Mouse igg a7028

The primary antibodies used in this study were at the dilutions of 1:1000 as follows: SFRS8 (24705‐1‐AP, ProteinTech Group, China; ab72044, Abcam, UK); poly ADP‐ribose polymerase (PARP) (9542S, Cell Signaling Technology, USA); β‐catenin (51067‐1‐AP, ProteinTech Group, China); β‐actin (4970S, Cell Signaling Technology, USA); Alix (2171S, Cell Signaling Technology, USA); CD9 (13174S, Cell Signaling Technology, USA); Calnexin (10427‐2‐AP, ProteinTech Group, China); Ubiquitin (10201‐2‐AP, ProteinTech Group, China); HA (51064‐2‐AP, ProteinTech Group, China); DYKDDDDK (98533S, Cell Signaling Technology, USA). The anti‐DYKDDDDK antibody (101274‐mm05t, Sino Biological, China) was at the 1:50 dilution, and Goat pab to Ms IgG (FITC) (ab6785, Abcam, UK) was at the 1:200 dilution. The second antibodies Goat anti‐Rabbit IgG(H+L) HRP (FMS‐Rb01, Fcmacs) and goat anti‐Mouse IgG (H+L) HRP (S0002, Affinity) were in 5 000 diluted concentrations.
Doxycycline (DOX), rabbit IgG (a7016) and mouse IgG (a7028) were purchased from the Beyotime (Shanghai, China). Puromycin was obtained from Merck KGaA (Darmstadt, Germany). Diphenyltetrazolium Bromide (MTT) was purchased from Solarbio (Shanghai, China). Methyl 3‐{[(4‐methylphenyl) sulfonyl] amino}benzoate (MSAB) was purchased from Selleck (Shanghai, China). GW4869 was purchased from MedChemExpress (Monmouth Junction, NJ, USA).

5′-pppRNA and poly(I:C) were purchased from InvivoGen, and were used at a final concentration of 0.5 µg/mL and 10 µg/mL, respectively. MG132, IFN-β, IFN-β ELISA kit, Glutathione-Sepharose, and the Dual-Luciferase Reporter Assay System were purchased from Sigma Aldrich, R&D Systems, PBL Interferon Source, GE Healthcare, and Promega, respectively. Antibodies against the following IAV proteins were generated in our Laboratory: PB2, PB1, PA, and NP. Anti-TRAF3 (sc-6933), anti-TRIM35 (sc-100880), and anti-ubiquitin (sc-8017) were from Santa Cruz. Anti-TRAF3 (4729), anti-MAVS (24930), anti-K63-linkage specific polyubiquitin (5621), anti-K48-linkage specific polyubiquitin (8081) were from Cell Signaling. Anti-Flag (F1804; F7425), anti-Myc (C3965; M4439), anti-V5 (V8012), and anti-TRIM35 (SAB2103161) were from Sigma Aldrich. Anti-IFITM3 (11714-1-AP), anti-HA (51064-2-AP), and anti-GAPDH (10494-1-AP) were from Proteintech. Anti-V5 (AB3792) was from Millipore. Anti-GFP (ab6556), and anti-GST (ab58626) were from Abcam. Mouse IgG (A7028) was from Beyotime. Alexa Fluor 633 goat anti-mouse IgG (H + L) (A21050) and Alexa Fluor 488 donkey anti-rabbit IgG (H + L) (A21206) from Life Technologies were used for confocal microscopy. DyLight 800 goat anti-mouse IgG (H + L) (072-07-18-06) and DyLight 800 goat anti-rabbit IgG (H + L) (072-07-15-06) were from KPL.