Cd36 pe

Manufactured by BioLegend

Sourced in United Kingdom, Germany, United States

CD36-PE is a fluorescently-labeled antibody that binds to the CD36 surface antigen. CD36 is a transmembrane glycoprotein that functions as a receptor for various ligands, including thrombospondin, long-chain fatty acids, and oxidized low-density lipoproteins. The PE fluorescent label allows for the detection and quantification of CD36-expressing cells using flow cytometry or other fluorescence-based techniques.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

PE anti-human CD36 (2 citations) Anti-CD36–PE (2 citations)

7 protocols using cd36 pe

Identifying Immune Cell Subsets by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were washed to remove DTT and stained with the indicated antibodies: HLA-DR-APC (BD Bioscience, Oxford, UK), CD16-PE, CD36-PE, CD15-APC (Biolegend, San Diego, CA) and CD14-biotin (Southern Biotech, Birmingham, AL). Staining combinations were as follows: CD15, CD14/CD16, CD14/HLA-DR/CD36. Streptavidin-PE-Cy5.5 tandem conjugate (eBioscience) was used as a secondary reagent to detect biotinylated primary antibodies. Following staining, samples were immediately analysed by flow cytometry (FACScalibur, Becton Dickinson, Oxford, UK).
Neutrophils were identified as CD15⁺ events. Cells of the monocyte/macrophage lineage were identified as CD14⁺. CD15⁺ neutrophils were CD14 negative, allowing distinction of these two cell types [10] (link). CD14⁺/16⁺⁺ non-classical monocytes were identified as highly CD16 positive CD14⁺ cells. Forward scatter (FSC) and side scatter (SSC) profiles were also examined. Flow cytometry data were analysed using FlowJo (Tree Star Inc, Ashland, OR).

Prince L.R., Maxwell N.C., Gill S.K., Dockrell D.H., Sabroe I., McGreal E.P., Kotecha S, & Whyte M.K. (2014). Macrophage Phenotype Is Associated with Disease Severity in Preterm Infants with Chronic Lung Disease. PLoS ONE, 9(8), e103059.

+ Open protocol

+ Expand

Flow Cytometric Characterization of Cell Surface Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the detection of cell surface molecules, cells were incubated with the following antibodies: CD14 APC (eBioscience, Frankfurt, Germany), CD36 PE, CD38 PE, CD86 FITC, F4/80 APC, Ly6C FITC (all from BioLegend, Fell, Germany), or the appropriate isotype controls. Flow cytometric analysis was performed using FACSCalibur and analyzed using FlowJo software (Tree Star, Ashland, OR, USA).

Heming M., Gran S., Jauch S.L., Fischer-Riepe L., Russo A., Klotz L., Hermann S., Schäfers M., Roth J, & Barczyk-Kahlert K. (2018). Peroxisome Proliferator-Activated Receptor-γ Modulates the Response of Macrophages to Lipopolysaccharide and Glucocorticoids. Frontiers in Immunology, 9, 893.

+ Open protocol

+ Expand

Flow Cytometry for Murine CD4 T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used for flow cytometry in this study: CD45-PE-Cy7 (clone 30-F11; BioLegend); CD4-eFluor 450, -PerCP-Cy5.5, -APC (clone RM4–5; eBioscience); Thy1.1-PerCP (clone OX-7; BD Pharmigen); Thy1.1-PerCP-Cy5.5 (clone HIS51; Invitrogen); Thy1.2-APC, -APC-eFluor780 (clone 53–2.1; eBioscience); MHCII (IA/IE), -APC (clone M5/114.15.2; eBioscience); CD36-PE (clone HM36; BioLegend); CD27-BV510 (clone LG.3A10; BioLegend); CD127-PE (clone SB/199; eBioscience); CD25-PE-Cy7 (clone PC61.5; eBioscience); CD3-BV510 (clone 17A2, Biolegend); and IFNγ-PerCP-Cy5.5 (clone XMG1.2; BioLegend). CellTrace Violet (Invitrogen) was used to stain cells in vivo competition assays. Data were acquired using a FACSVerse (BD Biosciences) flow cytometer and analyzed using FlowJo software (Tree Star). Cells were sorted using a FACSAria Fusion or FACSAria II (BD Biosciences) flow cytometer. Prior to FACS sorting, CD4 T cells were pre-enriched from spleens using a magnet-based CD4 T cell Isolation Kit (Miltenyi) and autoMACS cell separator (Miltenyi) per manufacturer instructions to reduce FACS time.

Sariol A., Zhao J., Abrahante J.E, & Perlman S. (2022). Virus-specific regulatory T cells persist as memory in a neurotropic coronavirus infection. Journal of immunology (Baltimore, Md. : 1950), 208(8), 1989-1997.

+ Open protocol

+ Expand

Flow Cytometry Analysis of Immune Cell Subsets

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometry was performed as described (6 (link)). Cells were incubated with anti-mouse CD16/32 (Fc Block; BD Biosciences) before staining with primary antibody or isotype controls. Ten-thousand to 50,000 events per sample were acquired using an LSRII flow cytometer (BD-Biosciences) and analyzed with Flowjo flow cytometry software (Tree Star Inc.). The following antibodies were used: CD11b-Brilliant Violet 421, Tim4-PE, CD138-APC, CD138-PE F4/80- Pacific Blue, Ly6G-APC-Cy7, Ly6C-APC-Cy7, CD80-PerCp-Cy5, CD40-PerCP-Cy5, CD206- PerCP-Cy5, CCR2-FITC, CX3CR1-FITC, CD169-APC, CCR5-APC, CD36-PE, CD36-AlexaFluor 488, and IL-10R-PE (Biolegend); Marco-FITC (Biorad); Ly6C-FITC, CD86-FITC, CD11c-FITC, I-A/I-E-PE, CD93-PE (BD Bioscience); CREB-PE and Phospho-CREB-FITC (Cell Signaling); CD11b-Pacific Blue, CD45-FITC, TLR4-PE, CD45-FITC (eBioscience). Buffers used in intracellular staining were from eBioscience. For cell-sorting, PEC from untreated B6 mice were stained with anti-CD11b, Tim4, and CD138 antibodies. CD11b⁺Tim4⁺ and CD11b⁺CD138⁺ cells were gated and sorted (FACSaria cell sorter). Forty-thousand cells/subset were collected and lysed immediately for RNA extraction.

Han S., Zhuang H., Shumyak S., Wu J., Li H., Yang L.J, & Reeves W.H. (2017). A novel subset of anti-inflammatory CD138+ macrophages is deficient in mice with experimental lupus*. Journal of immunology (Baltimore, Md. : 1950), 199(4), 1261-1274.

+ Open protocol

+ Expand

Phenotyping Murine Bone Marrow-Derived Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols

BMDMs were obtained from control and ketamine-treated mice as previously described [35] . Briefly, cells were cultured in RPMI 1640 supplemented with 13% supernatant of the mouse L929 cell line (conditioned medium) for 7 days. Then, cells were stimulated with LPS (100 ng/mL) or maintained in media for 24 h. Cells were harvested and stained with CD86-APC/Cy7 (BioLegend Cat # 105,029, RRID: AB_2074993), CD206-PE/Cy7 (BioLegend Cat # 141719, RRID: AB_2562247), and CD36-PE (Biolegend Cat # 102605, RRID: AB_389348) antibodies before analysis by flow cytometry with the FACS Canto II cytometre (Becton Dickinson).

Nowak W., Grendas L.N., Sanmarco L.M., Estecho I.G., Arena Á.R., Eberhardt N., Rodante D.E., Aoki M.P., Daray F.M., Carrera Silva E.A, & Errasti A.E. (2019). Pro-inflammatory monocyte profile in patients with major depressive disorder and suicide behaviour and how ketamine induces anti-inflammatory M2 macrophages by NMDAR and mTOR. EBioMedicine, 50, 290-305.

+ Open protocol

+ Expand

Microglial Immunophenotyping from Brain

Check if the same lab product or an alternative is used in the 5 most similar protocols

Meninges discarded tissue samples were transported immediately to the laboratory for processing. The tissue was dissociated (Brain Dissociation kit, Miltenyi). The dissociated cells underwent myelin removal (Myelin removal kit, Miltenyi) and CD11b+ cells were further purified (CD11b microglia Microbeads, Miltenyi). All kits were used according to the manufacturers instructions. The cells were then surface stained with fluorescently conjugated antibodies for microglial markers: CD11b-APC, CD45-Bv605 and P2RY12-Bv421, CD26-FITC, and CD36-PE (Biolegend). The cells were incubated with antibodies for 30 min on ice, washed, resuspended in 500 μL staining buffer, and acquired on a FACS Fortessa (BD Biosciences). Results were analyzed using FlowJo software.

Lustig G., Cele S., Karim F., Derache A., Ngoepe A., Khan K., Gosnell B.I., Moosa M.Y., Ntshuba N., Marais S., Jeena P.M., Govender K., Adamson J., Kløverpris H., Gupta R.K., Harrichandparsad R., Patel V.B, & Sigal A. (2021). T cell derived HIV-1 is present in the CSF in the face of suppressive antiretroviral therapy. PLoS Pathogens, 17(9), e1009871.

+ Open protocol

+ Expand

Flow Cytometric Immunophenotyping of HSPCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

HSPCs were incubated with fluorescence-conjugated antibodies (CD33-PE, CD34-FITC, or CD36-PE) purchased from Biolegend (San Diego, CA, USA) for 30 min on ice. The antibody-treated HSPCs were resuspended in FACS buffer (0.1% BSA and EDTA in PBS) and analyzed using a flow cytometer (BD FACS CANTO II) and the CellQuest program (BD Biosciences, San Jose, CA, USA). A dataset of 10,000 cells was acquired.

Hwang Y.J., Shin D.Y., Kim M.J., Jang H., Kim S., Yang H., Jang W.I., Park S., Shim S, & Lee S.B. (2023). StemRegenin 1 Mitigates Radiation-Mediated Hematopoietic Injury by Modulating Radioresponse of Hematopoietic Stem/Progenitor Cells. Biomedicines, 11(3), 824.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!