Inosine monophosphate

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Inosine monophosphate is a chemical compound that serves as an important intermediate in the biosynthesis of purine nucleotides. It is a key component in the metabolic pathways that produce adenosine triphosphate (ATP) and guanosine triphosphate (GTP), which are essential energy-carrying molecules in biological systems.

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Close spelling variants of this product

Inosine (74 citations) Inosine 5’-monophosphate (7 citations) Inosine 5′-monophosphate disodium salt hydrate (4 citations) Inosine 5′-monophosphate disodium salt (3 citations) Disodium inosine 5′-monophosphate (2 citations) Inosine 5′-monophosphate (IMP) (2 citations)

6 protocols using inosine monophosphate

Extraction and Analysis of Purine Nucleotides

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Acetonitrile, ammonium acetate, methanol, sodium hydroxide, sodium phosphate monobasic, ammonium hydroxide, and potassium chloride were all purchased from Thermo Fisher (Waltham, MA). Inosine monophosphate (IMP), xanthosine monophosphate (XMP), adenosine monophosphate (AMP), 8-bromo-adenosine 5’-monophosphate (BMP, internal standard), and nicotinamide adenine dinucleotide (NAD) were obtained from Sigma (St. Louis, MO). Dulbecco’s Phosphate Buffered Saline (PBS) was purchased from Invitrogen (Grand Island, NY). Ficoll Hypaque solution (density 1.077g/mL) was obtained from GE Healthcare (Uppsala, Sweden). All chemicals were of reagent grade or better.

Bemer M.J., Risler L.J., Phillips B.R., Wang J., Storer B.E., Sandmaier B.M., Duan H., Raccor B.S., Boeckh M.J, & McCune J.S. (2014). RECIPIENT PRETRANSPLANT INOSINE MONOPHOSPHATE DEHYDROGENASE ACTIVITY IN NONMYELOABLATIVE HCT. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, 20(10), 1544-1552.

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Receptor Ligand Binding Assay

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NECA, CGS 21680, and ZM241385 were purchased from Tocris Biosciences.
[³H]CGS 21680 was purchased from PerkinElmer Corporation.
Rolipram, adenosine, inosine, inosine monophosphate, xanthine, hypoxanthine,
adenosine 5′ [α,β-methylene] diphosphate and dipyridamole
were purchased from Sigma-Aldrich.

Welihinda A.A., Kaur M., Greene K., Zhai Y, & Amento E.P. (2016). The adenosine metabolite inosine is a functional agonist of the adenosine A2A receptor with a unique signaling bias. Cellular signalling, 28(6), 552-560.

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Nucleotide Content Analysis by HPLC

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Nucleotide content was analyzed according to Lee
et al. (1984). The 5 g samples were mixed with 25 mL 0.7 M perchloric
acid and homogenized (Polytron R PT-2500 E; Kinematica, Luzern, Switzerland),
and then centrifuged (2,000×g, 15 min, 0°C), filtered using a
Whatman No. 4. filter paper and the remained pellet was re-extracted under same
condition. The supernatant was adjusted to pH 6.5 through 5 N KOH and placed in
a volumetric flask and adjusted to 100 mL with 0.7 M perchloric acid (pH 6.5,
adjusted with 5 N KOH). The mixture was centrifuged (1,000×g, 10 min,
0°C), the supernatant was filtered through a 0.22 μm syringe
filter and analyzed by high-performance liquid chromatography (HPLC; Agilent
1260 Infinity, Agilent Technologies). The analytical conditions for HPLC
included a Nova-pak C18 column (150×3.9 mm, 4-μm particles;
Waters, Milford, MA, USA) eluting 1% trimethylamine · phosphoric acid (pH
6.5) at a 1.0 mL/min flow rate. Injection volume was 10 μL, and running
time was 30 min. Column temperature was maintained at 40°C, and detection
was monitored at a wavelength of 254 nm. Nucleotide content was determined from
a standard curve obtained using the standards adenosine triphosphate (ATP),
adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosine
monophosphate (IMP), inosine, and hypoxanthine (Hx; Sigma-Aldrich, St. Louis,
MO, USA).

Jung Y., Kim H.J., Kim D., Joo B., Jhoo J.W, & Jang A. (2023). Physicochemical Properties and Volatile Organic Compounds of Dairy Beef Round Subjected to Various Cooking Methods. Food Science of Animal Resources, 43(5), 767-791.

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Quantitative Analysis of Nucleotides

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Adenosine, Adenosine Monophosphate (AMP), Adenosine triphosphate (ATP), Guanosine, Guanosine monophosphate (GMP), guanosine triphosphate (GTP), Inosine, Inosine monophosphate (IMP), and Inosine triphosphate (ITP) were purchased from Sigma Aldrich, St. Louis, MO; isotopic internal standards for each, (Ribose ¹³C₅) were from Cambridge Isotope Laboratories, Andover, MA.
Analytical grade reagents were purchased from Fisher Scientific, Fairlawn, NJ, (acetonitrile, methanol, formic acid, potassium chloride, phosphoric acid, and ammonium hydroxide). Ammonium acetate 5 M solution was purchased from Ambion^® and alkaline phosphatase was purchased from Sigma Aldrich, St. Louis. Ultrapure (UP) water was prepared in house from deionized water with a Barnstead Nanopure System (Thermo Fisher Scientific, Waltham, MA). Other supplies included Waters Sep-Pak Accell Plus QMA Cartridge, 3 cc (500 mg) (Waters Corporation, Milford, MA) and Varian Bond-Elut LRC Phenylboronic Acid (PBA) Cartridge 100mg/10 mL (Agilent, Santa Clara, CA). PAXgene^® tubes were from Qiagen, Valencia, CA.

Jimmerson L.C., Clayton C.W., MaWhinney S., Meissner E.G., Sims Z., Kottilil S, & Kiser J.J. (2016). Effects of ribavirin/sofosbuvir treatment and ITPA phenotype on endogenous purines. Antiviral research, 138, 79-85.

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Quantitative Analysis of Nucleotides

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Adenosine, Adenosine Monophosphate (AMP), Adenosine triphosphate (ATP), Guanosine, Guanosine monophosphate (GMP), guanosine triphosphate (GTP), Inosine, Inosine monophosphate (IMP), and Inosine triphosphate (ITP) were purchased from Sigma Aldrich, St. Louis, MO; isotopic internal standards for each, (Ribose ¹³C₅) were from Cambridge Isotope Laboratories, Andover, MA.
Analytical grade reagents were purchased from Fisher Scientific, Fairlawn, NJ, (acetonitrile, methanol, formic acid, potassium chloride, phosphoric acid, and ammonium hydroxide) as well as Whatman^® 903 DBS cards, bags and desiccant for DBS preparation and storage. Ammonium acetate 5M solution was purchased from Ambion® and alkaline phosphatase was purchased from Sigma Aldrich, St. Louis. Ultrapure (UP) water was prepared in house from deionized water with a Barnstead Nanopure System (Thermo Fisher Scientific, Waltham, MA). Other supplies included Waters Sep-Pak Accell Plus QMA Cartridge, 3cc (500mg) (Waters Corporation, Milford, MA) and Varian Bond-Elut LRC Phenylboronic Acid (PBA) Cartridge 100mg/10mL (Agilent, Santa Clara, CA). Blood products for lysed cellular matrix were obtained from Bonfils, Denver CO. Cell preparation tubes (CPT) and dipotassium EDTA tubes were purchased from Becton, Dickinson and Company, Franklin Lakes, NJ.

Jimmerson L.C., Bushman L.R., Ray M.L., Anderson P.L, & Kiser J.J. (2016). A LC-MS/MS method for quantifying adenosine, guanosine and inosine nucleotides in human cells. Pharmaceutical research, 34(1), 73-83.

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Crystalline Amino Acids in Krill Hydrolysate Diets

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Crystalline amino acids arginine (Arg), alanine (Ala), proline (Pro), leucine (Leu), phenylalanine (Phe) and nucleotides (AMP, guanosine monophosphate (GMP), cytidine monophosphate (CMP), inosine monophosphate (IMP)) were purchased from Sigma Aldrich (Oslo, Norway) and used singly or mixed in combination at levels similar to those in krill hydrolysate (KH) (Kousoulaki et al., 2013) :
A5) Arg+Ala+Pro+Leu+Phe + nu + rest FAA as in KH Each mix was added to one of five otherwise identical low fishmeal (3%) diets. A 15% fishmeal (MFM) diet and a 3% fishmeal diet (LFM) served as positive and negative control diets, respectively. The experimental diets' formulation, approximate composition and physical quality, in addition to their total and FAA composition, are presented in Tables 1-3.

Kousoulaki K., Rønnestad I., Rathore R., Sixten H.J., Campbell P., Nordrum S., Berge R.K, & Albrektsen S. (2018). Physiological responses of Atlantic salmon (Salmo salar L.) fed very low (3%) fishmeal diets supplemented with feeding-modulating crystalline amino acid mixes as identified in krill hydrolysate. Aquaculture (Amsterdam, Netherlands), 486.

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