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Tsc sp5 2 confocal microscope

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The Leica TSC SP5 II is a high-performance confocal microscope designed for advanced imaging applications. It features a modular design, allowing for customization to meet specific research needs. The system provides high-resolution, multi-channel imaging capabilities, enabling detailed analysis of samples.

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Lab products found in correlation

Eclipse ti e inverted microscope, by Leica (2 mentions) Fluoromount, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

SP5 confocal microscope (1772 citations) SP5 microscope (176 citations) SP5 II confocal microscope (128 citations) SP5 II (82 citations) TSC SP5 (38 citations) TSC SP5 microscope (13 citations) SP5 II microscope (13 citations) TSC SP5 II (7 citations) Confocal SP5-II (7 citations) Confocal SP5 (6 citations)

5 protocols using tsc sp5 2 confocal microscope

1

Confocal Microscopy and Image Processing

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Sections were examined with a Leica TSC SP5 II confocal microscope (cLSM). Z-series were processed with NIH ImageJ, v. 1.8 (Rasband WS, ImageJ, U.S. National Institutes of Health, Bethesda, MD, http://rsb.info.nih.gov/ij/), producing maximum projections. Images were processed in Adobe Photoshop 6.0 (San Jose, CA) using global contrast and brightness adjustment features as well as black and white inversion.
Maurer M., Hladik J., Iliffe T.M, & Stemme T. (2019). Histaminergic interneurons in the ventral nerve cord: assessment of their value for Euarthropod phylogeny. Zoological Letters, 5, 36.
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2

Immunofluorescence Staining Protocol

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Cells were washed in PBS, followed by fixation in 4% PFA for 10 minutes, and washed again in PBS prior to antibody labeling. Cells were first permeabilized with 0.25% Triton X-100 for 5 minutes and then blocked with 10% serum for up to 2 hours. Primary antibody labeling was performed overnight at 4°C in 3% serum. Primary antibody was washed and replaced with secondary antibody in 3% serum at room temperature for 60 minutes. The cells were then washed and counterstained with DAPI before mounting on slides. Images were acquired on a Nikon Eclipse Ti-E inverted microscope or on a Leica TSC SP5 II confocal microscope.
Nickolls A.R., Lee M.M., Espinoza D.F., Szczot M., Lam R.M., Wang Q., Beers J., Zou J., Nguyen M.Q., Solinski H.J., AlJanahi A.A., Johnson K.R., Ward M.E., Chesler A.T, & Bönnemann C.G. (2020). Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells. Cell reports, 30(3), 932-946.e7.
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3

Confocal Imaging and Image Processing

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Sections were examined with a Leica TSC SP5 II confocal microscope (cLSM). Z-series were processed with NIH ImageJ, v. 1.8 (Rasband WS, ImageJ, U.S. National Institutes of Health, Bethesda, MD, USA, http://rsb.info.nih.gov/ij/ (accessed on 20 November 2021)), producing maximum projections. Image processing and panel preparation were conducted with Adobe Photoshop 6.0 (San Jose, CA, USA), including global contrast and brightness adjustment.
Stemme T, & Pfeffer S.E. (2021). Anatomy of the Nervous System in Chelifer cancroides (Arachnida: Pseudoscorpiones) with a Distinct Sensory Pathway Associated with the Pedipalps. Insects, 13(1), 25.
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4

Immunofluorescence Staining Protocol

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Cells were washed in PBS, followed by fixation in 4% PFA for 10 minutes, and washed again in PBS prior to antibody labeling. Cells were first permeabilized with 0.25% Triton X-100 for 5 minutes and then blocked with 10% serum for up to 2 hours. Primary antibody labeling was performed overnight at 4°C in 3% serum. Primary antibody was washed and replaced with secondary antibody in 3% serum at room temperature for 60 minutes. The cells were then washed and counterstained with DAPI before mounting on slides. Images were acquired on a Nikon Eclipse Ti-E inverted microscope or on a Leica TSC SP5 II confocal microscope.
Nickolls A.R., Lee M.M., Espinoza D.F., Szczot M., Lam R.M., Wang Q., Beers J., Zou J., Nguyen M.Q., Solinski H.J., AlJanahi A.A., Johnson K.R., Ward M.E., Chesler A.T, & Bönnemann C.G. (2020). Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells. Cell reports, 30(3), 932-946.e7.
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5

Immunofluorescence Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed in Dulbecco’s PBS (DPBS) then incubated in 4% paraformaldehyde for 10 min at room temperature. Fixation was quenched using 0.1 M glycine solution for 5 min, followed by two washes with DPBS. Cells were permeabilized in 0.25% Triton X-100 for 5 min and blocked in 10% goat serum for 1 h at room temperature. Primary antibodies in 3% goat serum were then applied overnight at 4 °C and washed three times before incubation with secondary antibody in 3% goat serum for 1 h at room temperature. Cells were washed, stained with DAPI and mounted with Fluoromount (Thermo Fisher). Images were taken using a Leica TSC SP5 II confocal microscope.
Mohassel P., Donkervoort S., Lone M.A., Nalls M., Gable K., Gupta S.D., Foley A.R., Hu Y., Saute J.A., Moreira A.L., Kok F., Introna A., Logroscino G., Grunseich C., Nickolls A.R., Pourshafie N., Neuhaus S.B., Saade D., Gangfuß A., Kölbel H., Piccus Z., Le Pichon C.E., Fiorillo C., Ly C.V., Töpf A., Brady L., Specht S., Zidell A., Pedro H., Mittelmann E., Thomas F.P., Chao K.R., Konersman C.G., Cho M.T., Brandt T., Straub V., Connolly A.M., Schara U., Roos A., Tarnopolsky M., Höke A., Brown R.H., Lee C.H., Hornemann T., Dunn T.M, & Bönnemann C.G. (2021). Childhood amyotrophic lateral sclerosis caused by excess sphingolipid synthesis. Nature medicine, 27(7), 1197-1204.
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