Biosciences). Intracellular stains were performed using anti-active caspase-3–PE (clone C92-605; BD Biosciences) in conjunction with a Cytofix/Cytoperm Fixation/Permeabilization Solution Kit (BD Biosciences).
Nicoli F., Cabral-Piccin M.P., Papagno L., Gallerani E., Fusaro M., Folcher V., Dubois M., Clave E., Vallet H., Frere J.J., Gostick E., Llewellyn-Lacey S., Price D.A., Toubert A., Dupré L., Boddaert J., Caputo A., Gavioli R, & Appay V. (2022). Altered basal lipid metabolism underlies the functional impairment of naive CD8+ T cells in elderly humans. Journal of immunology (Baltimore, Md. : 1950), 208(3), 562-570.
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3

Activation Markers and Apoptosis in PBMCs

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PBMCs were stimulated for 24 hr with plate-bound anti-CD3 (clone OKT3; Thermo Fisher Scientific) in the absence or presence of fenofibrate (50 μM; Sigma-Aldrich) or rosiglitazone (40 Sigma-Aldrich). The expression of the standard activation markers CD69 and CD134 (OX40) was quantified on the cell surface using anti-CD69-FITC (clone L78; BD Biosciences) and anti-CD134-BV711 (clone ACT35; BD Biosciences). Intracellular staining for activated caspase-3, used as marker of susceptibility to apoptosis upon activation, was performed using anti-active caspase-3-PE (clone C92-605; BD Biosciences) in conjunction with a Cytofix/Cytoperm Fixation/Permeabilization Solution Kit (BD Biosciences).
Nicoli F., Cabral-Piccin M.P., Papagno L., Gallerani E., Folcher V., Dubois M., Barrett A.J., Vallet H., Frere J.J., Gostick E., Llewellyn‐Lacey S., Price D.A., Toubert A., Boddaert J., Caputo A., Gavioli R., & Appay V. (2020). Altered basal lipid metabolism underlies the functional impairment of naive CD8+ T cells in elderly humans.
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