Insulin radioimmunoassay kit

Plasma insulin level (μg/L) was assayed by an insulin radioimmunoassay kit purchased from Linco Research Inc. (St. Charles, MO, USA).

Plasma glucose was analysed using a Trinder reagent kit (Trinder 1969 ). Plasma insulin was measured by insulin radioimmunoassay kit (Bürgi et al. 1988 (link)) (Linco Research, Inc., St. Charles, MO). Total tissue lipid content was determined using a method described by Folch et al. (1957 ). TC was evaluated by a method described by Allain et al. (1974 (link)). High-density lipoprotein-cholesterol (HDL-C) levels were assayed by the Izzo et al. method (Izzo et al. 1981 (link)). Low-density lipoprotein-cholesterol (LDL-C) and very low-density lipoprotein-cholesterol (VLDL-C) levels were determined according to the Friedewald et al. method (Friedewald et al. 1972 (link)). TG concentrations were analysed using the McGowan et al. method (McGowan et al. 1983 (link)). Free fatty acid (FFA) concentrations were determined using the method described by Falholt et al. (1973 ). Phospholipids (PLs) concentrations were analysed using the Silversmit and Davis method (Silversmit and Davis 1950 ).

We examined glucose-stimulated insulin secretion in WT, RYR1-p.R163C, and db/db mice. Plasma insulin concentrations were measured using an insulin radioimmunoassay kit (Linco Research, St. Charles, Mo., USA). Mice fasted for 12 h were IP injected with glucose (2 g/kg body weight) and venous blood was collected at 0-, 30-, 60-, 90-, and 120-min. Glucose-stimulated insulin secretion was expressed as the AUC during the 120-min test duration using the same approach as described in the glucose tolerance test in mice.