The obtained protein fractions were analyzed using standard SDS-polyacrylamide gel electrophoresis (PAGE), followed by Coomassie Brilliant Blue R-250 (Sigma-Aldrich) staining or Western blot. We used All Blue (Bio-Rad Laboratories) and Dual Xtra (Bio-Rad Laboratories) as molecular weight markers. Separated proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Immobilon-P, Millipore), which was probed with polyclonal anti-human C-terminal AMCase27 (link), anti-mouse N-terminal AMCase, anti- mouse C-terminal AMCase27 (link), polyclonal goat anti-pepsin C (I-19) antibody (Santa Cruz) or monoclonal anti-β-actin (clone AC-15) (Sigma-Aldrich), followed by peroxidase-conjugated AffiniPure F (ab’)2 Fragment Donkey Anti-Rabbit IgG (H+L) (Jackson ImmunoResearch Laboratories), AffiniPure Donkey Anti-Goat IgG-HRP (Jackson ImmunoResearch laboratories) or AffiniPure Donkey Anti-Mouse IgG (H+L) (Jackson ImmunoResearch laboratories). Bound antibodies were detected using Immobilon Western Chemiluminescent HRP Substrate (Millipore). The immunoblots were analyzed and quantified using the Luminescent Image Analyzer (ImageQuant LAS 4000, GE Healthcare) according to the manufacturer’s instructions.
Affinipure donkey anti goat igg hrp
Manufactured by Jackson ImmunoResearch
Sourced in United States
AffiniPure Donkey Anti-Goat IgG-HRP is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed to detect and visualize goat immunoglobulin G (IgG) in various immunoassays and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Affinipure f ab 2 fragment donkey anti rabbit igg h l, by Jackson ImmunoResearch (4 mentions)
Imagequant las 4000 luminescent image analyzer, by GE Healthcare (4 mentions)
Las 4000, by Cytiva (4 mentions)
Immobilon p, by Merck Group (2 mentions)
Coomassie brilliant blue r 250, by Merck Group (2 mentions)
Coomassie brilliant blue r 250 cbb, by Merck Group (2 mentions)
Monoclonal anti β actin clone ac 15, by Merck Group (1 mentions)
Affinipure donkey anti mouse igg h l, by Jackson ImmunoResearch (1 mentions)
Immobilon western chemiluminescent hrp substrate, by Merck Group (1 mentions)
4 protocols using affinipure donkey anti goat igg hrp
1
Protein Fractionation and Immunoblotting Analysis
2
Analyzing Porcine Chia and Pepsin Proteins
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The obtained protein fractions were analyzed using standard SDS-PAGE, followed by Coomassie Brilliant Blue R-250 (CBB, Sigma-Aldrich) or WB using anti-porcine N-terminal Chia (rabbit)12 (link) anti-mouse C-terminal Chia (rabbit)44 (link) or anti-porcine pepsin antibody (donkey) (GeneTex, Irvine, CA, USA), followed by peroxidase-conjugated AffiniPure F (ab’)2 Fragment Donkey Anti-Rabbit IgG (H + L) (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) or AffiniPure Donkey Anti-Goat IgG-HRP (Jackson ImmunoResearch laboratories). The immunoblots were analyzed by Luminescent Image Analyzer (ImageQuant LAS 4000, GE Healthcare, Piscataway, NJ, USA) according to the manufacturer’s instructions.
3
Protein Fractionation and Immunoblotting Analysis
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The obtained protein fractions were analyzed using standard SDS-PAGE, followed by Coomassie Brilliant Blue R-250 (CBB, Sigma-Aldrich, St. Louis, MO, USA) or Western blot using polyclonal anti-mouse C-terminal AMCase33 (link) or polyclonal pig anti-pepsin antibody (GeneTex, Irvine, CA, USA), followed by peroxidase-conjugated AffiniPure F (ab’)2 Fragment Donkey Anti-Rabbit IgG (H + L) (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) or AffiniPure Donkey Anti-Goat IgG-HRP (Jackson ImmunoResearch laboratories). The immunoblots were analyzed and quantified by Luminescent Image Analyzer (ImageQuant LAS 4000, GE Healthcare, Piscataway, NJ, USA) according to the manufacturer’s instructions.
4
Protein Analysis via SDS-PAGE and Western Blot
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We analyzed the protein fractions using standard SDS-PAGE, followed by Coomassie Brilliant Blue R-250 (Sigma-Aldrich) or Western blot. Separated proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Immobilon-P, Merck Millipore, Tokyo, Japan), which was probed using polyclonal anti-pig pepsin antibody (GeneTex, Irvine, CA, USA) or polyclonal anti-pig Chia, followed by incubation with horseradish peroxidase conjugated secondary antibodies of AffiniPure Donkey Anti-Goat IgG-HRP (Jackson ImmunoResearch laboratories) or AffiniPure F (ab’)2 Fragment Donkey Anti-Rabbit IgG (H + L) (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA). We analyzed and quantified the immunoblots using Luminescent Image Analyzer (ImageQuant LAS 4000, GE Healthcare, Piscataway, NJ, USA).
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