Phospho egfr tyr1068 antibody

Manufactured by Cell Signaling Technology

The Phospho-EGFR (Tyr1068) antibody is a tool designed to detect the phosphorylation of the epidermal growth factor receptor (EGFR) at tyrosine 1068. This antibody can be used in various applications to study the activation and signaling of EGFR.

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Lab products found in correlation

Anti egfr antibody, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Anti-phospho-EGFR Tyr1068 antibody (3 citations) Phospho-EGFR (Tyr1068) (24 citations) Phospho-EGFR (85 citations) EGFR antibody (12 citations)

3 protocols using phospho egfr tyr1068 antibody

EGFR Signaling Modulation Kinetics

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H1975, PC9 and H3255 cells were treated with CHMFL-EGFR-26 (0.01μM, 0.03μM, 0.1μM) for 4 hours before they were washed out by PBS for three times. Then cells were incubated in 10% FBS-containing RPMI for indicated time periods before they were collected and lysed. EGFR, Phospho-EGFR (Tyr1068) antibody (Cell signaling Technology) were used for immunoblotting.

Hu C., Wang A., Wu H., Qi Z., Li X., Yan X.E., Chen C., Yu K., Zou F., Wang W., Wang W., Wu J., Liu J., Wang B., Wang L., Ren T., Zhang S., Yun C.H., Liu J, & Liu Q. (2017). Discovery and characterization of a novel irreversible EGFR mutants selective and potent kinase inhibitor CHMFL-EGFR-26 with a distinct binding mode. Oncotarget, 8(11), 18359-18372.

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EGFR Phosphorylation Assay in A549 and SNU-5 Cells

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Approximately 10⁶ A549 or SNU-5 cells/well were grown overnight in six-well plates and incubated for 15 min with 30 μg/ml of antibody in the absence or presence of 40 ng/ml EGF. After cell lysis, Western blots determined EGFR phosphorylation status with phospho-EGFR (Tyr1068) antibody (Cell Signaling 2234) and total EGFR protein using an anti-EGFR antibody (Cell Signaling 2232).

Neijssen J., Cardoso R.M., Chevalier K.M., Wiegman L., Valerius T., Anderson G.M., Moores S.L., Schuurman J., Parren P.W., Strohl W.R, & Chiu M.L. (2021). Discovery of amivantamab (JNJ-61186372), a bispecific antibody targeting EGFR and MET. The Journal of Biological Chemistry, 296, 100641.

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EGFR Phosphorylation Inhibition Kinetics

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H1975 cells were treated with ibrutinib (0.3 μM, 1 μM, 3 μM), WZ4002 (0.3 μM, 1 μM) for 4 hour before they were thoroughly washed out by PBS for three times. Then cells were incubated in 10% FBS-containing RPMI1640 for indicated time before they are collected and lysed. EGFR, Phospho-EGFR (Tyr1068) antibody (Cell Signaling Technology) were used for immunoblotting.

Wang A., Yan X.E., Wu H., Wang W., Hu C., Chen C., Zhao Z., Zhao P., Li X., Wang L., Wang B., Ye Z., Wang J., Wang C., Zhang W., Gray N.S., Weisberg E.L., Chen L., Liu J., Yun C.H, & Liu Q. (2016). Ibrutinib targets mutant-EGFR kinase with a distinct binding conformation. Oncotarget, 7(43), 69760-69769.

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