Abundant plasma protein removal

Example 14

Two plasma samples from a single preterm infant, one from the umbilical cord blood and one from a peripheral blood sample taken on ex utero day 28, were subjected to abundant plasma protein removal (NORGEN™) prior to 2D-electrophoresis, using separation first by isoelectric focusing (pH range 3-8) and then by size (TGX™ precast gel, BIO-RAD™). The resulting gels were compared for differential protein content. Six differentially expressed protein clusters (‘spots’) were sent to the University of Utah Proteomics Core for analysis. Following trypsin digestion and tandem mass spectroscopy using an LTQ-FT ion-trap/FTMS hybrid mass spectrometer (THERMO ELECTRON™), candidate proteins/peptides were identified as potential NET-Inhibitory Factors.

Example 14

Two plasma samples from a single preterm infant, one from the umbilical cord blood and one from a peripheral blood sample taken on ex utero day 28, were subjected to abundant plasma protein removal (NORGEN™) prior to 2D-electrophoresis, using separation first by isoelectric focusing (pH range 3-8) and then by size (TGX™ precast gel, BIO-RAD™). The resulting gels were compared for differential protein content. Six differentially expressed protein clusters (“spots”) were sent to the University of Utah Proteomics Core for analysis. Following trypsin digestion and tandem mass spectroscopy using an LTQ-FT ion-trap/FTMS hybrid mass spectrometer (THERMO ELECTRON™), candidate proteins/peptides were identified as potential NET-Inhibitory Factors.