Mir 221 mimic

Jurkat cells were seeded into 6-well plates (1x10⁶ cells/well) and cultured at 37˚C for 24 h. Then, cells were transfected with 100 nM inhibitor control (the negative control of miR-221 inhibitor; 5'-CAGUACUUUUGUGUAGUACAA-3'; Guangzhou Ribobio Co., Ltd.), 100 nM miR-221 inhibitor (miR-221 antagonist' 5'-GAAACCCAGCAGACAAUGUAGCU-3'; Guangzhou Ribobio Co., Ltd.), 50 nM mimic control (the negative control of miR-221 mimic; 5'-CGGUACGAUCGCGGCGGGAUAUC-3'; Guangzhou Ribobio Co., Ltd.), 50 nM miR-221 mimic (miR-221 agonist; 5'-AGCUACAUUGUCUGCUGGGUUUC-3'; Guangzhou Ribobio Co., Ltd.), 100 nM miR-221 inhibitor + 0.2 µM control-small interfering (si)RNA (cat. no. sc-36869; Santa Cruz Biotechnology, Inc.) or 100 nM miR-221 inhibitor + 0.2 µM PTEN-siRNA (cat. no. sc-29459; Santa Cruz Biotechnology, Inc.) using Lipofectamine 3000 reagent, according to the manufacturer's instructions. The transfection efficiency was detected 48 h later using RT-qPCR.