Il 17a pe

Manufactured by Beckman Coulter

Sourced in United States

IL-17A-PE is a fluorescent-labeled antibody used for the detection and analysis of IL-17A, a cytokine involved in inflammatory responses. It is designed for use in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Cd86 fitc, by Thermo Fisher Scientific (1 mentions) Efluor 506, by Thermo Fisher Scientific (1 mentions) Lps e coli 055 b5, by Merck Group (1 mentions) Cd80 pe, by Thermo Fisher Scientific (1 mentions) Cli 095, by InvivoGen (1 mentions) Cd40 pe, by BioLegend (1 mentions) Cd54 apc, by Thermo Fisher Scientific (1 mentions) Cd25 fitc, by Thermo Fisher Scientific (1 mentions) Cytokines, by Miltenyi Biotec (1 mentions) Cpg odn 2006, by InvivoGen (1 mentions) Hla dr apc, by BD (1 mentions) Cd4 percp, by BioLegend (1 mentions) Protein a agarose beads, by Cell Signaling Technology (1 mentions) Ifn γ fitc, by Thermo Fisher Scientific (1 mentions) Il 4 pe, by Thermo Fisher Scientific (1 mentions) Cd4 fitc, by Beckman Coulter (1 mentions) Cd45 fitc, by Beckman Coulter (1 mentions) Phytohemagglutinin (pha), by Merck Group (1 mentions) Cd29 pe, by Beckman Coulter (1 mentions) Cd25 pc5, by Beckman Coulter (1 mentions)

2 protocols using il 17a pe

Flow Cytometry Immunophenotyping Protocol

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For flow cytometry, the following fluorochrome-conjugated monoclonal antibodies were used. BD Biosciences: HLA-DR-APC (Clone: G46-6), CD86-FITC (Clone: FUN-1), CD80-PE (Clone: L307.4), CD54-APC (Clone: HA58), CD25-FITC (Clone: M-A251), CD127-BV421 (clone HIL-7R-M21), IFN-γ-FITC (Clone: 4S.B3), IL-4-PE (Clone: MP4-25D2); eBioscience: FoxP3-APC (Clone: 236A/E7), IL-17A-PE (Clone: Ebio64cap17); Beckman Coulter: CD40-PE (Clone: MAB89); Biolegend: CD4-PerCP (Clone: SK3). Cell viability was detected using the fixable viability dye eFluor 506 (eBioscience).
Antigen affinity-purified polyclonal anti-human TLR4 goat IgG was purchased from R&D systems. Cytokines (recombinant human granulocyte-macrophage colony-stimulating factor and IL-4), MicroBeads (CD14 and CD4) and cell purification units were obtained from Miltenyi Biotec. Protein-A agarose beads were from Cell Signalling Technology, and E. coli 055:B5 LPS and Polymyxin B-conjugated agarose beads were from Sigma-Aldrich. TLR4 signaling inhibitor CLI-095 and CpG ODN 2006 were procured from InvivoGen.

Mukherjee S., Karnam A., Das M., Babu S.P, & Bayry J. (2019). Wuchereria bancrofti filaria activates human dendritic cells and polarizes T helper 1 and regulatory T cells via toll-like receptor 4. Communications Biology, 2, 169.

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Isolation and Culture of Mesenchymal Stem Cells

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Mesenchymal stem cell culture medium (MSCM) was purchased from Sciencell, USA. Lymphocyte separation medium (Ficoll, 1.077 g/mL) was purchased from GE Healthcare Life Sciences, USA. Phytohemagglutinin (PHA) was purchased from Sigma, USA. Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) fluorescent dye was purchased from the VICMED Company, Xuzhou, Jiangsu, China. Mouse anti-human CD34-PE, CD29-PE, CD44-FITC, CD45-FITC, CD127-PE, CD4-FITC, CD25-PC5, CD3-FITC, CD8-PC5, and IL-17A-PE monoclonal antibodies, and the flow cytometer were acquired from Beckman-Coulter, USA.

Guo C.H., Han L.X., Wan M.R., Deng G.J, & Gan J.H. (2015). Immunomodulatory effect of bone marrow mesenchymal stem cells on T lymphocytes in patients with decompensated liver cirrhosis. Genetics and molecular research : GMR, 14(2).

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