The H. beimenensis NTU-111 strain used in this study was originally isolated from brine samples from the Beimen saltern in southern Taiwan22 (link). H. beimenensis was grown in basal medium containing 5 g/L yeast extract (Bacto, BD), 5 g/L Casamino acids (Bacto, BD) and 5 g/L MgSO4·7H2O (Wako), pH 7.5. To determine the optimal growth conditions for H. beimenensis, the bacteria were grown in basal medium with various concentrations of NaCl, including 0%, 5%, 10%, 15%, 20%, and 25% NaCl (w/v), and were incubated at 37 °C with shaking at 220 rpm. The concentration of H. beimenensis (OD600) was monitored by a spectrophotometer (Libra S4, Biochrom) every 6 h until 72 h with three replicates.
Libra s4
Manufactured by Harvard Bioscience
Sourced in United Kingdom
The Libra S4 is a high-performance spectrophotometer designed for accurate and precise UV-Vis absorbance measurements. It features a compact design, dual-beam optical system, and a wavelength range of 190 to 1100 nm.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using libra s4
1
Optimizing Growth Conditions for Haloarchaea
2
Halotolerance of Vibrio chiguensis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
V. chiguensis was incubated in growth medium [5 g/L yeast extract (Bacto, BD), 5 g/L casamino acids (Bacto, BD) and 5 g/L MgSO4·7H2O (Wako), pH 7.5] at 37°C with shaking at 220 rpm. For the halotolerant evaluation, the bacteria were incubated with various NaCl concentrations (0%, 5%, 10%, 15%, 20%, 25%) in the growth medium. To monitor the V. chiguensis concentration, a spectrophotometer (Libra S4, Biochrom) was used to measure three replicates per condition at OD600 every 6 h until 48 h.
3
Biomass Concentration Determination
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Biomass concentration was determined by optical density (OD600 nm) measurement at 600 nm (spectrophotometer Libra S4, Biochrom, Cambridge, UK) with a 2 mm absorption cell (Hellma, Müllheim, Germany) and cell dry weight measurements. Cell dry weight was estimated by filtration on polyamide membrane (Sartolon 0.2 µm-Sartorius®, Sartorius, Göttingen, Germany) and drying to a constant weight for 48 h, at 60 °C under 200 mmHg in a vacuum oven (Heraeus, Hanau, Germany).
4
RNA Extraction and Microarray Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For RNA extraction and microarray analysis, cells were grown in continuous culture at 37 °C in M9 minimal medium supplemented with glucose as described3 (link). The MG1655, MG1655(csrA51) and MG1655(ΔcsrD) strains were cultured at the same growth rate, μ = 0.10 h−1, which corresponds to doubling time of 6.9 h. Each culture was repeated three times to provide independent biological replicates. Biomass was estimated from absorbance at 600 nm (Libra S4, Biochrom): 1 unit of absorbance corresponding to 0.42 g of dry cell weight l−1 for the MG1655 strain, 0.27 g of dry cell weight l−1 for the MG1655(csrA51) strain and 0.44 g of dry cell weight l−1 for the MG1655(ΔcsrD) strain. To determine the maximal growth rate of each strain, the strains were grown in batch culture (identical medium, oxygen concentration, pH and temperature) and the rates were determined in exponential growth phase.
5
Shake-Flask Fermentation of Yeast
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Shake-flask fermentations were carried out at 30 °C, 200 rpm with aluminium foil used to cover flask tops and medium making up 10% of the baffled flask volume. Single colonies from solid CBS agar plates that had been streaked with glycerol-stocked strains were used to inoculate 10 mL of liquid CBS medium. After 24 h growth, cells were passaged into a second pre-culture (25 mL) and grown to mid to late log phase (OD660 nm of 1–5) prior to inoculation of the experimental culture (50 mL) at an OD660 nm of 0.4. Synthetic alpha-pheromone (Genscript, Piscataway, NJ, USA) was added to flasks at a final concentration of 1 µM at indicated time points. Samples for analysis of extracellular metabolites were obtained by centrifugation of 1 mL of culture at 13,000×g for 7 min at 4 °C and storing the supernatant at −20 °C until analysis. Population density was measured using absorbance at 660 nm (OD660nm) on a spectrophotometer (LibraS4, Biochrom UK). OD660 nm values were converted to biomass using a conversion factor of 0.243 g dry cell weight per 1 OD unit (determined using exponentially growing CEN.PK113-5D populations).
6
Antibacterial Effects of ZnO NPs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Turbidity test was conducted to investigate the antibacterial effect of ZnO NPs on S. marcescens and E. faecalis by determining the optical density of bacterial suspensions treated with 5, 10, 20, 40, 80, and 160 μg/ml of ZnO NPs by spectrophotometer at 600 nm (Libra S4, Biochrom, UK) along with negative and positive controls. LB broth was used as the blank. The absorbance from the respective concentration of ZnO NPs was subtracted from the test readings to avoid the interference by the NPs. Then, the absorbance values were compared to calculate the percentage of bacterial growth inhibition using equation 1 (Eq. 1).
Percentage of growth inhibition = (OD600 negative control–OD600 test)/OD600 negative control × 100 (Eq.1).
Percentage of growth inhibition = (OD600 negative control–OD600 test)/OD600 negative control × 100 (Eq.1).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!