Captisol

Manufactured by MedChemExpress

Captisol is a chemically modified cyclodextrin that is used to improve the solubility, stability, and bioavailability of various pharmaceutical compounds. It functions as a drug delivery excipient, enhancing the solubilization and formulation of poorly soluble drugs.

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Lab products found in correlation

Erlotinib, by Selleck Chemicals (2 mentions) Mk2206, by MedChemExpress (1 mentions) Insulin syringe, by BD (1 mentions) Prexasertib, by MedChemExpress (1 mentions) Hydroxypropyl β cyclodextrin, by Merck Group (1 mentions)

6 protocols using captisol

Lung Metastasis Model and Erlotinib Treatment

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To establish the lung metastasis model, 5- to 6-week old male nude mice (n = 12 per group) were injected with stable PLC-8024 or Huh7 cells (2 × 10⁶ cells suspended in 200 μL PBS) through the tail veins. Fifty days after injection, the mice were sacrificed, and the lungs were harvested for haematoxylin and eosin (H&E) staining, which was used to count the number of metastatic nodes in lung sections.
To assess the effects of erlotinib treatment, after injection with Huh7 stable Elafin overexpressing or vector cells (2 × 10⁶⁾ via the tail veins of the nude mice, the mice were treated with erlotinib (S1023, Selleck Chemicals) in PBS with 10% Captisol (HY-17031, MedChemExpress) at 80 mg/kg orally [26 (link)] or with vehicle (PBS with 10% Captisol) daily from the 4th to 7th week after injection. On day 50, the mice were sacrificed, and the lungs were collected. H&E staining was performed on paraffin-embedded lungs to identify the metastatic nodes in the lungs.
All BALB/c nude mice were purchased from the Medical Experimental Animal Center of Guangdong Province (China) and fed at the Animal Experimental Center of Sun Yat-Sen University. All animal research procedures were performed according to the Animal Care and Use Ethics Committee of Sun Yat-Sen University Cancer Center.

Wang C., Liao Y., He W., Zhang H., Zuo D., Liu W., Yang Z., Qiu J., Yuan Y., Li K., Zhang Y., Wang Y., Shi Y., Qiu Y., Gao S., Yuan Y, & Li B. (2021). Elafin promotes tumour metastasis and attenuates the anti-metastatic effects of erlotinib via binding to EGFR in hepatocellular carcinoma. Journal of Experimental & Clinical Cancer Research : CR, 40, 113.

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Oral MK2206 administration before hypercapnia and IAV

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MK2206 (MedChemExpress LLC) was administrated by oral gavage at 120 mg/kg body weight one day before hypercapnia exposure and then one day before IAV infection. Captisol (MedChemExpress LLC) was used as a vehicle for the drug and control animals were treated with vehicle only.

Casalino-Matsuda S.M., Chen F., Gonzalez-Gonzalez F.J., Nair A., Dib S., Yemelyanov A., Gates K.L., Budinger G.R., Beitel G.J, & Sporn P.H. (2020). Hypercapnia Suppresses Macrophage Antiviral Activity and Increases Mortality of Influenza A Infection via Akt1. Journal of immunology (Baltimore, Md. : 1950), 205(2), 489-501.

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GSK-126 Mouse Xenograft Study

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GSK-126 (Merca Chem) was reconstituted in 20% Captisol (Medchem Express), and brought to a pH of 4.5 with 10 M potassium hydroxide, to create a working stock of 150 mg/ml. Mice were weighed weekly, and administered 300 mg/kg of GSK-126 or an equal volume of vehicle via intraperitoneal injection, three times a week. Tumour growth was assessed in a blinded fashion, with twice weekly caliper measurements. Treatment groups were distributed, such that each cage had at least one mouse receiving each of the treatment conditions.

Hirukawa A., Smith H.W., Zuo D., Dufour C.R., Savage P., Bertos N., Johnson R.M., Bui T., Bourque G., Basik M., Giguère V., Park M, & Muller W.J. (2018). Targeting EZH2 reactivates a breast cancer subtype-specific anti-metastatic transcriptional program. Nature Communications, 9, 2547.

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Spontaneous Spleen-Liver Metastasis Model

Cited 1 time

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The spontaneous spleen–liver metastasis model has been published previously (14 (link)). Male nude mice ages 5 to 6 weeks were anesthetized by continuous inhalation with isoflurane, and 2 × 10⁵ cells in 30 μL DMEM with 33% Matrigel (354230, BD Biosciences) were injected into the spleen of laparotomized mouse using insulin syringes (328438, BD Biosciences). Twenty-eight days after inoculation, the mice were euthanized. The livers and spleens were weighed before the metastatic nodules in livers were counted, and the livers and spleens were also subjected to hematoxylin and eosin (H&E) staining. To assess the effects of erlotinib treatment, 5 × 10⁵ cells were injected, and 15 days after cell inoculation, the mice were treated with erlotinib (S1023, Selleck Chemicals) in PBS with 6% Captisol (HY-17031, Med-ChemExpress) at 50 mg/kg (i.g.) daily for 14 days, and the mice were then euthanized for further assessment.

Zheng L.S., Yang J.P., Cao Y., Peng L.X., Sun R., Xie P., Wang M.Y., Meng D.F., Luo D.H., Zou X., Chen M.Y., Mai H.Q., Guo L., Guo X., Shao J.Y., Huang B.J., Zhang W, & Qian C.N. (2016). SPINK6 Promotes Metastasis of Nasopharyngeal Carcinoma via Binding and Activation of Epithelial Growth Factor Receptor. Cancer research, 77(2), 579-589.

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Combination Chemotherapeutic Regimen Evaluation

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4-HPC was purchased from Toronto Research Chemicals, and GEM from Medchem Express. For in vitro assays, drugs were dissolved in DMSO and stored at −80°C, with the exception of CDDP, which was purchased as a 1 mg/mL solution in saline (Hospira). For in vivo administration, CPM (Endoxan, Baxter) was dissolved in saline and 120 mg/kg delivered intraperitoneally (i.p.) once weekly. CDDP and GEM were diluted in phosphate-buffered saline (PBS) and 3 mg/kg (i.p.) or 60 mg/kg (i.v.), respectively, were delivered once weekly. CHK inhibitors were purchased from Medchem Express except for prexasertib (MedKoo Biosciences). AZD7762 was dissolved in 11% cyclodextrin (Sigma) and 30 mg/kg delivered i.v. (22 (link)), MK8776 was dissolved in 20% hydroxypropyl-β-cyclodextrin (Sigma) and 40 mg/kg delivered i.p., and LY2606368-hydrochloride and prexasertib were dissolved in 20% Captisol (Medchem Express) and 20 mg/kg delivered i.v. once per day, or 10 mg/kg delivered s.c. twice in one day 10–12 h apart. LY2603618 was formulated for daily oral dosing (p.o.) at 200 mg/kg in 16.7% Captisol in 25 mM phosphate buffer, pH 4 (59 (link)). Treatment schedules are shown in figures and were repeated weekly for 6 weeks unless tumor-related morbidity or other ill health was observed.

Endersby R., Whitehouse J., Pribnow A., Kuchibhotla M., Hii H., Carline B., Gande S., Stripay J., Ancliffe M., Howlett M., Schoep T., George C., Andradas C., Dyer P., Schluck M., Patterson B., Tacheva-Gigorova S.K., Cooper M.N., Robinson G., Stewart C., Pfister S.M., Kool M., Milde T., Gajjar A., Johns T., Wechsler-Reya R.J., Roussel M.F, & Gottardo N.G. (2021). Small molecule screen reveals synergy of cell cycle checkpoint kinase inhibitors with DNA-damaging chemotherapies in medulloblastoma. Science translational medicine, 13(577), eaba7401.

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NSG Mice Xenograft Model for Carfilzomib and Cyclosporin A Evaluation

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Either 0.5, 0.7 or 1 × 10⁶ LPS141 cells were inoculated subcutaneously in the flank of 6–8 weeks old NSG (NOD-SCID gamma) mice. DMSO (vehicle control) or carfilzomib diluted in Citrate buffer (25 mM, pH = 4) + 10% Captisol (MedchemExpress) were administered by intraperitoneal injection on alternate days. For combinational treatments, cyclosporin A diluted in 10% DMSO and olive oil was administered by oral gavage on alternate days. At the end of each experiment, mice were sacrificed; tumors were collected and weighed. All experiments were performed in compliance with ethical regulations of Institutional Animal Care and Use Committee (IACUC) of National University of Singapore.

Jeitany M., Prabhu A., Dakle P., Pathak E., Madan V., Kanojia D., Mukundan V., Jiang Y.Y., Landesman Y., Tam W.L., Kappei D, & Koeffler H.P. (2020). Novel carfilzomib-based combinations as potential therapeutic strategies for liposarcomas. Cellular and Molecular Life Sciences, 78(4), 1837-1851.

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