Af 413 na

Manufactured by R&D Systems

AF-413-NA is a recombinant mouse Activin A protein. Activin A is a member of the transforming growth factor-beta (TGF-β) superfamily and plays a role in diverse biological processes.

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Lab products found in correlation

Anti nf κb p65, by Santa Cruz Biotechnology (1 mentions) Anti phospho nf κb p65, by Santa Cruz Biotechnology (1 mentions) Anti histone h3, by Abcam (1 mentions) Il 13, by Thermo Fisher Scientific (1 mentions) 4 phenylbutyric acid 4 pba, by Merck Group (1 mentions) Anti akt1, by Santa Cruz Biotechnology (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti lyn, by Santa Cruz Biotechnology (1 mentions) Anti bip, by Abcam (1 mentions) Anti muc5ac, by Santa Cruz Biotechnology (1 mentions) Anti pi3k p85α, by Santa Cruz Biotechnology (1 mentions) Anti chop, by Abcam (1 mentions) Anti il 13, by Abcam (1 mentions) Anti phospho lyn, by Cell Signaling Technology (1 mentions) Anti il 13, by R&D Systems (1 mentions) Ab 108 c, by R&D Systems (1 mentions) Bm0002, by Boster Bio (1 mentions)

3 protocols using af 413 na

Antibody-Based Histology and Cell Staining Protocols

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The following antibodies for histology and cell staining were purchased from Santa Cruz Biotechnology: anti-MUC5AC (Santa Cruz, CA, sc-16903, AB_649616), anti-phospho-PI3K p85α (Tyr467: Santa Cruz, CA, sc-293115, AB_10844180), anti-PI3K p85α (Santa Cruz, CA, sc-31970, AB_2268186), anti-phospho-Akt1 (Thr308: Santa Cruz, CA, sc-135650, AB_2224730), anti-Akt1 (Santa Cruz, CA, sc-1618, AB_630849), anti-phospho-NFκB p65 (Ser536: Santa Cruz, CA, sc-33020, AB_2179018), anti-NFκB p65 (Santa Cruz, CA, sc-109, AB_632039), anti-Lyn (Santa Cruz, CA, sc-15, AB_2281450), and anti-β-actin (Santa Cruz, CA, sc-130656, AB_2223228). The following antibodies for histology were purchased from Abcam Biotechnology: anti-BIP (Abcam, ab21685, AB_2119834), anti-CHOP (Abcam, ab11419, AB_298023), anti-histone H3 (Abcam, ab1791, AB_302613), and anti-IL-13 (Abcam, ab133353, AB_11157609). Anti-phospho-Lyn (Tyr416: Cell Signaling Technology, #2101, AB_331697) was purchased from Cell Signaling Technology. Anti-IL13 (R&D Systems, AF-413-NA, and AB_2124173) and IL-13 ELISA reagents (R&D Systems, M1300CB) were purchased from R&D Systems. IL-13 (PeproTech, #200-13), 4-Phenylbutyric acid (4-PBA, Sigma-Aldrich, P21005), PI3K Inhibitor PI-103 (Selleck, S1038) were purchased as indicated. A nonsilencing siRNA control and a Lyn-specific siRNA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Wang X., Yang X., Li Y., Wang X., Zhang Y., Dai X., Niu B., Wu J., Yuan X., Xiong A., Liu Z., Zhong N., Wu M, & Li G. (2016). Lyn kinase represses mucus hypersecretion by regulating IL-13-induced endoplasmic reticulum stress in asthma. EBioMedicine, 15, 137-149.

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Differentiation of Bone Marrow Macrophages

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Bone marrow cells from 8- to 12-week-old male or female mice were cultured in DMEM (Corning, catalog 10-013) supplemented with 10% heat-inactivated FBS, 1× of penicillin streptomycin l-glutamine, and 10 μg/mL of macrophage colony-stimulating factor (Miltenyi Biotec, catalog 130-101-706). Adherent monocytes were left to differentiate into macrophages for 5 days with medium change once after 3 days of culture (52 (link)). BMDMs were harvested and plated in 48-well plates (2 × 10⁵ cells per well) a day before coculturing with CD8⁺ T cells isolated from the spleen in a 1:1 ratio. Anti–IL-13 (1 μg/mL; R&D Systems, catalog AF-413-NA) or control IgG (R&D Systems, catalog AB-108-C) were added in some wells. Forty-eight hours later, cells were collected for flow cytometric analysis of intracellular IL-10.

Singh S.K., Krukowski K., Laumet G.O., Weis D., Alexander J.F., Heijnen C.J, & Kavelaars A. (2022). CD8+ T cell–derived IL-13 increases macrophage IL-10 to resolve neuropathic pain. JCI Insight, 7(5), e154194.

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Immunohistochemical Analysis of Mouse Liver

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After sacrifice, the mouse livers were immediately fixed in 4% paraformaldehyde and then paraffin-embedded. Liver sections of 4 μm were prepared. Endogenous peroxidase was blocked with 3% hydrogen peroxide (H₂O₂). Anti-IL-13 (AF-413-NA, R&D Systems), antismooth muscle actin alpha (α-SMA) (BM0002, BOSTER), or anti-tTG (sc-20621, Santa Cruz Biotechnology) primary antibody was diluted in 50-fold. IL-13, α-SMA, and tTG expressions were detected using GTVision II Detection System/Mo&Rb (Gene Tech (Shanghai) Co., Ltd.) according to the manufacturer's instructions.

Tang J., Huang H., Ji X., Zhu X., Li Y., She M., Yan S., Fung M, & Li Z. (2014). Involvement of IL-13 and Tissue Transglutaminase in Liver Granuloma and Fibrosis after Schistosoma japonicum Infection. Mediators of Inflammation, 2014, 753483.

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