Objective lens

Manufactured by Leica

Sourced in Germany

The 40× objective lens is a high-magnification lens designed for use in microscopy applications. It provides a 40x magnification, allowing for detailed examination and analysis of microscopic samples.

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Lab products found in correlation

Alexa fluor 546 conjugated phalloidin, by Thermo Fisher Scientific (5 mentions) Confocal microscope, by Leica (2 mentions) Dm4000 microscope, by Leica (1 mentions) Las v4.5 controller software, by Leica (1 mentions) Dfc450 c camera, by Leica (1 mentions) Invia reflex, by Leica (1 mentions)

Close spelling variants of this product

HC PL APO CS2 20× DRY objective lens HC PL APO 100x/1.40 Oil Ph3 objective lens 40x HCX PL AP 1.10 NA water immersion objective lens Leica 63X oil objective lens 63×/1.4NA Plan Apochromat oil immersion objective lens 100x HCX PL APO 1.4–0.70NA oil objective lens HCX PL APO ×63 oil objective lens HC PL APO CS2 63×/NA1.40 oil objective lens N plan 10 × 0.25 numerical aperture objective lens ×86/1.20 STED WHITE water immersion objective lens

14 protocols using objective lens

In-situ Raman Study of Catalyst

Cited 1 time

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The spectra of powder samples were collected with an Invia Renishaw
Raman microscope equipped with a laser excitation of 532 nm and 1800
l/mm grading and coupled with a 50× objective lens (Leica). In
situ Raman study was done with 785 nm laser, 1200 l/mm grading, 50×
objective lens (Leica) in 0.1 M high-purity semiconductor-grade KOH
in homemade electrochemical cell. Au substrate was polished and roughened
following the previously reported protocol^{69 (link)} and the sample ink solution was dropcasted on to it. The sample
ink solution consisted of 4.8 mg of catalyst, 1 mL of water: isopropanol
(3:1) mixture, and 50 uL of nafion. Pt wire and RHE were used as the
counter and reference electrodes, respectively. Argon saturated electrolyte
was used, and the flux was controlled using a peristaltic pump with
a flow rate of 1–8 mL/min in order to remove the bubble formation.
Before applying bias, the sample was immersed in electrolyte for 30
min to check the solvation effect. In situ Raman spectra were then
collected with fixed potential in the chronoamperometric (CA) mode
from 1.0 to 1.5 V vs RHE.

Cheraparambil H., Vega-Paredes M., Scheu C, & Weidenthaler C. (2024). Unraveling the Evolution of Dynamic Active Sites of LaNixFe1–xO3 Catalysts During OER. ACS Applied Materials & Interfaces, 16(17), 21997-22006.

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Assessing CD33-Mediated F-Actin Induction

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Example 16

The purpose of this Example is to test whether antagonistic anti-CD33, or CD33 bispecific antibodies induce F-actin in microglial cells, macrophages, and dendritic cells.

Microglia, macrophages or dendritic cells and other cells of interest that are transduced with CD33 or that express CD33 are added to culture plates and then exposed to antagonistic anti-CD33 and/or CD33 bispecific antibodies, or a control antibody. Cells are fixed, blocked, and then stained with Alexa Fluor 546-conjugated phalloidin (Molecular Probes) after 1 h and F-actin is labeled with a fluorescence dye. Images are collected by confocal laser scanning microscopy with a 40× objective lens (Leica). (JEM (2005), 201, 647-657).

US11136390B2. Anti-CD33 antibodies and methods of use thereof (2021-10-05). ALECTOR LLC [US]. Inventors: Kate Monroe [US], Helen Lam [US], Francesca Avogadri-Connors [US], Seung-Joo Lee [US], William Monteith [US], Herve Rhinn [US], Arnon Rosenthal [US].

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Siglec-9 Antibody Effects on Immune Cells

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Example 16

The purpose of this Example is to test whether antagonistic anti-Siglec-9 antibodies, or Siglec-9 bispecific antibodies induce F-actin in microglial cells, macrophages, neutrophils, NK cells, and dendritic cells.

Microglia, macrophages, neutrophils, NK cells, or dendritic cells and other cells of interest that are transduced with Siglec-9 or that express Siglec-9 are added to culture plates and then exposed to antagonistic anti-Siglec-9 and/or Siglec-9 bispecific antibodies, or a control antibody. Cells are fixed, blocked, and then stained with Alexa Fluor 546-conjugated phalloidin (Molecular Probes) after 1 h and F-actin is labeled with a fluorescence dye. Images are collected by confocal laser scanning microscopy with a 40× objective lens (Leica). (JEM (2005), 201, 647-657).

US10800844B2. Anti-Siglec-9 antibodies and methods of use thereof (2020-10-13). ALECTOR LLC [US]. Inventors: Arnon Rosenthal [US], Kate Monroe [US], Seung-Joo Lee [US].

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Visualizing TREM2-Mediated F-Actin Formation

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Example 9

It is believed that agonistic anti-TREM2, anti-DAP12, and/or TREM2/DAP12 bispecific antibodies may induce F-actin in microglial cells, macrophages, and dendritic cells.

Microglia and other cells of interest that are transduced with TREM2 or that express TREM2 are added to culture plates and then exposed to agonistic anti-TREM2, anti-DAP12, and/or TREM2/DAP12 bispecific antibodies, or a control antibody. Cells are fixed, blocked, and then stained with Alexa Fluor 546-conjugated phalloidin (Molecular Probes) after 1 h and F-actin is labeled with a fluorescence dye. Images are collected by confocal laser scanning microscopy with a 40× objective lens (Leica). (JEM (2005), 201, 647-657).

US11084875B2. Anti-TREM2 antibodies and methods of use thereof (2021-08-10). ALECTOR LLC [US]. Inventors: Kate Monroe [US], Tina Schwabe [US], Francesca Avogadri-Connors [US], Ilaria Tassi [US], Helen Lam [US], Arnon Rosenthal [US].

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Agonistic Anti-TREM1 Induces F-actin in Immune Cells

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Example 33

It is believed that agonistic anti-TREM1, or TREM1 bispecific antibodies may induce F-actin in microglial cells, macrophages, and dendritic cells. Microglia, macrophages or dendritic cells and other cells of interest that are transduced with TREM1 or that express TREM1 are added to culture plates and then exposed to agonistic anti-TREM1, and/or TREM1 bispecific antibodies, or a control antibody. Cells are fixed, blocked, and then stained with Alexa Fluor 546-conjugated phalloidin (Molecular Probes) after 1 h and F-actin is labeled with a fluorescence dye. Images are collected by confocal laser scanning microscopy with a 40× objective lens (Leica). (JEM (2005), 201, 647-657).

US11472877B2. Anti-TREM1 antibodies and methods of use thereof (2022-10-18). Alector LLC [US]. Inventors: Andrew Pincetic [US], Arnon Rosenthal [US], Helen Lam [US], Francesca Avogadri-Connors [US], Seung-Joo Lee [US].

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Visualizing Autophagy with Acridine Orange

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To further study the autophagy process, we evaluated the formation of acidic vesicular organelles, a morphological feature of autophagy [41 (link)]. An acidic cell compartment was detected by the metachromatic dye acridine orange (AO). SK-MEL-28 and CHL-1 cells (50,000 cells/well) were seeded in a 24-well plate before treatment with EcTI (50 µM and 100 µM for 24 h) or EBSS medium (for 3 h, positive control). The cells were then stained with AO (0.5 μg/mL) for 15 min. The samples were observed using a fluorescence microscope with excitation at 488 nm. Green (emission: 530–550 nm) and red (emission: 650 nm) fluorescence were examined using a 40× objective lens (Leica, Wetzlar, Germany).

Bonturi C.R., Salu B.R., Bonazza C.N., Sinigaglia R.D., Rodrigues T., Alvarez-Flores M.P., Chudzinski-Tavassi A.M, & Oliva M.L. (2022). Proliferation and Invasion of Melanoma Are Suppressed by a Plant Protease Inhibitor, Leading to Downregulation of Survival/Death-Related Proteins. Molecules, 27(9), 2956.

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Siglec-7 Antibody-Induced F-Actin in Immune Cells

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Example 16

The purpose of this Example is to test whether antagonistic anti-Siglec-7 antibodies, or Siglec-7 bispecific antibodies induce F-actin in microglial cells, macrophages, neutrophils, NK cells, and dendritic cells.

Microglia, macrophages, neutrophils, NK cells, or dendritic cells and other cells of interest that are transduced with Siglec-7 or that express Siglec-7 are added to culture plates and then exposed to antagonistic anti-Siglec-7 and/or Siglec-7 bispecific antibodies, or a control antibody. Cells are fixed, blocked, and then stained with Alexa Fluor 546-conjugated phalloidin (Molecular Probes) after 1 h and F-actin is labeled with a fluorescence dye. Images are collected by confocal laser scanning microscopy with a 40× objective lens (Leica). (JEM (2005), 201, 647-657).

US11390680B2. Anti-Siglec-7 antibodies and methods of use thereof (2022-07-19). Alector LLC [US]. Inventors: Kate Monroe [US], Helen Lam [US], Arnon Rosenthal [US], Seung-Joo Lee [US], Francesca Avogadri-Connors [US], William Monteith [US].

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Immunofluorescence Staining of Cultured Cells

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Cultured cells were fixed with 4% paraformaldehyde, washed twice with phosphate-buffered saline (PBS), and then blocked with PBS containing 10% normal goat serum. Cells were then stained with an anti-E-cadherin, anti-vimentin, or anti-FSP-1 polyclonal antibody solution for 30 min at 37 °C, washed twice with PBS, stained with a Cy3-conjugated secondary antibody for 30 min at 37 °C, and washed twice with PBS. All immunofluorescence images were captured using a DM4000 microscope (LEICA, Wetzlar, Germany) equipped with either a 20× or a 40× objective lens (LEICA) and a DFC450 C camera (LEICA). Images were processed using the LAS V4.5 controller software (LEICA).

Wu D.M., Liu T., Deng S.H., Han R., Zhang T., Li J, & Xu Y. (2020). Alpha-1 Antitrypsin Induces Epithelial-to-Mesenchymal Transition, Endothelial-to-Mesenchymal Transition, and Drug Resistance in Lung Cancer Cells. OncoTargets and therapy, 13, 3751-3763.

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Ex Situ Raman Analysis of Galvanostatic Cycling

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Ex situ measurements
were performed
at different stages of galvanostatic cycling. To avoid oxygen and
moisture contamination, an airtight Raman cell (ECC-Opto-STD, El-Cell,
GmBH) was assembled inside an argon-filled glovebox. Raman spectra
were collected with a 633 nm wavelength laser using a Raman system
(Renishaw inVia Reflex) with a microscope focused through a 50×
objective lens (Leica). The estimated power on the sample was 0.43
mW with 200 s exposure time and two accumulations. The baseline of
the spectra was corrected for clarity.

Taylor Z.N., Perez A.J., Coca-Clemente J.A., Braga F., Drewett N.E., Pitcher M.J., Thomas W.J., Dyer M.S., Collins C., Zanella M., Johnson T., Day S., Tang C., Dhanak V.R., Claridge J.B., Hardwick L.J, & Rosseinsky M.J. (2019). Stabilization of O–O Bonds by d0 Cations in Li4+xNi1–xWO6 (0 ≤ x ≤ 0.25) Rock Salt Oxides as the Origin of Large Voltage Hysteresis. Journal of the American Chemical Society, 141(18), 7333-7346.

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Raman Spectroscopy for Biological Analyte Detection

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The Renishaw inVia Raman spectrometer coupled to a research-grade Leica microscope was employed in this study, and specifically, the helium-neon laser (633 nm) was used for excitation. The suitable grating and filter combinations were installed, and calibration was conducted before each set of measurements. For the Raman measurements, the sample was inverted on the microscope stage to allow the laser to be aligned, positioned and focussed on the sample through the backside if the slide, which has been shown to increase the SERS enhancement. 55 (link) The parameters for all sets of measurements were the same, including a 10 s or 30 s integration time, 25 mW or 5 mW laser power on the sample and the spectral range was from 500 to 1750 cm -1 . The Raman spectra were collected by a 50× objective lens from Leica. Fluorescence in the collected spectra was removed by a background subtraction algorithm by Wire 5.4, followed by a process using MATLAB R2018b software package. 56 (link) To assess the fouling effect of the unprocessed whole blood on the Raman spectra of R6G, and the undiluted milk on the Raman spectra of cysteine, 20 µl of each of R6G spiked in 150 mM NaCl or blood, and cysteine spiked in 150 mM NaCl or undiluted milk was deposited on the bare SERS substrate,

Han M., Silva S.M., Russo M.J., Desroches P.E., Lei W., Quigley A.F., Kapsa R.M.I., Moulton S.E., Stoddart P.R, & Greene G.W. (2023). Lubricin (PRG-4) anti-fouling coating for surface-enhanced Raman spectroscopy biosensing: towards a hierarchical separation system for analysis of biofluids. The Analyst, 149(1).

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