Isolated retains were dissociated to a single cell level as described previously.22 (link) Peripheral blood was collected from tail veins, and the erythrocytes were lysed using ACK lysing buffer (Thermo Fisher Scientific). Cells were stained with PE-conjugated anti-CD11b antibody (Bio Legend, San Diego, CA, USA), Alexa Fluor647-conjugated anti-CD73 antibody (BD Biosciences), or Annexin V-PE (Bio Vison, Milpitas, CA, USA). Dead cell exclusion was done by using propidium iodide (Sigma-Aldrich). Cells were analyzed by FACS Calibur (BD Biosciences). Data analysis were performed using Flow Jo software (BD Biosciences).
Alexa fluor647 conjugated anti cd73 antibody
Manufactured by BD
Sourced in United States
Alexa Fluor647-conjugated anti-CD73 antibody is a laboratory reagent used to detect and quantify the presence of the CD73 cell surface protein. The antibody is conjugated to the Alexa Fluor647 fluorescent dye, allowing for visualization and analysis of CD73-expressing cells using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
2 protocols using alexa fluor647 conjugated anti cd73 antibody
1
Isolation and Analysis of Myeloid Cells
2
Isolated Retains Cell Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Isolated retains were dissociated to single cell level as described previously [20] . Peripheral blood were collected from tail vein, and the erythrocytes were lysed using ACK lysing buffer (Thermo Fisher Scienti c). Cells were stained with PE-conjugated anti-CD11b antibody (Bio Legend), Alexa Fluor647conjugated anti-CD73 antibody (BD Biosciences), or Annexin V-PE (Bio Vison). Dead cell exclusion was done by using propidium iodide (Sigma-Aldrich). Cells were analyzed by FACS Calibur (BD Biosciences). Data analysis were performed using Flow Jo software (BD Biosciences).
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